Molecular characterization of the Newcastle disease virus that caused an outbreak in backyard birds in Costa Rica in 2015
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| Formato: | Online |
| Idioma: | eng spa |
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Universidad Nacional, Costa Rica
2023
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| Acceso en línea: | https://www.revistas.una.ac.cr/index.php/veterinaria/article/view/17866 |
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VETERINARIA17866 |
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ojs |
| institution |
Universidad Nacional de Costa Rica |
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Ciencias Veterinarias |
| language |
eng spa |
| format |
Online |
| author |
León, Bernal Cordero-Solórzano, Juan M. Chacón, Idania Aguilar, Olga Chaves, Guisella Guzmán, Mónica Carvajal, Fabián Chaves, Ronaldo |
| spellingShingle |
León, Bernal Cordero-Solórzano, Juan M. Chacón, Idania Aguilar, Olga Chaves, Guisella Guzmán, Mónica Carvajal, Fabián Chaves, Ronaldo Molecular characterization of the Newcastle disease virus that caused an outbreak in backyard birds in Costa Rica in 2015 |
| author_facet |
León, Bernal Cordero-Solórzano, Juan M. Chacón, Idania Aguilar, Olga Chaves, Guisella Guzmán, Mónica Carvajal, Fabián Chaves, Ronaldo |
| author_sort |
León, Bernal |
| title |
Molecular characterization of the Newcastle disease virus that caused an outbreak in backyard birds in Costa Rica in 2015 |
| title_short |
Molecular characterization of the Newcastle disease virus that caused an outbreak in backyard birds in Costa Rica in 2015 |
| title_full |
Molecular characterization of the Newcastle disease virus that caused an outbreak in backyard birds in Costa Rica in 2015 |
| title_fullStr |
Molecular characterization of the Newcastle disease virus that caused an outbreak in backyard birds in Costa Rica in 2015 |
| title_full_unstemmed |
Molecular characterization of the Newcastle disease virus that caused an outbreak in backyard birds in Costa Rica in 2015 |
| title_sort |
molecular characterization of the newcastle disease virus that caused an outbreak in backyard birds in costa rica in 2015 |
| title_alt |
Caracterización molecular del virus de la enfermedad de Newcastle que ocasionó un brote en aves de traspatio en Costa Rica en 2015 Caracterização molecular do vírus da doença de Newcastle que causou um surto em aves domésticas na Costa Rica em 2015 |
| publisher |
Universidad Nacional, Costa Rica |
| publishDate |
2023 |
| url |
https://www.revistas.una.ac.cr/index.php/veterinaria/article/view/17866 |
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VETERINARIA178662024-07-09T22:29:52Z Molecular characterization of the Newcastle disease virus that caused an outbreak in backyard birds in Costa Rica in 2015 Caracterización molecular del virus de la enfermedad de Newcastle que ocasionó un brote en aves de traspatio en Costa Rica en 2015 Caracterização molecular do vírus da doença de Newcastle que causou um surto em aves domésticas na Costa Rica em 2015 León, Bernal Cordero-Solórzano, Juan M. Chacón, Idania Aguilar, Olga Chaves, Guisella Guzmán, Mónica Carvajal, Fabián Chaves, Ronaldo Newcastle disease genotype V backyard poultry Costa Rica enfermedad de Newcastle genotipo V aves de traspatio Costa Rica Doença de Newcastle genótipo V aves de fundo de quintal Costa Rica Costa Rica gained its Newcastle Disease Virus NDV-free status with vaccination according to OIE proceedings in 1996, and its declaration as a country free of the velogenic, viscerotropic form of this disease (G/SPS/GEN/119) presented to the World Trade Organization (WTO) in 1999. On April 24th, 2015, SENASA (National Animal Health Service) attended a velogenic Newcastle disease outbreak that affected backyard chickens in a small town (Bellavista, Guanacaste) in the northern part of the country, near the Nicaraguan border. Sixty-five backyard birds died from a total of 84 exposed animals. Blood samples, cloacal swabs, tracheal swabs, cecal tonsils, lung and trachea tissues were collected for diagnosis at the National Veterinary Services Laboratory (LANASEVE). These samples were screened for Avian Influenza (AIV) and NDV. All samples were negative for Avian Influenza in ELISA test and RT-PCR. Serum samples were positive for NDV antibody by hemagglutination inhibition test, and tissue and swab samples were positive for NDV by conventional RT-qPCR targeting a 310 bp fragment of the virus fusion protein gene. The amino acid sequence of the protease cleavage site within the amplicon matched the sequence of a virulent strain (112RRQKRF117). The nucleotide sequence had a 98.7% identity and an e value of 4e-153 with a genotype V velogenic sequence from Belize (KF767467) and Honduras (JN872194) collected in 2008 and 2007, respectively, according to BLASTN. A total of 3604 backyard birds were euthanized in town and its surroundings (1 km), including 3495 chickens, 66 turkeys, 6 geese, and 37 ducks. The case was considered resolved, and OIE was notified in November 