Micropropagation of Baccharis antioquensis (Asteraceae) and photoinduction of polyphenols by UV radiation

Detalles Bibliográficos
Autores principales: Naranjo-Gómez, Esther J., Puertas-Mejía, Miguel A., Mejía-Giraldo, Juan C., Amaya-Nieto, Anggy Z., Atehortúa, Lucia
Formato: Online
Idioma:eng
Publicado: Universidad de Costa Rica 2018
Acceso en línea:https://revistas.ucr.ac.cr/index.php/rbt/article/view/33406
id RBT33406
record_format ojs
spelling RBT334062022-06-09T18:14:13Z Micropropagation of Baccharis antioquensis (Asteraceae) and photoinduction of polyphenols by UV radiation Naranjo-Gómez, Esther J. Puertas-Mejía, Miguel A. Mejía-Giraldo, Juan C. Amaya-Nieto, Anggy Z. Atehortúa, Lucia B. antioquensis elicitation growth regulators UVR in vitro plants. Polyphenols are a large diversity of chemical types and interactions that can be responsible for a multiplicity of protective functions ranging from toxicity and light/UV shielding to signal transduction. Bacharis antioquensis has been described as a potential source of new photoprotective compounds with antioxidant capacity associated to polyphenols compounds. The aim of the present work was to develop a micropropagation protocol of B. antioquensis and evaluate the production of polyphenols by in vitro plants exposure to UVB radiation. Branches in juvenile stage of B. antioquensis were collected, desinfected and cultured on half strength Murashige and Skoog medium, supplemented or not with growth regulators (TDZ, BA or GA3) on light/darkness conditions and liquid/solid media. After UV treatments, the absorption coefficient in the UVA-UVB range, the antioxidant capacity and the total phenol content (TPC) from all tissue cultures and the wild tissue were evaluated. Growth regulators, light conditions and type of culture medium (solid or liquid) had a favorable effect on the response of explants. Treatments containing BA + GA3 regulators (2 and 0.5 mg/L respectively) and TDZ (0.5 mg/L) showed positive results in bud growth in liquid medium and darkness. Results showed that UVR exhibited promoting effects on the accumulation of polyphenols, enhancing the absorption coefficient in the UVA-UVB range, the antiradical capacity and the TPC of B. antioquensis in vitro plants. Rev. Biol. Trop. 66(2): 754-764. Epub 2018 June 01.   Universidad de Costa Rica 2018-06-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article application/pdf text/html https://revistas.ucr.ac.cr/index.php/rbt/article/view/33406 10.15517/rbt.v66i2.33406 Revista de Biología Tropical; Vol. 66 No. 2 (2018): Volume 66 – Regular number 2 – June 2018; 754–764 Revista de Biología Tropical; Vol. 66 Núm. 2 (2018): Volumen 66 – Número regular 2 – Junio 2018; 754–764 Revista Biología Tropical; Vol. 66 N.º 2 (2018): Volume 66 – Regular number 2 – June 2018; 754–764 2215-2075 0034-7744 10.15517/rbt.v66i2 eng https://revistas.ucr.ac.cr/index.php/rbt/article/view/33406/32878 https://revistas.ucr.ac.cr/index.php/rbt/article/view/33406/32915 Copyright (c) 2018 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0
institution Universidad de Costa Rica
collection Revista de Biología Tropical
language eng
format Online
author Naranjo-Gómez, Esther J.
Puertas-Mejía, Miguel A.
Mejía-Giraldo, Juan C.
Amaya-Nieto, Anggy Z.
Atehortúa, Lucia
spellingShingle Naranjo-Gómez, Esther J.
Puertas-Mejía, Miguel A.
Mejía-Giraldo, Juan C.
Amaya-Nieto, Anggy Z.
Atehortúa, Lucia
Micropropagation of Baccharis antioquensis (Asteraceae) and photoinduction of polyphenols by UV radiation
author_facet Naranjo-Gómez, Esther J.
Puertas-Mejía, Miguel A.
Mejía-Giraldo, Juan C.
Amaya-Nieto, Anggy Z.
Atehortúa, Lucia
author_sort Naranjo-Gómez, Esther J.
title Micropropagation of Baccharis antioquensis (Asteraceae) and photoinduction of polyphenols by UV radiation
title_short Micropropagation of Baccharis antioquensis (Asteraceae) and photoinduction of polyphenols by UV radiation
title_full Micropropagation of Baccharis antioquensis (Asteraceae) and photoinduction of polyphenols by UV radiation
title_fullStr Micropropagation of Baccharis antioquensis (Asteraceae) and photoinduction of polyphenols by UV radiation
title_full_unstemmed Micropropagation of Baccharis antioquensis (Asteraceae) and photoinduction of polyphenols by UV radiation
title_sort micropropagation of baccharis antioquensis (asteraceae) and photoinduction of polyphenols by uv radiation
publisher Universidad de Costa Rica
publishDate 2018
url https://revistas.ucr.ac.cr/index.php/rbt/article/view/33406
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