Variabilidad genética del pez Lutjanus guttatus (Perciformes: Lutjanidae) mediante el uso de microsatélites en Costa Rica

Variabilidad genética del pez Lutjanus guttatus (Perciformes: Lutjanidae) mediante el uso de microsatélites en Costa Rica

Introduction: The rose spotted snapper (Lutjanus guttatus) is a highly consumed marine fish and of commercial interest in Costa Rica, under a strong fishing pressure that can affect the genetic diversity of the population creating problems related to inbreeding depression. Objective:  To ev...

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Autor principal: Murillo Rios, Alejandra
Formato: Online
Idioma:spa
Publicado: Universidad de Costa Rica 2023
Acceso en línea:https://revistas.ucr.ac.cr/index.php/rbt/article/view/53380
id RBT53380
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spelling RBT533802024-10-29T21:43:35Z Genetic variability of the fish Lutjanus guttatus (Perciformes: Lutjanidae) using microsatellites in Costa Rica Variabilidad genética del pez Lutjanus guttatus (Perciformes: Lutjanidae) mediante el uso de microsatélites en Costa Rica Murillo Rios, Alejandra rose spotted snapper heterozygosity inbreeding stock enhancement Introduction: The rose spotted snapper (Lutjanus guttatus) is a highly consumed marine fish and of commercial interest in Costa Rica, under a strong fishing pressure that can affect the genetic diversity of the population creating problems related to inbreeding depression. Objective:  To evaluate the genetic diversity of the spotted snapper (Lutjanus guttatus) population in Costa Rica using microsatellites. Methodology: Samples were collected from two locations (Golfo Dulce and Golfo de Nicoya) in 2019, and 44 individuals from each site were studied. DNA extraction and amplification of 10 microsatellites by PCR were carried out for subsequent genotyping for genetic diversity and population structure analysis. Results: The genetic diversity parameters show a high polymorphism, associated to a high number of alleles per locus, but low levels of observed heterozygosity in comparison to the expected heterozygosity (Ho = 0,774 and 0,800 and He = 0,948 and 0,954 for Gulf of Nicoya and Gulf Dulce, respectively). The study of population differentiation indicates that there is not enough evidence to ensure the two populations are different (FST = 0,00264, p > 0.05). The significant deviation from Hardy-Weinberg Equilibrium obtained indicated the possible presence of organisms in the population that does not come from the wild. Conclusions: The rose spotted snapper in Costa Rica shows high levels of genetic diversity, without evidence of differentiation in genetic subpopulations, that should be considered as a single panmictic population for fishery management purposes. The possible mix of individuals of a distinct origin found in the wild suggests the presence of organisms that come from a stock enhancement program or from commercial aquaculture carried out in the region. The use of genetic markers is recommended to keep track of the genetic characteristics of the wild population, to follow-up stock enhancement programs, and eventually, asses their effect. Universidad de Costa Rica 2023-07-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html application/epub+zip https://revistas.ucr.ac.cr/index.php/rbt/article/view/53380 10.15517/rev.biol.trop..v71i1.53380 Revista de Biología Tropical; Vol. 71 No. 1 (2023): Revista de Biología Tropical (Rev. Biol. Trop.): Continuous publication, 01 January - 31 December 2023; e53380 Revista de Biología Tropical; Vol. 71 Núm. 1 (2023): Revista de Biología Tropical (Rev. Biol. Trop.): Continuous publication, 01 January - 31 December 2023; e53380 Revista Biología Tropical; Vol. 71 N.º 1 (2023): Revista de Biología Tropical (Rev. Biol. Trop.): Continuous publication, 01 January - 31 December 2023; e53380 2215-2075 0034-7744 10.15517/rev.biol.trop..v71i1.2023 spa https://revistas.ucr.ac.cr/index.php/rbt/article/view/53380/56635 https://revistas.ucr.ac.cr/index.php/rbt/article/view/53380/56636 https://revistas.ucr.ac.cr/index.php/rbt/article/view/53380/56637 Copyright (c) 2023 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0
institution Universidad de Costa Rica
collection Revista de Biología Tropical
language spa
format Online
author Murillo Rios, Alejandra
spellingShingle Murillo Rios, Alejandra
Variabilidad genética del pez Lutjanus guttatus (Perciformes: Lutjanidae) mediante el uso de microsatélites en Costa Rica
author_facet Murillo Rios, Alejandra
author_sort Murillo Rios, Alejandra
description Introduction: The rose spotted snapper (Lutjanus guttatus) is a highly consumed marine fish and of commercial interest in Costa Rica, under a strong fishing pressure that can affect the genetic diversity of the population creating problems related to inbreeding depression. Objective:  To evaluate the genetic diversity of the spotted snapper (Lutjanus guttatus) population in Costa Rica using microsatellites. Methodology: Samples were collected from two locations (Golfo Dulce and Golfo de Nicoya) in 2019, and 44 individuals from each site were studied. DNA extraction and amplification of 10 microsatellites by PCR were carried out for subsequent genotyping for genetic diversity and population structure analysis. Results: The genetic diversity parameters show a high polymorphism, associated to a high number of alleles per locus, but low levels of observed heterozygosity in comparison to the expected heterozygosity (Ho = 0,774 and 0,800 and He = 0,948 and 0,954 for Gulf of Nicoya and Gulf Dulce, respectively). The study of population differentiation indicates that there is not enough evidence to ensure the two populations are different (FST = 0,00264, p > 0.05). The significant deviation from Hardy-Weinberg Equilibrium obtained indicated the possible presence of organisms in the population that does not come from the wild. Conclusions: The rose spotted snapper in Costa Rica shows high levels of genetic diversity, without evidence of differentiation in genetic subpopulations, that should be considered as a single panmictic population for fishery management purposes. The possible mix of individuals of a distinct origin found in the wild suggests the presence of organisms that come from a stock enhancement program or from commercial aquaculture carried out in the region. The use of genetic markers is recommended to keep track of the genetic characteristics of the wild population, to follow-up stock enhancement programs, and eventually, asses their effect.
title Variabilidad genética del pez Lutjanus guttatus (Perciformes: Lutjanidae) mediante el uso de microsatélites en Costa Rica
title_short Variabilidad genética del pez Lutjanus guttatus (Perciformes: Lutjanidae) mediante el uso de microsatélites en Costa Rica
title_full Variabilidad genética del pez Lutjanus guttatus (Perciformes: Lutjanidae) mediante el uso de microsatélites en Costa Rica
title_fullStr Variabilidad genética del pez Lutjanus guttatus (Perciformes: Lutjanidae) mediante el uso de microsatélites en Costa Rica
title_full_unstemmed Variabilidad genética del pez Lutjanus guttatus (Perciformes: Lutjanidae) mediante el uso de microsatélites en Costa Rica
title_sort variabilidad genética del pez lutjanus guttatus (perciformes: lutjanidae) mediante el uso de microsatélites en costa rica
title_alt Genetic variability of the fish Lutjanus guttatus (Perciformes: Lutjanidae) using microsatellites in Costa Rica
publisher Universidad de Costa Rica
publishDate 2023
url https://revistas.ucr.ac.cr/index.php/rbt/article/view/53380
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