2015 following OIE guidelines. In April 2017, Costa Rica recovered its disease-free status through executive decree No. 40301-MAG. Costa Rica obtuvo su estatus libre del virus de la enfermedad de Newcastle (NDV) con vacunación de acuerdo con los procedimientos de la OIE en 1996 y la declaración como país libre de la forma viscerotrópica velogénica de esta enfermedad (G / SPS / GEN / 119) presentada a la Organización Mundial del Comercio (OMC) en 1999. El 24 de abril de 2015, el SENASA (Servicio Nacional de Salud Animal) atendió un brote de la enfermedad de Newcastle que afectó a gallinas de traspatio en un pequeño pueblo (Bellavista, Guanacaste), en la parte norte del país, cerca de la frontera con Nicaragua. Se reportaron sesenta y cinco aves de traspatio muertas, de un total de 84 animales expuestos. Se recolectaron muestras de sangre, hisopos cloacales y traqueales, tejidos de tonsilas cecales, pulmones y tráquea para realizar el diagnóstico en el Laboratorio Nacional de Servicios Veterinarios (LANASEVE). Estas muestras se analizaron para detectar influenza aviar (AIV) y NDV. Todas las muestras fueron negativas a influenza aviar por ELISA y RT-qPCR. Las muestras de suero fueron positivas a anticuerpos por la prueba de inhibición de la hemaglutinación y las de tejido e hisopados fueron positivas para NDV, mediante RT-PCR convencional para un fragmento de 310 pb del gen de la proteína de fusión del virus. La secuencia de aminoácidos del sitio de escisión de la proteasa dentro del amplicón coincidió con la secuencia de una cepa virulenta (112RRQKRF117). La secuencia de nucleótidos presentó un 98,7% de identidad y un valor e de 4e-153 con una secuencia velogénica de genotipo V de Belice (KF767467) y Honduras (JN872194) recolectadas en 2008 y 2007, respectivamente según BLASTN. Un total de 3604 aves de traspatio en el pueblo y sus alrededores (1 km) fueron eliminadas, 3495 pollos, 66 pavos, 6 gansos y 37 patos. El caso se consideró resuelto y se notificó a la OIE en noviembre de 2015, siguiendo las directrices de la OIE. En abril de 2017, Costa Rica recuperó su estatus libre de enfermedad mediante la promulgación del decreto ejecutivo No. 40301-MAG. A Costa Rica obteve seu status livre do vírus da doença de Newcastle (DNC) com a vacinação de acordo com os procedimentos da OIE em 1996 e a declaração como livre da forma viscerotrópica e velogênica desta doença (G/SPS/GEN/119) submetida à Organização Mundial do Comércio (OMC) em 1999. Em 24 de abril de 2015, o SENASA (Serviço Nacional de Saúde Animal) respondeu a um surto da doença de Newcastle que afetou galinhas de fundo de quintal em um pequeno povoado (Bellavista, Guanacaste), no norte do país, perto da fronteira com a Nicarágua. Sessenta e cinco aves de quintal mortas foram relatadas, de um total de 84 animais expostos. Amostras de sangue, swabs cloacais e traqueais, tecidos de amígdalas cecais, pulmões e traquéia foram coletados para o diagnóstico no Laboratório Nacional de Serviços Veterinários (LANASEVE). Essas amostras foram testadas para influenza aviária (IA) e DNC. Todas as amostras foram negativas para influenza aviária por ELISA e RT-qPCR. As amostras de soro foram positivas para anticorpos pelo teste de inibição da hemaglutinação e os tecidos e swabs foram positivos para DNC, usando RT-PCR convencional para um fragmento de 310 pb do gene da proteína de fusão do vírus. A sequência de aminoácidos do local de clivagem da protease dentro do amplicon correspondia à sequência de uma cepa virulenta (112RRQKRF117). A sequência nucleotídica apresentou 98,7% de identidade e um valor e de 4e-153 com uma sequência velogênica genótipo V de Belize (KF767467) e Honduras (JN872194) coletada em 2008 e 2007, respectivamente de acordo com o BLASTN. Um total de 3.604 aves de fundo de quintal dentro e ao redor do povoado (1 km) foram abatidas, 3.495 galinhas, 66 perus, 6 gansos e 37 patos. O caso foi considerado resolvido e notificado à OIE em novembro de 2015, seguindo as orientações da OIE. Em abril de 2017, a Costa Rica recuperou seu status de livre de doenças por meio da promulgação do decreto executivo nº 40301-MAG. Universidad Nacional, Costa Rica 2023-01-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto application/pdf text/html application/epub+zip audio/mpeg audio/mpeg https://www.revistas.una.ac.cr/index.php/veterinaria/article/view/17866 10.15359/rcv.41-1.1 Ciencias veterinarias; Vol. 41 No. 1 (2023): Ciencias Veterinarias (January-June); 1-14 Ciencias veterinarias; Vol. 41 Núm. 1 (2023): Ciencias Veterinarias (Enero-Junio); 1-14 2215-4507 0250-5649 eng spa https://www.revistas.una.ac.cr/index.php/veterinaria/article/view/17866/26867 https://www.revistas.una.ac.cr/index.php/veterinaria/article/view/17866/27056 https://www.revistas.una.ac.cr/index.php/veterinaria/article/view/17866/27057 https://www.revistas.una.ac.cr/index.php/veterinaria/article/view/17866/31146 https://www.revistas.una.ac.cr/index.php/veterinaria/article/view/17866/31147 Copyright (c) 2022 Revista Ciencias Veterinarias http://creativecommons.org/licenses/by-nc-nd/3.0/cr/ |