Noncytopathic hepatitis A virus induces surface alterations in LLC-MK2 cells revealed by thin sections, negative staining, and scanning electron microscopy

Noncytopathic hepatitis A virus induces surface alterations in LLC-MK2 cells revealed by thin sections, negative staining, and scanning electron microscopy

Los estudios previos sobre los eventos ultraestructuralesen la replicación del virus de la hepatitis Aen cultivos decélulas infectadas experimentalmente, se han realizado exclusivamentemediante cortes ultrafinos. No obstante, no sehan observado diferencias importantes entre células infectadasy no in...

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Main Authors: Hernández-Chavarría, Francisco, Alzamora-González, Libertad, Herrero-Uribe, Libia
Format: Online
Language:eng
Published: Universidad de Costa Rica 2002
Online Access:https://revistas.ucr.ac.cr/index.php/rbt/article/view/16437
id RBT16437
record_format ojs
spelling RBT164372022-05-25T21:47:13Z Noncytopathic hepatitis A virus induces surface alterations in LLC-MK2 cells revealed by thin sections, negative staining, and scanning electron microscopy Noncytopathic hepatitis A virus induces surface alterations in LLC-MK2 cells revealed by thin sections, negative staining, and scanning electron microscopy Hernández-Chavarría, Francisco Alzamora-González, Libertad Herrero-Uribe, Libia hepatitis a virus negative staining scanning electron microscopy thin sections ultrastructure Previous electron microscope studies of ultrastructural events during hepatitis A virus replication in experimentally infected cells have used only ultrathin section techniques. Nevertheless, no important differences were observed between infected and uninfected cells. This study was carried out using scanning electron microscopy and negative staining of whole LLC-MK2 cells grown directly on grids covered with support membranes, and then infected with an hepatitis A virus strain. Thin sections of infected and unifected controls were also analyzed. An intricate web of projections forming a net between cell interfaces was observed only in infected cells. Some of these projections were more than 700 nm long and had ballooning tips. Nevertheless, HAV particles were not visualized in the infected cells. Los estudios previos sobre los eventos ultraestructuralesen la replicación del virus de la hepatitis Aen cultivos decélulas infectadas experimentalmente, se han realizado exclusivamentemediante cortes ultrafinos. No obstante, no sehan observado diferencias importantes entre células infectadasy no infectadas. Este estudio fue realizado empleandomicroscopia electrónica de rastreo, tinción negativa de células(LLC-MK2) cultivadas directamente sobre rejillas paramicroscopia electrónica de transmisión e infectadas con elvirus de la hepatitis A. Adicionalmente se analizaron cortesultrafinos de células infectadas y no infectadas. Se observóuna intrincada red de proyecciones entre las superficies delas células, que apareció solo en las células infectadas. Algunasde esas proyecciones medían más de 700 nm de largoy terminaban en extremos abultados. Sin embargo, no seobservaron viriones en las células infectadas. Universidad de Costa Rica 2002-06-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article application/pdf https://revistas.ucr.ac.cr/index.php/rbt/article/view/16437 Revista de Biología Tropical; Vol. 50 No. 2 (2002): Volume 50 - Regular number 2 - June 2002; 519–523 Revista de Biología Tropical; Vol. 50 Núm. 2 (2002): Volumen 50 - Número regular 2 - Junio 2002; 519–523 Revista Biología Tropical; Vol. 50 N.º 2 (2002): Volume 50 - Regular number 2 - June 2002; 519–523 2215-2075 0034-7744 eng https://revistas.ucr.ac.cr/index.php/rbt/article/view/16437/15952 Copyright (c) 2002 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0
institution Universidad de Costa Rica
collection Revista de Biología Tropical
language eng
format Online
author Hernández-Chavarría, Francisco
Alzamora-González, Libertad
Herrero-Uribe, Libia
spellingShingle Hernández-Chavarría, Francisco
Alzamora-González, Libertad
Herrero-Uribe, Libia
Noncytopathic hepatitis A virus induces surface alterations in LLC-MK2 cells revealed by thin sections, negative staining, and scanning electron microscopy
author_facet Hernández-Chavarría, Francisco
Alzamora-González, Libertad
Herrero-Uribe, Libia
author_sort Hernández-Chavarría, Francisco
description Los estudios previos sobre los eventos ultraestructuralesen la replicación del virus de la hepatitis Aen cultivos decélulas infectadas experimentalmente, se han realizado exclusivamentemediante cortes ultrafinos. No obstante, no sehan observado diferencias importantes entre células infectadasy no infectadas. Este estudio fue realizado empleandomicroscopia electrónica de rastreo, tinción negativa de células(LLC-MK2) cultivadas directamente sobre rejillas paramicroscopia electrónica de transmisión e infectadas con elvirus de la hepatitis A. Adicionalmente se analizaron cortesultrafinos de células infectadas y no infectadas. Se observóuna intrincada red de proyecciones entre las superficies delas células, que apareció solo en las células infectadas. Algunasde esas proyecciones medían más de 700 nm de largoy terminaban en extremos abultados. Sin embargo, no seobservaron viriones en las células infectadas.
title Noncytopathic hepatitis A virus induces surface alterations in LLC-MK2 cells revealed by thin sections, negative staining, and scanning electron microscopy
title_short Noncytopathic hepatitis A virus induces surface alterations in LLC-MK2 cells revealed by thin sections, negative staining, and scanning electron microscopy
title_full Noncytopathic hepatitis A virus induces surface alterations in LLC-MK2 cells revealed by thin sections, negative staining, and scanning electron microscopy
title_fullStr Noncytopathic hepatitis A virus induces surface alterations in LLC-MK2 cells revealed by thin sections, negative staining, and scanning electron microscopy
title_full_unstemmed Noncytopathic hepatitis A virus induces surface alterations in LLC-MK2 cells revealed by thin sections, negative staining, and scanning electron microscopy
title_sort noncytopathic hepatitis a virus induces surface alterations in llc-mk2 cells revealed by thin sections, negative staining, and scanning electron microscopy
title_alt Noncytopathic hepatitis A virus induces surface alterations in LLC-MK2 cells revealed by thin sections, negative staining, and scanning electron microscopy
publisher Universidad de Costa Rica
publishDate 2002
url https://revistas.ucr.ac.cr/index.php/rbt/article/view/16437
work_keys_str_mv AT hernandezchavarriafrancisco noncytopathichepatitisavirusinducessurfacealterationsinllcmk2cellsrevealedbythinsectionsnegativestainingandscanningelectronmicroscopy
AT alzamoragonzalezlibertad noncytopathichepatitisavirusinducessurfacealterationsinllcmk2cellsrevealedbythinsectionsnegativestainingandscanningelectronmicroscopy
AT herrerouribelibia noncytopathichepatitisavirusinducessurfacealterationsinllcmk2cellsrevealedbythinsectionsnegativestainingandscanningelectronmicroscopy
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