Pastas de Rhodomonas salina (Cryptophyta) como alimento para Brachionus plicatilis (Rotifera)

Rhodomonas salina (Cryptophyta) pastes as feed for Brachionus plicatilis (Rotifera). Rotifers are an important live feed for first feeding larvae of many fish species. The use of concentrated algae cells in the mass culture of the rotifer Brachionus plicatilis (Brachionidae) has opened new horizons...

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Autores principales:	Guevara, Miguel, Bastardo, Leandro, Cortez, Roraysi, Arredondo-Vega, Bertha, Romero, Lolymar, Gómez, Patricia
Formato:	Online
Idioma:	spa
Publicado:	Universidad de Costa Rica 2011
Acceso en línea:	https://revistas.ucr.ac.cr/index.php/rbt/article/view/3415

id	RBT3415
record_format	ojs
spelling	RBT34152022-06-06T18:59:48Z Pastas de Rhodomonas salina (Cryptophyta) como alimento para Brachionus plicatilis (Rotifera) Guevara, Miguel Bastardo, Leandro Cortez, Roraysi Arredondo-Vega, Bertha Romero, Lolymar Gómez, Patricia microalgae paste aquaculture Rhodomonas salina flocculation rotifers pasta de microalgas acuicultura floculación rotíferos Rhodomonas salina (Cryptophyta) pastes as feed for Brachionus plicatilis (Rotifera). Rotifers are an important live feed for first feeding larvae of many fish species. The use of concentrated algae cells in the mass culture of the rotifer Brachionus plicatilis (Brachionidae) has opened new horizons for research on this organism. Pastes of Rhodomonas salina (Pyrenomonadaceae) obtained either by centrifugation or flocculation with chitosan were preserved, with or without vitamin C, at -20°C for four weeks and were evaluated biochemically (proteins, lipids, pigments and fatty acids contents) and subsequently, were used to feed the rotifer Brachionus plicatilis at a ratio of 25mg/L/day. Four different microalgae pastes were prepared: (1) centrifuged and preserved with vitamin C (CV), (2) centrifuged and preserved without vitamin C (C), (3) flocculated and with vitamin C (FV) and (4) flocculated without vitamin C (F). All treatments showed similar contents of proteins and total lipids with respect to control culture (a fresh culture of R. salina), with mean values of 40.0±2.32% and 12.0±1.45%, respectively. The pheophytin a/chlorophyll a ratio, a general indicator of the chemical status of microalgal concentrates, was similar (0.09-0.11) between centrifuged pastes and control culture, but was found to be higher in flocculated pastes (1.28-1.48). The fatty acid profile varied with respect to the control culture, mainly in the proportion of the essential polyunsaturated fatty acids (PUFAs): eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Total PUFAs, EPA and DHA contents were statistically similar between centrifuged pastes and control culture (PUFAs: 47%, EPA: 4% and DHA: 4.7%), whereas values obtained for flocculated pastes were significantly lower. The rotifers grew equally well when fed with centrifuged pastes or control culture (maximum density: 320rotifers/mL; instantaneous growth rate: 0.23rotifers/ day, fecundity: 1.49eggs/female and productivity: 43x103rotifers/L/day. No significant effect of vitamin C was found when used as a paste preservative. We concluded that centrifugation is an effective harvesting method, and that freezing to -20°C for four weeks (no vitamin added), may help maintain the nutritional quality of R. salina paste, similar to fresh microalgae and can be offered to Brachionus plicatilis. Universidad de Costa Rica 2011-12-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article Text application/pdf https://revistas.ucr.ac.cr/index.php/rbt/article/view/3415 10.15517/rbt.v59i4.3415 Revista de Biología Tropical; Vol. 59 No. 4 (2011): Volume 59 – Regular number 4 – December 2011; 1503–1515 Revista de Biología Tropical; Vol. 59 Núm. 4 (2011): Volumen 59 – Número regular 4 – Diciembre 2011; 1503–1515 Revista Biología Tropical; Vol. 59 N.º 4 (2011): Volume 59 – Regular number 4 – December 2011; 1503–1515 2215-2075 0034-7744 10.15517/rbt.v59i4 spa https://revistas.ucr.ac.cr/index.php/rbt/article/view/3415/3319 Copyright (c) 2011 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0
institution	Universidad de Costa Rica
collection	Revista de Biología Tropical
language	spa
format	Online
author	Guevara, Miguel Bastardo, Leandro Cortez, Roraysi Arredondo-Vega, Bertha Romero, Lolymar Gómez, Patricia
