Physicochemical characterization of the biomass of the pineapple stubble (Ananas comosus) and its assessment as a substrate for he mycelial propagation of shiitake (Lentinula edodes) mushroom

Physicochemical characterization of the biomass of the pineapple stubble (Ananas comosus) and its assessment as a substrate for he mycelial propagation of shiitake (Lentinula edodes) mushroom

Bibliographic Details
Main Authors: Vargas, Maynor Vargas, Chaverri, Rodolfo Hernández, Silva, Arlette Jiménez
Format: Online
Language:spa
Published: Universidad Técnica Nacional (Costa Rica) 2019
Online Access:https://revistas.utn.ac.cr/index.php/yulok/article/view/191
id YULOK191
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spelling YULOK1912023-05-30T13:34:10Z Physicochemical characterization of the biomass of the pineapple stubble (Ananas comosus) and its assessment as a substrate for he mycelial propagation of shiitake (Lentinula edodes) mushroom Caracterización de la biomasa de piña (Ananas comosus) y su valoración en la propagación micelial del hongo shiitake (Leninula edodes) Vargas, Maynor Vargas Chaverri, Rodolfo Hernández Silva, Arlette Jiménez biomasa lignocelulósica crecimiento micelial hongo shiitake hidrólisis química rastrojo de piña lignocellulosic biomass pineapple stubble chemical hydrolysis shiitake mushroom mycelial growth The lignocellulosic biomass residues generated in the pineapple cultivation of the Northern Huetar Region were physicochemicallycharacterized in order to evaluate its potential as a culture medium for the mycelial growth of shiitake mushroom(Lentinula edodes). Dry matter contents varied between 11.4 and 28.2% and ash content between 2.96 and 6.44%, ona dry basis. The proportion of water-soluble material was 53.7% and extracts showed a pH of 4.05 ± 0.42, for an acidity of3,48% expressed as lactic acid. The content of reducing sugars was 6.6 ± 1.3% for the leaf biomass, although much lowerin the root system. Cobalt, copper, nickel, zinc, magnesium, potassium, phosphorus and calcium were also determined.In total, nine plant pigments were isolated by extraction with acetone and characterized by visible spectrophotometry.Acid hydrolysis led to a maximum production of free amino acids at pH 3 and 20°C, while the generation of aldehydesunder those conditions was little significant; hence, no antagonistic effects are expected for the mycelial growth underthe extraction conditions. Preliminary microbiological assays showed a remarkable ability of the extracts to supportthe mycelial growth in vitro of shiitake mushroom. Se caracterizaron fisicoquímicamente los residuos de biomasa lignocelulósica de la producción piñera de la RegiónHuetar Norte y se evaluó su potencial como medio de cultivo para la etapa de crecimiento micelial del hongo shiitake(Lentinula edodes). La materia seca, según parte de la planta, varió entre 11,4 y 28,2 % y la ceniza, en base seca, entre2,96 y 6,44%. La proporción hidrosoluble fue de 53,7%; los extractos mostraron un pH de 4,05 ± 0,42 y una acidezde 3,48%, expresada como ácido láctico. El contenido de azúcares reductores fue de 6,6 ± 1,3 % en la biomasa foliar,aunque menor en el sistema radical. Asimismo, se determinó cobalto, cobre, níquel, zinc, magnesio, potasio, fósforoy calcio, nutrientes esenciales para el desarrollo fúngico y se aislaron nueve pigmentos vegetales por extracción conacetona, los cuales fueron caracterizados por espectrofotometría visible. La hidrólisis ácida condujo a una producciónmáxima de aminoácidos libres a pH 3 y 20° C, mientras que la generación de aldehídos fue poco significativa segúnestas condiciones, por lo que no se esperan efectos antagonistas al crecimiento micelial. Ensayos microbiológicos preliminaresdemostraron una notable capacidad de los extractos para soportar el crecimiento micelial in vitro del hongoshiitake. Universidad Técnica Nacional (Costa Rica) 2019-07-16 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.utn.ac.cr/index.php/yulok/article/view/191 10.47633/yulk.v3i1 (enero a junio).191 Yulök Revista de Innovación Académica; Vol. 3 Núm. 1 (2019): Yulök Revista de Innovación Académica (enero - junio); 13-27 2215-5147 2215-5066 spa https://revistas.utn.ac.cr/index.php/yulok/article/view/191/142 Derechos de autor 2019 Maynor Vargas Vargas, Rodolfo Hernández Chaverri, Arlette Jiménez Silva
institution Universidad Técnica Nacional
collection Yulök Revista de Innovación Académica
language spa
format Online
author Vargas, Maynor Vargas
Chaverri, Rodolfo Hernández
Silva, Arlette Jiménez
spellingShingle Vargas, Maynor Vargas
Chaverri, Rodolfo Hernández
Silva, Arlette Jiménez
Physicochemical characterization of the biomass of the pineapple stubble (Ananas comosus) and its assessment as a substrate for he mycelial propagation of shiitake (Lentinula edodes) mushroom
author_facet Vargas, Maynor Vargas
Chaverri, Rodolfo Hernández
Silva, Arlette Jiménez
author_sort Vargas, Maynor Vargas
title Physicochemical characterization of the biomass of the pineapple stubble (Ananas comosus) and its assessment as a substrate for he mycelial propagation of shiitake (Lentinula edodes) mushroom
title_short Physicochemical characterization of the biomass of the pineapple stubble (Ananas comosus) and its assessment as a substrate for he mycelial propagation of shiitake (Lentinula edodes) mushroom
title_full Physicochemical characterization of the biomass of the pineapple stubble (Ananas comosus) and its assessment as a substrate for he mycelial propagation of shiitake (Lentinula edodes) mushroom
title_fullStr Physicochemical characterization of the biomass of the pineapple stubble (Ananas comosus) and its assessment as a substrate for he mycelial propagation of shiitake (Lentinula edodes) mushroom
title_full_unstemmed Physicochemical characterization of the biomass of the pineapple stubble (Ananas comosus) and its assessment as a substrate for he mycelial propagation of shiitake (Lentinula edodes) mushroom
title_sort physicochemical characterization of the biomass of the pineapple stubble (ananas comosus) and its assessment as a substrate for he mycelial propagation of shiitake (lentinula edodes) mushroom
title_alt Caracterización de la biomasa de piña (Ananas comosus) y su valoración en la propagación micelial del hongo shiitake (Leninula edodes)
publisher Universidad Técnica Nacional (Costa Rica)
publishDate 2019
url https://revistas.utn.ac.cr/index.php/yulok/article/view/191
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AT silvaarlettejimenez physicochemicalcharacterizationofthebiomassofthepineapplestubbleananascomosusanditsassessmentasasubstrateforhemycelialpropagationofshiitakelentinulaedodesmushroom
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