Caracterización fenotípica y genotípica de doce rizobios aislados de diversas regiones geográficas de Venezuela
Rhizobial taxonomy and systematics have progressed substantially, nevertheless, few studies have been developed on venezuelan species. This study evaluated the phenotypic and genetic variation between 12 venezuelan indigenous rhizobial isolates and 10 international referential strains, by phenotypic...
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| Formato: | Online |
| Idioma: | spa |
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Universidad de Costa Rica
2011
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| Acceso en línea: | https://revistas.ucr.ac.cr/index.php/rbt/article/view/3375 |
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RBT3375 |
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| record_format |
ojs |
| institution |
Universidad de Costa Rica |
| collection |
Revista de Biología Tropical |
| language |
spa |
| format |
Online |
| author |
Eugenia Marquina, María Enrique González, Néstor Castro, Yulimar |
| spellingShingle |
Eugenia Marquina, María Enrique González, Néstor Castro, Yulimar Caracterización fenotípica y genotípica de doce rizobios aislados de diversas regiones geográficas de Venezuela |
| author_facet |
Eugenia Marquina, María Enrique González, Néstor Castro, Yulimar |
| author_sort |
Eugenia Marquina, María |
| description |
Rhizobial taxonomy and systematics have progressed substantially, nevertheless, few studies have been developed on venezuelan species. This study evaluated the phenotypic and genetic variation between 12 venezuelan indigenous rhizobial isolates and 10 international referential strains, by phenotypical traits and DNA molecular markers. In this regard, a PCR-RFLP of the 16S rDNA gene, the presence of large plasmids, metabolic assays in solid media, salinity resistance, pH and temperature growth conditions, and intrinsic antibiotic resistance were assayed. In reference to the phenotypic attributes, we recognized three main groups: A group I, which comprised all the strains metabolizing between 67.5%-90% of the C and N sources. They were also acid-tolerant, as well as acid producers, capable of growing at 40°C and in high salinity conditions (2-2.5% NaCl). With regard to the antibiotic sensitivity, this group was susceptible to a 30% of the antibiotic assayed. Strains belonging to Group II exhibited a lower salt tolerance (0.1-1.5%NaCl), as well as a lower acid tolerance, since they grew well at pH values equal or higher than 5.0. This group appeared to be resistant to all of the antibiotics assayed and only metabolized between 52.5%-82.5% of the C and N sources. Group III was represented by a single bacterial strain: it has a extremely low salt tolerance (0.1% NaCl). This strain grew at a pH equal or higher than 5.6, was susceptible to 50% of the antibiotics assayed and metabolized 72% of the C and N sources. On the basis of a PCR- RFLP of the 16S rDNA, three groups were also obtained. Members of the group A showed a close resemblance to Rhizobium tropici CIAT 899 and Sinorhizobium americanum CFN-EI 156, while Group B was closely related to Bradyrhizobium spp. Group C, was also represented by only one isolate. The Trebol isolate, was the only one strain able to form nodules and does not appear to be related to any of the referential rhizobial strains, suggesting a possible symbiotic horizontal gene transfer. Finally, in this work, there are evidences of a genetic diversity in the venezuelan rhizobial strains. A different geographical origin is perhaps an important factor affecting the diversity of the indigenous rhizobia in this study. |
| title |
Caracterización fenotípica y genotípica de doce rizobios aislados de diversas regiones geográficas de Venezuela |
| title_short |
Caracterización fenotípica y genotípica de doce rizobios aislados de diversas regiones geográficas de Venezuela |
| title_full |
Caracterización fenotípica y genotípica de doce rizobios aislados de diversas regiones geográficas de Venezuela |
| title_fullStr |
Caracterización fenotípica y genotípica de doce rizobios aislados de diversas regiones geográficas de Venezuela |
| title_full_unstemmed |
Caracterización fenotípica y genotípica de doce rizobios aislados de diversas regiones geográficas de Venezuela |
| title_sort |
caracterización fenotípica y genotípica de doce rizobios aislados de diversas regiones geográficas de venezuela |
| title_alt |
Caracterización fenotípica y genotípica de doce rizobios aislados de diversas regiones geográficas de Venezuela |
| publisher |
Universidad de Costa Rica |
| publishDate |
2011 |
| url |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/3375 |
| work_keys_str_mv |
AT eugeniamarquinamaria caracterizacionfenotipicaygenotipicadedocerizobiosaisladosdediversasregionesgeograficasdevenezuela AT enriquegonzaleznestor caracterizacionfenotipicaygenotipicadedocerizobiosaisladosdediversasregionesgeograficasdevenezuela AT castroyulimar caracterizacionfenotipicaygenotipicadedocerizobiosaisladosdediversasregionesgeograficasdevenezuela |
| _version_ |
1810114509105266688 |
| spelling |
