Caracterización molecular y resistencia antimicrobiana de aislamientos de Clostridium perfringens de diferentes orígenes en Costa Rica

Molecular characterization and antimicrobial resistance of Clostridium perfringens isolates of different origins from Costa Rica. Clostridium perfringens, a Gram positive, spore-forming anaerobe, is widely distributed in nature. Based upon their production of four major toxins α, β, ε and ι, C. perf...

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Autores principales:	del Mar Gamboa-Coronado, María, Mau-Inchaustegui, Silvia, Rodríguez-Cavallini, Evelyn
Formato:	Online
Idioma:	spa
Publicado:	Universidad de Costa Rica 2011
Acceso en línea:	https://revistas.ucr.ac.cr/index.php/rbt/article/view/3413

id	RBT3413
record_format	ojs
spelling	RBT34132022-06-06T18:59:48Z Caracterización molecular y resistencia antimicrobiana de aislamientos de Clostridium perfringens de diferentes orígenes en Costa Rica del Mar Gamboa-Coronado, María Mau-Inchaustegui, Silvia Rodríguez-Cavallini, Evelyn Clostridium perfringens enterotoxin toxinotypes antimicrobial resistance enterotoxinas toxinotipos resistencia antimicrobiana Molecular characterization and antimicrobial resistance of Clostridium perfringens isolates of different origins from Costa Rica. Clostridium perfringens, a Gram positive, spore-forming anaerobe, is widely distributed in nature. Based upon their production of four major toxins α, β, ε and ι, C. perfringens is classified into five toxinotypes (A-E). Some strains produce an enterotoxin (CPE), encoded by the cpe gene, which causes diarrhea in humans and some animals. C. perfringens strains that had been previously isolated and been kept at -80°C were analyzed for the presence of toxin genes and for antimicrobial resistance: 20 from soils, 20 from animal, 20 from human origin and 21 from food non related to outbreaks. According to PCR results, all strains were classified as C. perfringens type A, since only a toxin gene was detected, while cpe was detected in two strains (2.5%) isolated from food, as it has been described in other world regions. Antibiotic resistance to at least one antibiotic was detected in 44% of the strains, 41% was resistant to clindamycin, 25% to chloramphenicol, 22% to penicillin and 20% to metronidazole. Soils strains showed the highest resistance percentages to almost all antibiotics. Multiresistance (to three or more antibiotic groups) was detected in the strains from soil (40%), human origin (30%), food (14%) and animal origin (5%). The high resistance rates found may be explained by the widespread use of antimicrobials as growth promoters in plants and animals; also these resistant strains may act as reservoir of resistance genes that may be transferred between bacteria in different environments. Universidad de Costa Rica 2011-12-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article Text application/pdf https://revistas.ucr.ac.cr/index.php/rbt/article/view/3413 10.15517/rbt.v59i4.3413 Revista de Biología Tropical; Vol. 59 No. 4 (2011): Volume 59 – Regular number 4 – December 2011; 1479–1485 Revista de Biología Tropical; Vol. 59 Núm. 4 (2011): Volumen 59 – Número regular 4 – Diciembre 2011; 1479–1485 Revista Biología Tropical; Vol. 59 N.º 4 (2011): Volume 59 – Regular number 4 – December 2011; 1479–1485 2215-2075 0034-7744 10.15517/rbt.v59i4 spa https://revistas.ucr.ac.cr/index.php/rbt/article/view/3413/3317 Copyright (c) 2011 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0
institution	Universidad de Costa Rica
collection	Revista de Biología Tropical
language	spa
format	Online
author	del Mar Gamboa-Coronado, María Mau-Inchaustegui, Silvia Rodríguez-Cavallini, Evelyn
spellingShingle	del Mar Gamboa-Coronado, María Mau-Inchaustegui, Silvia Rodríguez-Cavallini, Evelyn Caracterización molecular y resistencia antimicrobiana de aislamientos de Clostridium perfringens de diferentes orígenes en Costa Rica
author_facet	del Mar Gamboa-Coronado, María Mau-Inchaustegui, Silvia Rodríguez-Cavallini, Evelyn
author_sort	del Mar Gamboa-Coronado, María
description	Molecular characterization and antimicrobial resistance of Clostridium perfringens isolates of different origins from Costa Rica. Clostridium perfringens, a Gram positive, spore-forming anaerobe, is widely distributed in nature. Based upon their production of four major toxins α, β, ε and ι, C. perfringens is classified into five toxinotypes (A-E). Some strains produce an enterotoxin (CPE), encoded by the cpe gene, which causes diarrhea in humans and some animals. C. perfringens strains that had been previously isolated and been kept at -80°C were analyzed for the presence of toxin genes and for antimicrobial resistance: 20 from soils, 20 from animal, 20 from human origin and 21 from food non related to outbreaks. According to PCR results, all strains were classified as C. perfringens type A, since only a toxin gene was detected, while cpe was detected in two strains (2.5%) isolated from food, as it has been described in other world regions. Antibiotic resistance to at least one antibiotic was detected in 44% of the strains, 41% was resistant to clindamycin, 25% to chloramphenicol, 22% to penicillin and 20% to metronidazole. Soils strains showed the highest resistance percentages to almost all antibiotics. Multiresistance (to three or more antibiotic groups) was detected in the strains from soil (40%), human origin (30%), food (14%) and animal origin (5%). The high resistance rates found may be explained by the widespread use of antimicrobials as growth promoters in plants and animals; also these resistant strains may act as reservoir of resistance genes that may be transferred between bacteria in different environments.
title	Caracterización molecular y resistencia antimicrobiana de aislamientos de Clostridium perfringens de diferentes orígenes en Costa Rica
title_short	Caracterización molecular y resistencia antimicrobiana de aislamientos de Clostridium perfringens de diferentes orígenes en Costa Rica
title_full	Caracterización molecular y resistencia antimicrobiana de aislamientos de Clostridium perfringens de diferentes orígenes en Costa Rica
title_fullStr	Caracterización molecular y resistencia antimicrobiana de aislamientos de Clostridium perfringens de diferentes orígenes en Costa Rica
title_full_unstemmed	Caracterización molecular y resistencia antimicrobiana de aislamientos de Clostridium perfringens de diferentes orígenes en Costa Rica
title_sort	caracterización molecular y resistencia antimicrobiana de aislamientos de clostridium perfringens de diferentes orígenes en costa rica
publisher	Universidad de Costa Rica
publishDate	2011
url	https://revistas.ucr.ac.cr/index.php/rbt/article/view/3413
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Caracterización molecular y resistencia antimicrobiana de aislamientos de Clostridium perfringens de diferentes orígenes en Costa Rica

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