Hardening of the national flower of Colombia, the threatened Cattleya trianae (Orchidaceae), from in vitro culture with previous invigoration phase
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Universidad de Costa Rica
2007
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Acceso en línea: | https://revistas.ucr.ac.cr/index.php/rbt/article/view/6045 |
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RBT60452022-05-30T19:05:50Z Hardening of the national flower of Colombia, the threatened Cattleya trianae (Orchidaceae), from in vitro culture with previous invigoration phase Hardening of the national flower of Colombia, the threatened Cattleya trianae (Orchidaceae), from in vitro culture with previous invigoration phase Franco, Marcela Guevara, Giovany Mesa, Neftalí Urueña, Gloria Aclimatación endurecimiento sustratos ornamentales orquídeas conservación Cattleya trianae Acclimation hardening substrates ornamental orchids conservation Cattleya trianae is an endemic species from the tropical rainforest in the Colombian Andes. Its survival is currently threatened due to habitat loss and commercial overexploitation. This study evaluates ten substrates, some organic (pine bark, coconut fiber and wood shavings), some inert icopor (polystyrene foam), vegetable coal and their combinations, and the effects these have on morphometric and phenotypic traits in the hardening phase of 250 plants of C. trianae cultivated in vitro. Recorded data include percent survival, length of longest leaf, biomass (wet weight) and number of roots and leaves at the beginning and at the end of the experiment. After the hardening phase, the plants were taken to a greenhouse and later to the natural environment. Coconut fiber alone or mixed in equal parts with pine bark and coal was the most efficient substrate when percent survival (80±SE=0.3742), biomass, and leaf length were evaluated. Hardened plants displayed qualitative characteristics such as vigor, hardness and waxy texture, strength of green coloration in the leaves, and velamen formation. Under greenhouse conditions, plants grew better with filtered light, relative humidity bordering on 80 %, permanent aeration, misting with water, and an average temperature of 25±2 °C. Invigorated plants were firmly anchored on their host trees. Universidad de Costa Rica 2007-06-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article Text application/pdf https://revistas.ucr.ac.cr/index.php/rbt/article/view/6045 10.15517/rbt.v55i2.6045 Revista de Biología Tropical; Vol. 55 No. 2 (2007): Volume 55 – Regular number 2 – June 2007; 681–691 Revista de Biología Tropical; Vol. 55 Núm. 2 (2007): Volumen 55 – Número regular 2 – Junio 2007; 681–691 Revista Biología Tropical; Vol. 55 N.º 2 (2007): Volume 55 – Regular number 2 – June 2007; 681–691 2215-2075 0034-7744 10.15517/rbt.v55i2 eng https://revistas.ucr.ac.cr/index.php/rbt/article/view/6045/5750 Copyright (c) 2007 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0 |
institution |
Universidad de Costa Rica |
collection |
Revista de Biología Tropical |
language |
eng |
format |
Online |
author |
Franco, Marcela Guevara, Giovany Mesa, Neftalí Urueña, Gloria |
spellingShingle |
Franco, Marcela Guevara, Giovany Mesa, Neftalí Urueña, Gloria Hardening of the national flower of Colombia, the threatened Cattleya trianae (Orchidaceae), from in vitro culture with previous invigoration phase |
author_facet |
Franco, Marcela Guevara, Giovany Mesa, Neftalí Urueña, Gloria |
author_sort |
Franco, Marcela |
title |
Hardening of the national flower of Colombia, the threatened Cattleya trianae (Orchidaceae), from in vitro culture with previous invigoration phase |
title_short |
Hardening of the national flower of Colombia, the threatened Cattleya trianae (Orchidaceae), from in vitro culture with previous invigoration phase |
title_full |
Hardening of the national flower of Colombia, the threatened Cattleya trianae (Orchidaceae), from in vitro culture with previous invigoration phase |
title_fullStr |
Hardening of the national flower of Colombia, the threatened Cattleya trianae (Orchidaceae), from in vitro culture with previous invigoration phase |
title_full_unstemmed |
Hardening of the national flower of Colombia, the threatened Cattleya trianae (Orchidaceae), from in vitro culture with previous invigoration phase |
title_sort |
hardening of the national flower of colombia, the threatened cattleya trianae (orchidaceae), from in vitro culture with previous invigoration phase |
title_alt |
Hardening of the national flower of Colombia, the threatened Cattleya trianae (Orchidaceae), from in vitro culture with previous invigoration phase |
publisher |
Universidad de Costa Rica |
publishDate |
2007 |
url |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/6045 |
work_keys_str_mv |
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_version_ |
1810114585221398528 |