Molecular phylogenetic relationships among four species of the mangrove tree genus Bruguiera (Rhizophoraceae), as revealed by chromosome and RAPD markers
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Universidad de Costa Rica
2007
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| Acceso en línea: | https://revistas.ucr.ac.cr/index.php/rbt/article/view/6023 |
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RBT60232022-05-30T19:05:50Z Molecular phylogenetic relationships among four species of the mangrove tree genus Bruguiera (Rhizophoraceae), as revealed by chromosome and RAPD markers Molecular phylogenetic relationships among four species of the mangrove tree genus Bruguiera (Rhizophoraceae), as revealed by chromosome and RAPD markers Sahoo, Pragnya Jena, Satyanarayan Mohanty, Suprava Bandhu Das, Anath Contenido de ADN4C Bruguiera filogenia molecular mangle cariotipo 4C DNA content molecular phylogeny tree mangrove karyotype Analysis of karyotype, nuclear DNA content and RAPD markers were performed in four species of Bruguiera (Rhizophoraceae) of Bhitarkanika mangrove forests, Orissa, India. Detailed karyotype analysis revealing 2n=34 in B. cylindrica and 2n=36 in B. gymnorrhiza was reported for the first time and 2n=34 in B. parviflora and B. sexangula was confirmed. On the basis of the common types of chromosomes present among Bruguiera, two distinct groups were found; one consists of B. cylindrica and B. parviflora and the other of B. gymnorrhiza and B. sexangula. The symmetrical karyotype with same chromosome types grouped B. cylindrica and B. parviflora together and presence of Type E chromosomes placed B. gymnorrhiza and B. sexangula in a separate group, suggesting their closer affinity in their respective group. Analysis of chromosome length, volume, INV and 4C DNA content confirmed this division. Nuclear DNA content was two-fold higher (~17.0 pg) in the second group than in the first (~8.0 pg). The amplification products generated through RAPD revealed 1-9 amplicons with size variations from 600 bp to 2 500 bp with 49.31% genetic similarity between B. gymnorrhiza and B. sexangula and 47.10% in between B. cylindrica and B. parviflora. The high copy number marker band (~ 1 100 bp) yielded in OPN-15 primer in B. parviflora the characteristic DNA marker, which was cloned and used as probes for assessment of genetic diversity, and demonstrated its close genetic affinity to B. cylindrica. B. gymnorrhiza and B. sexangula also produced similar marker bands of ~600 bp and ~2 200 bp in the same primer. All of the cytological, 4C DNA content and RAPD data confirmed the existence of two taxonomically distinct groups of Bruguiera: one consisting of B. cylindrica and B. parviflora and the other of B. gymnorrhiza and B. sexangula as placed earlier (1862) in the tribe Rhizophoreae by Bentham and Hooker, on the basis of the flowering habits of Bruguiera. Genetically, the B. sexangula and B. gymnorrhiza group was found to be very closely, rather than distantly, related to B. parviflora and B. cylindrica. Our results demonstrate that molecular markers together with cytological evidence provide an effective tool to access the existing interspecific genetic polymorphism in mangrove species, to solve the taxonomic problems and to design their conservation strategy. Universidad de Costa Rica 2007-06-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article Text application/pdf https://revistas.ucr.ac.cr/index.php/rbt/article/view/6023 10.15517/rbt.v55i2.6023 Revista de Biología Tropical; Vol. 55 No. 2 (2007): Volume 55 – Regular number 2 – June 2007; 437–448 Revista de Biología Tropical; Vol. 55 Núm. 2 (2007): Volumen 55 – Número regular 2 – Junio 2007; 437–448 Revista Biología Tropical; Vol. 55 N.º 2 (2007): Volume 55 – Regular number 2 – June 2007; 437–448 2215-2075 0034-7744 10.15517/rbt.v55i2 eng https://revistas.ucr.ac.cr/index.php/rbt/article/view/6023/5729 Copyright (c) 2007 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0 |
| institution |
Universidad de Costa Rica |
| collection |
Revista de Biología Tropical |
| language |
eng |
| format |
Online |
| author |
Sahoo, Pragnya Jena, Satyanarayan Mohanty, Suprava Bandhu Das, Anath |
| spellingShingle |
Sahoo, Pragnya Jena, Satyanarayan Mohanty, Suprava Bandhu Das, Anath Molecular phylogenetic relationships among four species of the mangrove tree genus Bruguiera (Rhizophoraceae), as revealed by chromosome and RAPD markers |
| author_facet |
Sahoo, Pragnya Jena, Satyanarayan Mohanty, Suprava Bandhu Das, Anath |
| author_sort |
Sahoo, Pragnya |
| title |
Molecular phylogenetic relationships among four species of the mangrove tree genus Bruguiera (Rhizophoraceae), as revealed by chromosome and RAPD markers |
| title_short |
Molecular phylogenetic relationships among four species of the mangrove tree genus Bruguiera (Rhizophoraceae), as revealed by chromosome and RAPD markers |
| title_full |
Molecular phylogenetic relationships among four species of the mangrove tree genus Bruguiera (Rhizophoraceae), as revealed by chromosome and RAPD markers |
| title_fullStr |
Molecular phylogenetic relationships among four species of the mangrove tree genus Bruguiera (Rhizophoraceae), as revealed by chromosome and RAPD markers |
| title_full_unstemmed |
Molecular phylogenetic relationships among four species of the mangrove tree genus Bruguiera (Rhizophoraceae), as revealed by chromosome and RAPD markers |
| title_sort |
molecular phylogenetic relationships among four species of the mangrove tree genus bruguiera (rhizophoraceae), as revealed by chromosome and rapd markers |
| title_alt |
Molecular phylogenetic relationships among four species of the mangrove tree genus Bruguiera (Rhizophoraceae), as revealed by chromosome and RAPD markers |
| publisher |
Universidad de Costa Rica |
| publishDate |
2007 |
| url |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/6023 |
| work_keys_str_mv |
AT sahoopragnya molecularphylogeneticrelationshipsamongfourspeciesofthemangrovetreegenusbruguierarhizophoraceaeasrevealedbychromosomeandrapdmarkers AT jenasatyanarayan molecularphylogeneticrelationshipsamongfourspeciesofthemangrovetreegenusbruguierarhizophoraceaeasrevealedbychromosomeandrapdmarkers AT mohantysuprava molecularphylogeneticrelationshipsamongfourspeciesofthemangrovetreegenusbruguierarhizophoraceaeasrevealedbychromosomeandrapdmarkers AT bandhudasanath molecularphylogeneticrelationshipsamongfourspeciesofthemangrovetreegenusbruguierarhizophoraceaeasrevealedbychromosomeandrapdmarkers |
| _version_ |
1810114582156410880 |