spellingShingle	Guevara, Miguel Bastardo, Leandro Cortez, Roraysi Arredondo-Vega, Bertha Romero, Lolymar Gómez, Patricia Pastas de Rhodomonas salina (Cryptophyta) como alimento para Brachionus plicatilis (Rotifera)
author_facet	Guevara, Miguel Bastardo, Leandro Cortez, Roraysi Arredondo-Vega, Bertha Romero, Lolymar Gómez, Patricia
author_sort	Guevara, Miguel
description	Rhodomonas salina (Cryptophyta) pastes as feed for Brachionus plicatilis (Rotifera). Rotifers are an important live feed for first feeding larvae of many fish species. The use of concentrated algae cells in the mass culture of the rotifer Brachionus plicatilis (Brachionidae) has opened new horizons for research on this organism. Pastes of Rhodomonas salina (Pyrenomonadaceae) obtained either by centrifugation or flocculation with chitosan were preserved, with or without vitamin C, at -20°C for four weeks and were evaluated biochemically (proteins, lipids, pigments and fatty acids contents) and subsequently, were used to feed the rotifer Brachionus plicatilis at a ratio of 25mg/L/day. Four different microalgae pastes were prepared: (1) centrifuged and preserved with vitamin C (CV), (2) centrifuged and preserved without vitamin C (C), (3) flocculated and with vitamin C (FV) and (4) flocculated without vitamin C (F). All treatments showed similar contents of proteins and total lipids with respect to control culture (a fresh culture of R. salina), with mean values of 40.0±2.32% and 12.0±1.45%, respectively. The pheophytin a/chlorophyll a ratio, a general indicator of the chemical status of microalgal concentrates, was similar (0.09-0.11) between centrifuged pastes and control culture, but was found to be higher in flocculated pastes (1.28-1.48). The fatty acid profile varied with respect to the control culture, mainly in the proportion of the essential polyunsaturated fatty acids (PUFAs): eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Total PUFAs, EPA and DHA contents were statistically similar between centrifuged pastes and control culture (PUFAs: 47%, EPA: 4% and DHA: 4.7%), whereas values obtained for flocculated pastes were significantly lower. The rotifers grew equally well when fed with centrifuged pastes or control culture (maximum density: 320rotifers/mL; instantaneous growth rate: 0.23rotifers/ day, fecundity: 1.49eggs/female and productivity: 43x103rotifers/L/day. No significant effect of vitamin C was found when used as a paste preservative. We concluded that centrifugation is an effective harvesting method, and that freezing to -20°C for four weeks (no vitamin added), may help maintain the nutritional quality of R. salina paste, similar to fresh microalgae and can be offered to Brachionus plicatilis.
title	Pastas de Rhodomonas salina (Cryptophyta) como alimento para Brachionus plicatilis (Rotifera)
title_short	Pastas de Rhodomonas salina (Cryptophyta) como alimento para Brachionus plicatilis (Rotifera)
title_full	Pastas de Rhodomonas salina (Cryptophyta) como alimento para Brachionus plicatilis (Rotifera)
title_fullStr	Pastas de Rhodomonas salina (Cryptophyta) como alimento para Brachionus plicatilis (Rotifera)
title_full_unstemmed	Pastas de Rhodomonas salina (Cryptophyta) como alimento para Brachionus plicatilis (Rotifera)
title_sort	pastas de rhodomonas salina (cryptophyta) como alimento para brachionus plicatilis (rotifera)
publisher	Universidad de Costa Rica
publishDate	2011
url	https://revistas.ucr.ac.cr/index.php/rbt/article/view/3415
work_keys_str_mv	AT guevaramiguel pastasderhodomonassalinacryptophytacomoalimentoparabrachionusplicatilisrotifera AT bastardoleandro pastasderhodomonassalinacryptophytacomoalimentoparabrachionusplicatilisrotifera AT cortezroraysi pastasderhodomonassalinacryptophytacomoalimentoparabrachionusplicatilisrotifera AT arredondovegabertha pastasderhodomonassalinacryptophytacomoalimentoparabrachionusplicatilisrotifera AT romerololymar pastasderhodomonassalinacryptophytacomoalimentoparabrachionusplicatilisrotifera AT gomezpatricia pastasderhodomonassalinacryptophytacomoalimentoparabrachionusplicatilisrotifera
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Pastas de Rhodomonas salina (Cryptophyta) como alimento para Brachionus plicatilis (Rotifera)

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