Diagnosis of Black Queen Cell Virus, Deformed Wing Virus and Acute Bee Paralysis Virus in symptomatic African honey bees (Apis mellifera scutellata) of Costa Rica using RT-PCR in real time.

Detalles Bibliográficos
Autores principales:	Chaves Guevara, Guisella, Cubero Murillo, Ana, Cordero, Juan Miguel, Vicente-Rubiano, Marina, Kukielka, Deborah, León, Bernal, Sánchez-Vizcaíno, José Manuel
Formato:	Online
Idioma:	spa
Publicado:	Universidad Nacional, Costa Rica 2016
Acceso en línea:	https://www.revistas.una.ac.cr/index.php/veterinaria/article/view/8487

id	VETERINARIA8487
record_format	ojs
spelling	VETERINARIA84872019-08-27T15:19:54Z Diagnosis of Black Queen Cell Virus, Deformed Wing Virus and Acute Bee Paralysis Virus in symptomatic African honey bees (Apis mellifera scutellata) of Costa Rica using RT-PCR in real time. Diagnóstico del Virus de la Celda Real Negra, el Virus de Alas Deformes y el Virus de la Parálisis Aguda en abejas africanizadas (Apis mellifera scutellata), sintomáticas de Costa Rica, utilizando RT‐PCR en tiempo real. Chaves Guevara, Guisella Cubero Murillo, Ana Cordero, Juan Miguel Vicente-Rubiano, Marina Kukielka, Deborah León, Bernal Sánchez-Vizcaíno, José Manuel Bee Apis mellifera scutellata RT-PCR real time SYBR Green DWV BQCV ABPV Costa Rica Abejas Apis mellifera scutellata RT-PCR tiempo real SYBR Green DWV BQCV ABPV Costa Rica After beekeeper claims of bee diseases in beehives located in the South central region of the province of San José, Costa Rica, samples were collected from live adult Apis mellifera scutellata bees having presumptive symptoms of viral infection. Samples were extracted and processed in the laboratory. This is the first diagnostic in Costa Rica of the presence of the Black Queen Cell Virus (BQCV) and the Acute Bee Paralysis Virus (ABPV) in the province of San José in the canton of Turrubares, the district of San Pedro and the canton of Tarrazú, the district of San Marcos, respectively. Furthermore, this is also the second time that the Deformed Wing Virus DWV was detected in diseased bees in the country, in this occasion using PCR. Information about viral diseases in our country is still scarce; therefore, the implementation of these methods opens the possibility to offer beekeepers a rapid, specific and sensitive diagnosis. The development of these techniques could also be the starting point for conducting research to determine the prevalence of these viruses in beehives as well as potential risk factors associated with these infections. Tras denuncias de apicultores, por la presencia de abejas Apis mellifera scutellata enfermas en colmenas localizadas en la región central sur de la provincia de San José, Costa Rica, se recolectó muestras de abejas adultas vivas, con sintomatología presuntiva de infección viral. Las muestras fueron extraídas y procesadas en el laboratorio. A partir de estas muestras, se diagnosticó, por primera vez en Costa Rica, la presencia del virus de la Celda Real Negra (BQCV) y el virus de la Parálisis Aguda (ABPV), en los cantones de: Turrubares, distrito San Pedro; Tarrazú, distrito San Marcos, respectivamente. Además, se confirmó, por PCR, la presencia del virus de las Alas Deformes (DWV) en el país. La información, acerca de las enfermedades virales en Costa Rica, es todavía escasa, por lo cual, la implementación de estas metodologías abre la posibilidad de ofrecer, a los apicultores de este país, un diagnóstico rápido, específico y sensible. La puesta a punto de estas técnicas, además, podría ser el inicio para la realización de investigaciones con el fin de determinar la prevalencia de virus en las colmenas costarricenses, así como posibles factores de riesgo asociados a estas infecciones. Universidad Nacional, Costa Rica 2016-07-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html https://www.revistas.una.ac.cr/index.php/veterinaria/article/view/8487 10.15359/rcv.34-1.1 Ciencias Veterinarias; Vol. 34 No. 1 (2016): Ciencias Veterinarias (Enero-Junio); 7-21 Ciencias Veterinarias; Vol. 34 Núm. 1 (2016): Ciencias Veterinarias (Enero-Junio); 7-21 2215-4507 0250-5649 spa https://www.revistas.una.ac.cr/index.php/veterinaria/article/view/8487/9606 https://www.revistas.una.ac.cr/index.php/veterinaria/article/view/8487/9704 Copyright (c) 2016 Revista Ciencias Veterinarias
institution	Universidad Nacional de Costa Rica
collection	Ciencias Veterinarias
language	spa
format	Online
author	Chaves Guevara, Guisella Cubero Murillo, Ana Cordero, Juan Miguel Vicente-Rubiano, Marina Kukielka, Deborah León, Bernal Sánchez-Vizcaíno, José Manuel
spellingShingle	Chaves Guevara, Guisella Cubero Murillo, Ana Cordero, Juan Miguel Vicente-Rubiano, Marina Kukielka, Deborah León, Bernal Sánchez-Vizcaíno, José Manuel Diagnosis of Black Queen Cell Virus, Deformed Wing Virus and Acute Bee Paralysis Virus in symptomatic African honey bees (Apis mellifera scutellata) of Costa Rica using RT-PCR in real time.
author_facet	Chaves Guevara, Guisella Cubero Murillo, Ana Cordero, Juan Miguel Vicente-Rubiano, Marina Kukielka, Deborah León, Bernal Sánchez-Vizcaíno, José Manuel
author_sort	Chaves Guevara, Guisella
title	Diagnosis of Black Queen Cell Virus, Deformed Wing Virus and Acute Bee Paralysis Virus in symptomatic African honey bees (Apis mellifera scutellata) of Costa Rica using RT-PCR in real time.
title_short	Diagnosis of Black Queen Cell Virus, Deformed Wing Virus and Acute Bee Paralysis Virus in symptomatic African honey bees (Apis mellifera scutellata) of Costa Rica using RT-PCR in real time.
title_full	Diagnosis of Black Queen Cell Virus, Deformed Wing Virus and Acute Bee Paralysis Virus in symptomatic African honey bees (Apis mellifera scutellata) of Costa Rica using RT-PCR in real time.
title_fullStr	Diagnosis of Black Queen Cell Virus, Deformed Wing Virus and Acute Bee Paralysis Virus in symptomatic African honey bees (Apis mellifera scutellata) of Costa Rica using RT-PCR in real time.
title_full_unstemmed	Diagnosis of Black Queen Cell Virus, Deformed Wing Virus and Acute Bee Paralysis Virus in symptomatic African honey bees (Apis mellifera scutellata) of Costa Rica using RT-PCR in real time.
title_sort	diagnosis of black queen cell virus, deformed wing virus and acute bee paralysis virus in symptomatic african honey bees (apis mellifera scutellata) of costa rica using rt-pcr in real time.
title_alt	Diagnóstico del Virus de la Celda Real Negra, el Virus de Alas Deformes y el Virus de la Parálisis Aguda en abejas africanizadas (Apis mellifera scutellata), sintomáticas de Costa Rica, utilizando RT‐PCR en tiempo real.
publisher	Universidad Nacional, Costa Rica
publishDate	2016
url	https://www.revistas.una.ac.cr/index.php/veterinaria/article/view/8487
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Diagnosis of Black Queen Cell Virus, Deformed Wing Virus and Acute Bee Paralysis Virus in symptomatic African honey bees (Apis mellifera scutellata) of Costa Rica using RT-PCR in real time.

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