Humoral response of paracoccidioidomycosis sera in hamsters with different Venezuelan isolates
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| Formato: | Online |
| Idioma: | eng |
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Universidad de Costa Rica
2009
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| Acceso en línea: | https://revistas.ucr.ac.cr/index.php/rbt/article/view/5471 |
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RBT54712022-06-06T18:14:30Z Humoral response of paracoccidioidomycosis sera in hamsters with different Venezuelan isolates Humoral response of paracoccidioidomycosis sera in hamsters with different Venezuelan isolates M. Spencer, Lilian Mateu, Guaniri Magaldi, Sylvia García, Fabiola Mata-Essayag, Sofia respuesta humoral Paracoccidioides brasiliensis paracoccidioidomicosis hámsteres Syrian aislados venezolanos ELISA Immunoblotting humoral response paracoccidioidomycosis Syrian hamsters Venezuelan isolates Humoral response of paracoccidioidomycosis sera in hamsters with different Venezuelan isolates. Paracoccidioidomycosis (PCM) is a progressive systemic mycosis caused by the fungus Paraccocidioides brasiliensis (Pb), endemic to Venezuela and Latin America. In this study, eight different Venezuelan isolates obtained from patients with PCM, were inoculated intraperitoneally in Syrian hamsters (Cricetus auratus) and studied by immune-serum. Each strain was collected by gently scraping the surface of the culture medium (Sabouraud Dextrose Agar) and suspended in 3ml of 0.15 M phosphate-buffered saline. The antigen obtained was called Paraccocidioides brasiliensis Crude Antigen (CAP). Immunoblotting results showed that the immune-sera from hamsters recognized at least 3 bands: one over 200 kDa, and two of 80 and 15-20 kDa. This study suggests that IgG anti-CAP can reveal a significant variability in the eight Venezuelan isolates. Sera from 88 infected hamsters were evaluated by ELISA with eight different CAPs and Western blot with CAP 37383. ELISA results showed that, the antigen of the virulent isolate 37383 had the highest percentage (38%) of positivity, while the nonvirulent isolate 1458 had the lowest one (13.6%). Furthermore, scanning densitometry revealed that the isolate 37383 had less bands than the non-virulent isolates. These results suggest that the ELISA test with CAP 37383 can detect circulating antibodies, and that this virulent isolate may be useful for the diagnosis of PCM, and to monitor disease responses to treatments. Universidad de Costa Rica 2009-09-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article Text application/pdf https://revistas.ucr.ac.cr/index.php/rbt/article/view/5471 10.15517/rbt.v57i3.5471 Revista de Biología Tropical; Vol. 57 No. 3 (2009): Volume 57 – Regular number 3 – September 2009; 505−513 Revista de Biología Tropical; Vol. 57 Núm. 3 (2009): Volumen 57 – Número regular 3 – Setiembre 2009; 505−513 Revista Biología Tropical; Vol. 57 N.º 3 (2009): Volume 57 – Regular number 3 – September 2009; 505−513 2215-2075 0034-7744 10.15517/rbt.v57i3 eng https://revistas.ucr.ac.cr/index.php/rbt/article/view/5471/5217 Copyright (c) 2009 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0 |
| institution |
Universidad de Costa Rica |
| collection |
Revista de Biología Tropical |
| language |
eng |
| format |
Online |
| author |
M. Spencer, Lilian Mateu, Guaniri Magaldi, Sylvia García, Fabiola Mata-Essayag, Sofia |
| spellingShingle |
M. Spencer, Lilian Mateu, Guaniri Magaldi, Sylvia García, Fabiola Mata-Essayag, Sofia Humoral response of paracoccidioidomycosis sera in hamsters with different Venezuelan isolates |
| author_facet |
M. Spencer, Lilian Mateu, Guaniri Magaldi, Sylvia García, Fabiola Mata-Essayag, Sofia |
| author_sort |
M. Spencer, Lilian |
| title |
Humoral response of paracoccidioidomycosis sera in hamsters with different Venezuelan isolates |
| title_short |
Humoral response of paracoccidioidomycosis sera in hamsters with different Venezuelan isolates |
| title_full |
Humoral response of paracoccidioidomycosis sera in hamsters with different Venezuelan isolates |
| title_fullStr |
Humoral response of paracoccidioidomycosis sera in hamsters with different Venezuelan isolates |
| title_full_unstemmed |
Humoral response of paracoccidioidomycosis sera in hamsters with different Venezuelan isolates |
| title_sort |
humoral response of paracoccidioidomycosis sera in hamsters with different venezuelan isolates |
| title_alt |
Humoral response of paracoccidioidomycosis sera in hamsters with different Venezuelan isolates |
| publisher |
Universidad de Costa Rica |
| publishDate |
2009 |
| url |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/5471 |
| work_keys_str_mv |
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