Study on the etiology of fibropapillomatosis of olive ridley sea turtles (Lepidochelys olivacea) nesting in the National Wildlife Refuge at Ostional, Guanacaste, Costa Rica
| Autores principales: | , , , , |
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| Formato: | Online |
| Idioma: | spa |
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Universidad Nacional, Costa Rica.
2013
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| Acceso en línea: | https://www.revistas.una.ac.cr/index.php/revmar/article/view/5557 |
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REVMAR5557 |
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ojs |
| institution |
Universidad Nacional de Costa Rica |
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Revista Ciencias Marinas y Costeras |
| language |
spa |
| format |
Online |
| author |
Brenes-Chaves, Laura Berrocal, Alexis Meneses, Ana I. Jiménez-Sánchez, Carlos Orrego-Vásquez, Carlos M. |
| spellingShingle |
Brenes-Chaves, Laura Berrocal, Alexis Meneses, Ana I. Jiménez-Sánchez, Carlos Orrego-Vásquez, Carlos M. Study on the etiology of fibropapillomatosis of olive ridley sea turtles (Lepidochelys olivacea) nesting in the National Wildlife Refuge at Ostional, Guanacaste, Costa Rica |
| author_facet |
Brenes-Chaves, Laura Berrocal, Alexis Meneses, Ana I. Jiménez-Sánchez, Carlos Orrego-Vásquez, Carlos M. |
| author_sort |
Brenes-Chaves, Laura |
| title |
Study on the etiology of fibropapillomatosis of olive ridley sea turtles (Lepidochelys olivacea) nesting in the National Wildlife Refuge at Ostional, Guanacaste, Costa Rica |
| title_short |
Study on the etiology of fibropapillomatosis of olive ridley sea turtles (Lepidochelys olivacea) nesting in the National Wildlife Refuge at Ostional, Guanacaste, Costa Rica |
| title_full |
Study on the etiology of fibropapillomatosis of olive ridley sea turtles (Lepidochelys olivacea) nesting in the National Wildlife Refuge at Ostional, Guanacaste, Costa Rica |
| title_fullStr |
Study on the etiology of fibropapillomatosis of olive ridley sea turtles (Lepidochelys olivacea) nesting in the National Wildlife Refuge at Ostional, Guanacaste, Costa Rica |
| title_full_unstemmed |
Study on the etiology of fibropapillomatosis of olive ridley sea turtles (Lepidochelys olivacea) nesting in the National Wildlife Refuge at Ostional, Guanacaste, Costa Rica |
| title_sort |
study on the etiology of fibropapillomatosis of olive ridley sea turtles (lepidochelys olivacea) nesting in the national wildlife refuge at ostional, guanacaste, costa rica |
| title_alt |
Estudio sobre la etiología de la fibropapilomatosis de tortugas litorales ( Lepidochelys olivacea ) que anidan en el Refugio Nacional de Vida Silvestre en Ostional, Guanacaste, Costa Rica Estudo da etiologia da fibropapilomatose da tartaruga-oliva (Lepidochelys olivacea) que aninha no Refúgio Nacional de Vida Silvestre de Ostional, Guanacaste, Costa Rica |
| publisher |
Universidad Nacional, Costa Rica. |
| publishDate |
2013 |
| url |
https://www.revistas.una.ac.cr/index.php/revmar/article/view/5557 |
| work_keys_str_mv |
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1805401720981815296 |
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REVMAR55572022-06-02T22:25:06Z Study on the etiology of fibropapillomatosis of olive ridley sea turtles (Lepidochelys olivacea) nesting in the National Wildlife Refuge at Ostional, Guanacaste, Costa Rica Estudio sobre la etiología de la fibropapilomatosis de tortugas litorales ( Lepidochelys olivacea ) que anidan en el Refugio Nacional de Vida Silvestre en Ostional, Guanacaste, Costa Rica Estudo da etiologia da fibropapilomatose da tartaruga-oliva (Lepidochelys olivacea) que aninha no Refúgio Nacional de Vida Silvestre de Ostional, Guanacaste, Costa Rica Brenes-Chaves, Laura Berrocal, Alexis Meneses, Ana I. Jiménez-Sánchez, Carlos Orrego-Vásquez, Carlos M. Lepidochelys olivacea fibropapilloma PCR herpesvirus Lepidochelys olivacea fibropapilloma PCR herpesvirus fibropapiloma Lepidochelys olivacea fibropapiloma PCR herpesvírus. Sea turtle fibropapillomatosis is an emerging disease characterized by a proliferation of cutaneous papillomas, fibromas, and fibropapillomas and occasional visceral fibromas. This paper aims to contribute to the etiology of fibropapillomatosis in olive ridley sea turtles (Lepidochelys olivacea) nesting in Ostional National Wildlife Refuge. Twenty-six olive ridley turtles with cutaneous fibropapilloma were sampled and 24 healthy olive ridley turtles served as controls. Biopsies were taken of the cutaneous tumors in sick sea turtles, as well as skin biopsies from control subjects, and blood samples were collected from all turtles. Tumorous samples and skin samples were microscopically analyzed in order to differentiate the histological factors resulting from the disease