RBT33752022-06-06T18:58:21Z Caracterización fenotípica y genotípica de doce rizobios aislados de diversas regiones geográficas de Venezuela Caracterización fenotípica y genotípica de doce rizobios aislados de diversas regiones geográficas de Venezuela Eugenia Marquina, María Enrique González, Néstor Castro, Yulimar Rhizobium Bradyrhizobium phenotypic characterization PCR-RFLP 16S rDNA caracterización fenotípica ADNr 16S Rhizobial taxonomy and systematics have progressed substantially, nevertheless, few studies have been developed on venezuelan species. This study evaluated the phenotypic and genetic variation between 12 venezuelan indigenous rhizobial isolates and 10 international referential strains, by phenotypical traits and DNA molecular markers. in this regard, a PCR-RFLP of the 16S rDNA gene, the presence of large plasmids, metabolic assays in solid media, salinity resistance, pH and temperature growth conditions, and intrinsic antibiotic resis-tance were assayed. in reference to the phenotypic attributes, we recognized three main groups: A group i, which comprised all the strains metabolizing between 67.5%-90% of the C and N sources. They were also acid-tolerant, as well as acid producers, capable of growing at 40ºC and in high salinity conditions (2-2.5% NaCl). With regard to the antibiotic sensitivity, this group was susceptible to a 30% of the antibiotic assayed. Strains belonging to Group ii exhibited a lower salt tolerance (0.1-1.5%NaCl), as well as a lower acid tolerance, since they grew well at pH values equal or higher than 5.0. This group appeared to be resistant to all of the antibiotics assayed and only metabolized between 52.5%-82.5% of the C and N sources. Group iii was represented by a single bacte-rial strain: it has a extremely low salt tolerance (0.1% NaCl). This strain grew at a pH equal or higher than 5.6, was susceptible to 50% of the antibiotics assayed and metabolized 72% of the C and N sources. On the basis of a PCR- RFLP of the 16S rDNA, three groups were also obtained. Members of the group A showed a close resemblance to Rhizobium tropici CiAT 899 and Sinorhizobium americanum CFN-Ei 156, while Group B was closely related to Bradyrhizobium spp. Group C, was also represented by only one isolate. The Trebol isolate, was the only one strain able to form nodules and does not appear to be related to any of the referential rhizobial strains, suggesting a possible symbiotic horizontal gene transfer. Finally, in this work, there are evidences of a genetic diversity in the venezuelan rhizobial strains. A different geographical origin is perhaps an important factor affecting the diversity of the indigenous rhizobia in this study. Rhizobial taxonomy and systematics have progressed substantially, nevertheless, few studies have been developed on venezuelan species. This study evaluated the phenotypic and genetic variation between 12 venezuelan indigenous rhizobial isolates and 10 international referential strains, by phenotypical traits and DNA molecular markers. In this regard, a PCR-RFLP of the 16S rDNA gene, the presence of large plasmids, metabolic assays in solid media, salinity resistance, pH and temperature growth conditions, and intrinsic antibiotic resistance were assayed. In reference to the phenotypic attributes, we recognized three main groups: A group I, which comprised all the strains metabolizing between 67.5%-90% of the C and N sources. They were also acid-tolerant, as well as acid producers, capable of growing at 40°C and in high salinity conditions (2-2.5% NaCl). With regard to the antibiotic sensitivity, this group was susceptible to a 30% of the antibiotic assayed. Strains belonging to Group II exhibited a lower salt tolerance (0.1-1.5%NaCl), as well as a lower acid tolerance, since they grew well at pH values equal or higher than 5.0. This group appeared to be resistant to all of the antibiotics assayed and only metabolized between 52.5%-82.5% of the C and N sources. Group III was represented by a single bacterial strain: it has a extremely low salt tolerance (0.1% NaCl). This strain grew at a pH equal or higher than 5.6, was susceptible to 50% of the antibiotics assayed and metabolized 72% of the C and N sources. On the basis of a PCR- RFLP of the 16S rDNA, three groups were also obtained. Members of the group A showed a close resemblance to Rhizobium tropici CIAT 899 and Sinorhizobium americanum CFN-EI 156, while Group B was closely related to Bradyrhizobium spp. Group C, was also represented by only one isolate. The Trebol isolate, was the only one strain able to form nodules and does not appear to be related to any of the referential rhizobial strains, suggesting a possible symbiotic horizontal gene transfer. Finally, in this work, there are evidences of a genetic diversity in the venezuelan rhizobial strains. A different geographical origin is perhaps an important factor affecting the diversity of the indigenous rhizobia in this study. Universidad de Costa Rica 2011-09-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article Text application/pdf https://revistas.ucr.ac.cr/index.php/rbt/article/view/3375 10.15517/rbt.v0i0.3375 Revista de Biología Tropical; Vol. 59 No. 3 (2011): Volume 59 – Regular number 3 – September 2011; 1017–1036 Revista de Biología Tropical; Vol. 59 Núm. 3 (2011): Volumen 59 – Número regular 3 – Setiembre 2011; 1017–1036 Revista Biología Tropical; Vol. 59 N.º 3 (2011): Volume 59 – Regular number 3 – September 2011; 1017–1036 2215-2075 0034-7744 10.15517/rbt.v0i0 spa https://revistas.ucr.ac.cr/index.php/rbt/article/view/3375/3279 Copyright (c) 2011 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0 |