Growth and biosurfactant synthesis by Nigerian hydrocarbon-degrading estuarine bacteria
The ability of microorganisms to degrade petroleum hydrocarbons is important for finding an environmentally-friendly method to restoring contaminated environmental matrices. Screening of hydrocarbonutilizing and biosurfactant-producing abilities of organisms from an estuarine ecosystem in Nigeria, A...
| Autores principales: | , , , , |
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| Formato: | Online |
| Idioma: | eng |
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Universidad de Costa Rica
2008
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| Acceso en línea: | https://revistas.ucr.ac.cr/index.php/rbt/article/view/5746 |
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RBT5746 |
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| record_format |
ojs |
| institution |
Universidad de Costa Rica |
| collection |
Revista de Biología Tropical |
| language |
eng |
| format |
Online |
| author |
Adebusoye, Sunday A. Amund, Olukayode O. Illori, Matthew O. Domeih, Dupe O. Okpuzor, Joy |
| spellingShingle |
Adebusoye, Sunday A. Amund, Olukayode O. Illori, Matthew O. Domeih, Dupe O. Okpuzor, Joy Growth and biosurfactant synthesis by Nigerian hydrocarbon-degrading estuarine bacteria |
| author_facet |
Adebusoye, Sunday A. Amund, Olukayode O. Illori, Matthew O. Domeih, Dupe O. Okpuzor, Joy |
| author_sort |
Adebusoye, Sunday A. |
| description |
The ability of microorganisms to degrade petroleum hydrocarbons is important for finding an environmentally-friendly method to restoring contaminated environmental matrices. Screening of hydrocarbonutilizing and biosurfactant-producing abilities of organisms from an estuarine ecosystem in Nigeria, Africa, resulted in the isolation of five microbial strains identified as Corynebacterium sp. DDV1, Flavobacterium sp. DDV2, Micrococcus roseus DDV3, Pseudomonas aeruginosa DDV4 and Saccharomyces cerevisae DDV5. These isolates grew readily on several hydrocarbons including hexadecane, dodecane, crude oil and petroleum fractions. Axenic cultures of the organisms utilized diesel oil (1.0 % v/v) with generation times that ranged significantly (t-test, P < 0.05) between 3.25 and 3.88 day, with concomitant production of biosurfactants. Kinetics of growth indicates that biosurfactant synthesis occurred predominantly during exponential growth phase, suggesting that the bioactive molecules are primary metabolites. Strains DDV1 and DDV4 were evidently the most metabolically active in terms of substrate utilization and biosurfactant synthesis compared to other strains with respective emulsification index of 63 and 78 %. Preliminary biochemical characterization indicates that the biosurfactants are heteropolymers consisting of lipid, protein and carbohydrate moieties. The hydrocarbon catabolic properties coupled with biosurfactant-producing capabilities is an asset that could be exploited for cleanup of oil-contaminated matrices and also in food and cosmetic industries. |
| title |
Growth and biosurfactant synthesis by Nigerian hydrocarbon-degrading estuarine bacteria |
| title_short |
Growth and biosurfactant synthesis by Nigerian hydrocarbon-degrading estuarine bacteria |
| title_full |
Growth and biosurfactant synthesis by Nigerian hydrocarbon-degrading estuarine bacteria |
| title_fullStr |
Growth and biosurfactant synthesis by Nigerian hydrocarbon-degrading estuarine bacteria |
| title_full_unstemmed |
Growth and biosurfactant synthesis by Nigerian hydrocarbon-degrading estuarine bacteria |
| title_sort |
growth and biosurfactant synthesis by nigerian hydrocarbon-degrading estuarine bacteria |
| title_alt |
Growth and biosurfactant synthesis by Nigerian hydrocarbon-degrading estuarine bacteria |
| publisher |
Universidad de Costa Rica |
| publishDate |
2008 |
| url |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/5746 |
| work_keys_str_mv |
AT adebusoyesundaya growthandbiosurfactantsynthesisbynigerianhydrocarbondegradingestuarinebacteria AT amundolukayodeo growthandbiosurfactantsynthesisbynigerianhydrocarbondegradingestuarinebacteria AT illorimatthewo growthandbiosurfactantsynthesisbynigerianhydrocarbondegradingestuarinebacteria AT domeihdupeo growthandbiosurfactantsynthesisbynigerianhydrocarbondegradingestuarinebacteria AT okpuzorjoy growthandbiosurfactantsynthesisbynigerianhydrocarbondegradingestuarinebacteria |