pathogenesis, where the main histological findings were papillary epidermal hyperplasia, orthokeratotic hyperkeratosis, spirorchid-like eggs in the dermis, and eosinophilic cytoplasmic inclusion. Hematology and blood chemistry studies were conducted on blood samples, and MCHC, heterophils, lymphocytes, monocytes, AST, total protein, albumin and globulin values were significantly different between healthy turtles and turtles with tumors. A PCR test was also conducted in the samples to determine the presence of herpesvirus and papillomavirus as possible etiologic agents, where the papilomavirus was absent in all the samples, while the herpesvirus was present in 69.23% of the tumors, this being the most probable etiological agent of fibropapillomatosis. La fibropapilomatosis de la tortuga marina es una enfermedad emergente caracterizada por múltiples papilomas, fibromas y fibropapilomas cutáneos, así como ocasionales fibromas viscerales. El presente trabajo tiene como objetivo contribuir a la etiología de la fibropapilomatosis en la tortuga lora (Lepidochelys olivacea) que anida en el Refugio Nacional de Vida Silvestre Ostional. Se muestrearon 26 tortugas lora con fibropapilomas cutáneos y 24 tortugas lora sanas que sirvieron de control. Se tomaron biopsias excisionales de los tumores cutáneos de las tortugas enfermas y biopsias de piel de las tortugas control, además se recolectaron muestras de sangre de todas las tortugas. Las muestras tumorales y de piel se analizaron microscópicamente para diferenciar los factores histológicos que resultan de la patogénesis de la enfermedad, donde los hallazgos histopatológicos principales en los fibropapilomas fueron: crecimiento papiliforme, hiperqueratosis ortoqueratótica, huevos de parásitos similares a espiróquidos en la capa dérmica e inclusión eosinofílica citoplasmática. A las muestras de sangre se les realizó análisis hematológico y de química sanguínea, donde los valores de CHCM, heterófilos, linfocitos, monocitos, AST, proteínas totales, albúmina y globulinas resultaron significativamente diferentes entre las tortugas sanas y las tortugas con tumores. Se realizó PCR a las muestras para determinar la presencia de genoma de herpesvirus y papilomavirus como posibles agentes etiológicos, donde el papilomavirus estuvo ausente en la totalidad de las muestras, mientras el virus Herpes se presentó en el 69.23% de los tumores posicionándose como el posible agente etiológico de la enfermedad. A fibropapilomatose da tartaruga marinha é uma enfermidade emergente caracterizada por múltiplos papilomas, fibromas e fibropapilomas cutâneos, assim como ocasionais fibromas viscerais. O presente trabalho tem como objetivo contribuir na etiologia da fibropapilomatose na tartaruga-oliva (Lepidochelys olivacea) que aninha no Refúgio Nacional de Vida Silvestre Ostional. Foram amostradas 26 tartarugas-oliva com fibropapilomas cutâneos e 24 tartarugas-oliva saudáveis que serviram de controle. Foram feitas biopsias excisionais dos tumores cutâneos das tartarugas doentes e biopsias de pele das tartarugas controladas, além disso, foram colhidas amostras de sangre de todas as tartarugas. As amostras tumorais e de pele foram analisadas microscopicamente para diferenciar os fatores histológicos resultantes da patogênese da enfermidade, onde os achados histopatológicos principais nos fibropapilomas foram: crescimento papiliforme, hiperqueratose ortoqueratótica, ovos de parasitas similares a espiroquetas na capa dérmica e inclusão eosinofílica citoplasmática. Foi realizada análise hematológica e de química sanguínea nas amostras de sangue onde os valores de CHCM, heterofilos, linfócitos, monócitos, AST, proteínas totais, albumina e globulinas resultaram significativamente diferentes entre as tartarugas saudáveis e as tartarugas com tumores. Foi realizado PCR nas amostras para determinar a presença de genoma de herpesvírus e papilomavírus como possíveis agentes etiológicos, onde o papilomavírus esteve ausente na totalidade das amostras, enquanto o vírus Herpes se apresentou em 69.23% dos tumores posicionando-se como o possível agente etiológico da enfermidade. Universidad Nacional, Costa Rica. 2013-12-10 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed article Artículo evaluado por pares Artigo revisto por pares application/pdf text/html application/epub+zip https://www.revistas.una.ac.cr/index.php/revmar/article/view/5557 10.15359/revmar.5.8 Journal of Marine and Coastal Sciences; Vol. 5 (2013); 119-134 Revista Ciencias Marinas y Costeras; Vol. 5 (2013); 119-134 Revista Ciencias Marinas y Costeras; v. 5 (2013); 119-134 1659-407X 1659-455X spa https://www.revistas.una.ac.cr/index.php/revmar/article/view/5557/5383 https://www.revistas.una.ac.cr/index.php/revmar/article/view/5557/5390 https://www.revistas.una.ac.cr/index.php/revmar/article/view/5557/14746 |