Comparison of competitive enzyme-linked immunosorbent assay and serum neutralization test for the detection of antibodies to vesicular stomatitis virus (New Jersey and Indiana serotypes) in equines from Costa Rica
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| Formato: | Online |
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Universidad Nacional, Costa Rica
2015
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| Acceso en línea: | https://www.revistas.una.ac.cr/index.php/veterinaria/article/view/6963 |
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VETERINARIA6963 |
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VETERINARIA69632020-02-03T05:56:34Z Comparison of competitive enzyme-linked immunosorbent assay and serum neutralization test for the detection of antibodies to vesicular stomatitis virus (New Jersey and Indiana serotypes) in equines from Costa Rica Comparación de la técnica seroneutralización con la inmunoenzimática competitiva en la detección de anticuerpos contra el virus de la estomatitis vesicular (serotipos New Jersey e Indiana) en equinos de Costa Rica von Koeckritz-Blickwede, Maren Dolz Wiedner, Gaby Vinicio Herrero, Marco A competitive enzyme-linked immunosorbent assay (c-ELISA) was compared to the serum neutralization test (SNT) for the detection of antibodies against vesicular stomatitis virus, New Jersey (VSV-NJ) and Indiana (VSV-IN) serotypes, using 214 equine serum samples from Costa Rica. In the c-ELISA for VSV-NJ 109 (50.93%) equine sera reacted positive, whereas in the SNT 138 (64.48%) serum samples reacted positive to VSV-NJ. In the c-ELISA for VSV-IN 26 (12.15%) sera reacted positive, whereas in the SNT 36 (16.83%) equine sera reacted positive to VSV-IN. The c-ELISA showed a moderate sensitivity compared to the SNT. The rapidity and ease of the c-ELISA system makes it suitable as a rapid diagnostic screening assay. However, because of the lower sensitivity of the c-ELISA, the SNT is considered the method of choice to detect equine VSV infections in Costa Rica. En este trabajo se comparó la técnica de seroneutralización (SNT) con un ensayo inmunoenzimático competitivo (c-ELISA) para la detección de anticuerpos contra el virus de la estomatitis vesicular, serotipos New Jersey (VSV-NJ) e Indiana (VSV-IN), utilizando 214 sueros equinos de Costa Rica. El c-ELISA detectó 109 (50.93%) sueros positivos; mientras que en SNT se determinó un total de 138 (64,48%) animales positivos a VSV-NJ. Un total de 26 (12.15%) sueros resultó positivos en c-ELISA; mientras que 36 (16,83%) sueros se determinó como positivos en SNT para VSV-IN. La técnica inmunoenzimática mostró una moderada sensibilidad, en comparación con la técnica de seroneutralización. Por su rapidez y fácil ejecución, el ELISA competitivo resulta una técnica importante de tamizaje; sin embargo, debido a la menor sensibilidad del c-ELISA, se considera la SNT como el método de elección para detectar infecciones equinas con VSV en Costa Rica. Universidad Nacional, Costa Rica 2015-09-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://www.revistas.una.ac.cr/index.php/veterinaria/article/view/6963 Ciencias Veterinarias; Vol 32 No 1 (2014): Ciencias Veterinarias (Enero-Junio); 21-27 Ciencias Veterinarias; Vol. 32 Núm. 1 (2014): Ciencias Veterinarias (Enero-Junio); 21-27 2215-4507 0250-5649 spa https://www.revistas.una.ac.cr/index.php/veterinaria/article/view/6963/7190 |
| institution |
Universidad Nacional de Costa Rica |
| collection |
Ciencias Veterinarias |
| language |
spa |
| format |
Online |
| author |
von Koeckritz-Blickwede, Maren Dolz Wiedner, Gaby Vinicio Herrero, Marco |
| spellingShingle |
von Koeckritz-Blickwede, Maren Dolz Wiedner, Gaby Vinicio Herrero, Marco Comparison of competitive enzyme-linked immunosorbent assay and serum neutralization test for the detection of antibodies to vesicular stomatitis virus (New Jersey and Indiana serotypes) in equines from Costa Rica |
| author_facet |
von Koeckritz-Blickwede, Maren Dolz Wiedner, Gaby Vinicio Herrero, Marco |
| author_sort |
von Koeckritz-Blickwede, Maren |
| title |
Comparison of competitive enzyme-linked immunosorbent assay and serum neutralization test for the detection of antibodies to vesicular stomatitis virus (New Jersey and Indiana serotypes) in equines from Costa Rica |
| title_short |
Comparison of competitive enzyme-linked immunosorbent assay and serum neutralization test for the detection of antibodies to vesicular stomatitis virus (New Jersey and Indiana serotypes) in equines from Costa Rica |
| title_full |
Comparison of competitive enzyme-linked immunosorbent assay and serum neutralization test for the detection of antibodies to vesicular stomatitis virus (New Jersey and Indiana serotypes) in equines from Costa Rica |
| title_fullStr |
Comparison of competitive enzyme-linked immunosorbent assay and serum neutralization test for the detection of antibodies to vesicular stomatitis virus (New Jersey and Indiana serotypes) in equines from Costa Rica |
| title_full_unstemmed |
Comparison of competitive enzyme-linked immunosorbent assay and serum neutralization test for the detection of antibodies to vesicular stomatitis virus (New Jersey and Indiana serotypes) in equines from Costa Rica |
| title_sort |
comparison of competitive enzyme-linked immunosorbent assay and serum neutralization test for the detection of antibodies to vesicular stomatitis virus (new jersey and indiana serotypes) in equines from costa rica |
| title_alt |
Comparación de la técnica seroneutralización con la inmunoenzimática competitiva en la detección de anticuerpos contra el virus de la estomatitis vesicular (serotipos New Jersey e Indiana) en equinos de Costa Rica |
| publisher |
Universidad Nacional, Costa Rica |
| publishDate |
2015 |
| url |
https://www.revistas.una.ac.cr/index.php/veterinaria/article/view/6963 |
| work_keys_str_mv |
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