Serological relationships between rice Hoja Blanca Virus AND Echinochloa Hoja Blanca Virus (ESP)
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| Formato: | Online |
| Idioma: | eng spa |
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Universidad Nacional, Costa Rica
2000
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| Acceso en línea: | https://www.revistas.una.ac.cr/index.php/uniciencia/article/view/5701 |
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UNICIENCIA5701 |
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UNICIENCIA57012018-08-08T23:35:16Z Serological relationships between rice Hoja Blanca Virus AND Echinochloa Hoja Blanca Virus (ESP) Serological relationships between rice Hoja Blanca Virus AND Echinochloa Hoja Blanca Virus Madriz, Jorge Bonilla, José R. De Miranda, Joachim Espinoza, Ana With the objective of determining whether rice hoja blanca virus (RHBV) and Echinochloa hoja blanca virus (EHBV) are serologically related, antisera were produced against the ribonucleoproteins of both viruses and used in several comparative studies. It was demonstrated that both viruses possess characteristic that separate them at this level. When compared in high resolution SDS-PAGE gels, it was observed that the EHBV nucleoprotein had a lower molecular mass than that of RHBV. The antiserum against RHBV recognizes the EHBV nucleoprotein in Western blot, indicating the presence of common epitope. Reciprocal double immunodiffusion (OUCHTERLONY) tests showed the presence of both common and unique epitopes of the nucleoproteins of both viruses. Con el objetivo de determinar si el RHBV (Rice Hoja Blanca Virus) y el EHBV (Echinochloa Hoja Blanca Virus) se hallan relacionados serológicamente, se produjo un antisuero contra la proteína de nucleocápside del EHBV. Se demostró que ambos virus presentan características que los diferencian a este nivel. Al comparar la proteína de nucleocápside en geles SDS-PAGE de alta resolución, se observó que dicha proteína en el EHBV presenta una masa molecular menor que la del RHBV. El antisuero contra la proteína N del RHBV producido por Espinoza et al. (1992) reconoce la del EHBV por Western blot. Lo anterior indica que son similares y sugiere la presencia de epitopos comunes; además confirma que el EHBV presenta una menor masa molecular que el RHBV. Asimismo, el método de inmunodifusión doble de OUCHTERLONY mostró una identidad parcial de las nucleoproteínas de ambos virus, lo que confirma que ambos virus se encuentran serológicamente relacionados. Universidad Nacional, Costa Rica 2000-01-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Papers evaluated by academic peers Artículos evaluados por pares académicos application/pdf application/pdf https://www.revistas.una.ac.cr/index.php/uniciencia/article/view/5701 Uniciencia; Vol. 17 No. 1 (2000); 21-26 Uniciencia; Vol. 17 Núm. 1 (2000); 21-26 Uniciencia; v. 17 n. 1 (2000); 21-26 2215-3470 eng spa https://www.revistas.una.ac.cr/index.php/uniciencia/article/view/5701/5571 https://www.revistas.una.ac.cr/index.php/uniciencia/article/view/5701/6502 |
| institution |
Universidad Nacional de Costa Rica |
| collection |
Uniciencia |
| language |
eng spa |
| format |
Online |
| author |
Madriz, Jorge Bonilla, José R. De Miranda, Joachim Espinoza, Ana |
| spellingShingle |
Madriz, Jorge Bonilla, José R. De Miranda, Joachim Espinoza, Ana Serological relationships between rice Hoja Blanca Virus AND Echinochloa Hoja Blanca Virus (ESP) |
| author_facet |
Madriz, Jorge Bonilla, José R. De Miranda, Joachim Espinoza, Ana |
| author_sort |
Madriz, Jorge |
| title |
Serological relationships between rice Hoja Blanca Virus AND Echinochloa Hoja Blanca Virus (ESP) |
| title_short |
Serological relationships between rice Hoja Blanca Virus AND Echinochloa Hoja Blanca Virus (ESP) |
| title_full |
Serological relationships between rice Hoja Blanca Virus AND Echinochloa Hoja Blanca Virus (ESP) |
| title_fullStr |
Serological relationships between rice Hoja Blanca Virus AND Echinochloa Hoja Blanca Virus (ESP) |
| title_full_unstemmed |
Serological relationships between rice Hoja Blanca Virus AND Echinochloa Hoja Blanca Virus (ESP) |
| title_sort |
serological relationships between rice hoja blanca virus and echinochloa hoja blanca virus (esp) |
| title_alt |
Serological relationships between rice Hoja Blanca Virus AND Echinochloa Hoja Blanca Virus |
| publisher |
Universidad Nacional, Costa Rica |
| publishDate |
2000 |
| url |
https://www.revistas.una.ac.cr/index.php/uniciencia/article/view/5701 |
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