A reliable bioassay procedure to evaluate per os toxicity of Bacillus thuringiensis strains against the rice delphacid, Tagosodes orizicolus (Homoptera: Delphacidae)

Detalles Bibliográficos
Autores principales:	Mora, Rebeca, E. Ibarra, Jorge, M. Espinoza, Ana
Formato:	Online
Idioma:	eng
Publicado:	Universidad de Costa Rica 2007
Acceso en línea:	https://revistas.ucr.ac.cr/index.php/rbt/article/view/6017

id	RBT6017
record_format	ojs
spelling	RBT60172022-05-30T19:05:50Z A reliable bioassay procedure to evaluate per os toxicity of Bacillus thuringiensis strains against the rice delphacid, Tagosodes orizicolus (Homoptera: Delphacidae) A reliable bioassay procedure to evaluate per os toxicity of Bacillus thuringiensis strains against the rice delphacid, Tagosodes orizicolus (Homoptera: Delphacidae) Mora, Rebeca E. Ibarra, Jorge M. Espinoza, Ana Tagosodes orizicolus Homoptera delfácido del arroz bioensayo cría dieta azucarada Bacillus thuringiensis δ-endotoxinas. rice delphacid bioassay rearing sugar diet δ-endotoxins. A reliable bioassay procedure was developed to test ingested Bacillus thuringiensis (Bt) toxins on the rice delphacid Tagosodes orizicolus. Initially, several colonies were established under greenhouse conditions, using rice plants to nurture the insect. For the bioassay, an in vitro feeding system was developed for third to fourth instar nymphs. Insects were fed through Parafilm membranes on sugar (10 % sucrose) and honey bee (1:48 vol/vol) solutions, observing a natural mortality of 10-15 % and 0-5 %, respectively. Results were reproducible under controlled conditions during the assay (18±0.1 °C at night and 28±0.1 °C during the day, 80 % RH and a 12:12 day:light photoperiod). In addition, natural mortality was quantified on insect colonies, collected from three different geographic areas of Costa Rica, with no significant differences between colonies under controlled conditions. Finally, bioassays were performed to evaluate the toxicity of a Bt collection on T. orizicolus. A preliminary sample of twenty-seven Bt strains was evaluated on coarse bioassays using three loops of sporulated colonies in 9 ml of liquid diet, the strains that exhibited higher percentages of T. orizicolus mortality were further analyzed in bioassays using lyophilized spores and crystals (1 mg/ml). As a result, strains 26-O-to, 40-X-m, 43- S-d and 23-O-to isolated from homopteran insects showed mortalities of 74, 96, 44 and 82 % respectively while HD-137, HD-1 and Bti showed 19, 83 and 95 % mortalities. Controls showed mortalities between 0 and 10 % in all bioassays. This is the first report of a reliable bioassay procedure to evaluate per os toxicity for a homopteran species using Bacillus thuringiensis strains. Universidad de Costa Rica 2007-06-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article Text application/pdf https://revistas.ucr.ac.cr/index.php/rbt/article/view/6017 10.15517/rbt.v55i2.6017 Revista de Biología Tropical; Vol. 55 No. 2 (2007): Volume 55 – Regular number 2 – June 2007; 373–383 Revista de Biología Tropical; Vol. 55 Núm. 2 (2007): Volumen 55 – Número regular 2 – Junio 2007; 373–383 Revista Biología Tropical; Vol. 55 N.º 2 (2007): Volume 55 – Regular number 2 – June 2007; 373–383 2215-2075 0034-7744 10.15517/rbt.v55i2 eng https://revistas.ucr.ac.cr/index.php/rbt/article/view/6017/5723 Copyright (c) 2007 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0
institution	Universidad de Costa Rica
collection	Revista de Biología Tropical
language	eng
format	Online
author	Mora, Rebeca E. Ibarra, Jorge M. Espinoza, Ana
spellingShingle	Mora, Rebeca E. Ibarra, Jorge M. Espinoza, Ana A reliable bioassay procedure to evaluate per os toxicity of Bacillus thuringiensis strains against the rice delphacid, Tagosodes orizicolus (Homoptera: Delphacidae)
author_facet	Mora, Rebeca E. Ibarra, Jorge M. Espinoza, Ana
author_sort	Mora, Rebeca
title	A reliable bioassay procedure to evaluate per os toxicity of Bacillus thuringiensis strains against the rice delphacid, Tagosodes orizicolus (Homoptera: Delphacidae)
title_short	A reliable bioassay procedure to evaluate per os toxicity of Bacillus thuringiensis strains against the rice delphacid, Tagosodes orizicolus (Homoptera: Delphacidae)
title_full	A reliable bioassay procedure to evaluate per os toxicity of Bacillus thuringiensis strains against the rice delphacid, Tagosodes orizicolus (Homoptera: Delphacidae)
title_fullStr	A reliable bioassay procedure to evaluate per os toxicity of Bacillus thuringiensis strains against the rice delphacid, Tagosodes orizicolus (Homoptera: Delphacidae)
title_full_unstemmed	A reliable bioassay procedure to evaluate per os toxicity of Bacillus thuringiensis strains against the rice delphacid, Tagosodes orizicolus (Homoptera: Delphacidae)
title_sort	reliable bioassay procedure to evaluate per os toxicity of bacillus thuringiensis strains against the rice delphacid, tagosodes orizicolus (homoptera: delphacidae)
title_alt	A reliable bioassay procedure to evaluate per os toxicity of Bacillus thuringiensis strains against the rice delphacid, Tagosodes orizicolus (Homoptera: Delphacidae)
publisher	Universidad de Costa Rica
publishDate	2007
url	https://revistas.ucr.ac.cr/index.php/rbt/article/view/6017
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A reliable bioassay procedure to evaluate per os toxicity of Bacillus thuringiensis strains against the rice delphacid, Tagosodes orizicolus (Homoptera: Delphacidae)

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