| _version_ |
1810114569277800448 |
| spelling |
RBT57462022-06-06T18:05:27Z Growth and biosurfactant synthesis by Nigerian hydrocarbon-degrading estuarine bacteria Growth and biosurfactant synthesis by Nigerian hydrocarbon-degrading estuarine bacteria Adebusoye, Sunday A. Amund, Olukayode O. Illori, Matthew O. Domeih, Dupe O. Okpuzor, Joy Bio-surfactante diesel hidrocarburos biodegradación cepas bacterianas Biosurfactant diesel oil hydrocarbon biodegradation bacterial strains The ability of microorganisms to degrade petroleum hydrocarbons is important for finding an environmentally-friendly method to restoring contaminated environmental matrices. Screening of hydrocarbonutilizing and biosurfactant-producing abilities of organisms from an estuarine ecosystem in Nigeria, Africa, resulted in the isolation of five microbial strains identified as Corynebacterium sp. DDV1, Flavobacterium sp. DDV2, Micrococcus roseus DDV3, Pseudomonas aeruginosa DDV4 and Saccharomyces cerevisae DDV5. These isolates grew readily on several hydrocarbons including hexadecane, dodecane, crude oil and petroleum fractions. Axenic cultures of the organisms utilized diesel oil (1.0 % v/v) with generation times that ranged significantly (t-test, P < 0.05) between 3.25 and 3.88 day, with concomitant production of biosurfactants. Kinetics of growth indicates that biosurfactant synthesis occurred predominantly during exponential growth phase, suggesting that the bioactive molecules are primary metabolites. Strains DDV1 and DDV4 were evidently the most metabolically active in terms of substrate utilization and biosurfactant synthesis compared to other strains with respective emulsification index of 63 and 78 %. Preliminary biochemical characterization indicates that the biosurfactants are heteropolymers consisting of lipid, protein and carbohydrate moieties. The hydrocarbon catabolic properties coupled with biosurfactant-producing capabilities is an asset that could be exploited for cleanup of oil-contaminated matrices and also in food and cosmetic industries. The ability of microorganisms to degrade petroleum hydrocarbons is important for finding an environmentally-friendly method to restoring contaminated environmental matrices. Screening of hydrocarbonutilizing and biosurfactant-producing abilities of organisms from an estuarine ecosystem in Nigeria, Africa, resulted in the isolation of five microbial strains identified as Corynebacterium sp. DDV1, Flavobacterium sp. DDV2, Micrococcus roseus DDV3, Pseudomonas aeruginosa DDV4 and Saccharomyces cerevisae DDV5. These isolates grew readily on several hydrocarbons including hexadecane, dodecane, crude oil and petroleum fractions. Axenic cultures of the organisms utilized diesel oil (1.0 % v/v) with generation times that ranged significantly (t-test, P < 0.05) between 3.25 and 3.88 day, with concomitant production of biosurfactants. Kinetics of growth indicates that biosurfactant synthesis occurred predominantly during exponential growth phase, suggesting that the bioactive molecules are primary metabolites. Strains DDV1 and DDV4 were evidently the most metabolically active in terms of substrate utilization and biosurfactant synthesis compared to other strains with respective emulsification index of 63 and 78 %. Preliminary biochemical characterization indicates that the biosurfactants are heteropolymers consisting of lipid, protein and carbohydrate moieties. The hydrocarbon catabolic properties coupled with biosurfactant-producing capabilities is an asset that could be exploited for cleanup of oil-contaminated matrices and also in food and cosmetic industries. Universidad de Costa Rica 2008-12-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article Text application/pdf https://revistas.ucr.ac.cr/index.php/rbt/article/view/5746 10.15517/rbt.v56i4.5746 Revista de Biología Tropical; Vol. 56 No. 4 (2008): Volume 56 – Regular number 4 – December 2008; 1603–1611 Revista de Biología Tropical; Vol. 56 Núm. 4 (2008): Volumen 56 – Número regular 4 – Diciembre 2008; 1603–1611 Revista Biología Tropical; Vol. 56 N.º 4 (2008): Volume 56 – Regular number 4 – December 2008; 1603–1611 2215-2075 0034-7744 10.15517/rbt.v56i4 eng https://revistas.ucr.ac.cr/index.php/rbt/article/view/5746/5487 Copyright (c) 2008 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0 |