Cytogenetic bioassays as tools to distinguish between toxic and non-toxic varieties of Jatropha curcas (Euphorbiaceae)
| Autores principales: | , , , , , |
|---|---|
| Formato: | Online |
| Idioma: | eng |
| Publicado: |
Universidad de Costa Rica
2018
|
| Acceso en línea: | https://revistas.ucr.ac.cr/index.php/rbt/article/view/33377 |
| id |
RBT33377 |
|---|---|
| record_format |
ojs |
| spelling |
RBT333772022-06-09T18:14:13Z Cytogenetic bioassays as tools to distinguish between toxic and non-toxic varieties of Jatropha curcas (Euphorbiaceae) Fonseca Andrade-Vieira, Larissa Mastella Botelho, Carolina Pinheiro, Carlos Alexandre Fontes Pinheiro, Patrícia Galvêas Laviola, Bruno Miranda Praça-Fontes, Milene root growth seed germination phytotoxicity phorbol ester mitodepressive effect. The tropical and subtropical naturalized physic nut (Jatropha curcas L.), has been explored for biodiesel production in recent times. The oil is extracted from the seeds and, for the production to be feasible, utilization of the residual seed cake is crucial. Although the cake could be employed as a protein source in animal feed, it is rich in phorbol ester, which is toxic for animals. Therefore, breeding programs have been working to reduce or eliminate the phorbol ester content in physic nut. In this context, the present work aimed to evaluate the physic nut oil of toxic and non-toxic varieties (containing known or undetectable amounts of phorbol ester, respectively) with regards to phytotoxicity in a model experiment with Lactuca sativa L. For this, the percentage of germinated seeds was evaluated after 8, 16, 24, 36 and 48 hours of exposure to the treatments with toxic and non-toxic oil at concentrations of 22.5 %, 45 % and 67.5 % of emulsion (physic nut oil energetically mixed with distilled water). Root growth was determined after 48 hours of exposure and the germination speed index was obtained. The different stages of mitotic division as well as possible chromosomal and nuclear alterations were also recorded. The mitotic index was calculated as the number of dividing cells, as a fraction of the total number of cells, and the frequency of chromosome and nuclear alterations, expressed as the percentage of number of alterations divided by the total number of cells. Both varieties exhibited phytotoxicity, inducing significant reductions in percentage of germinated seeds (reduction of 98 %), germination speed index (reduction of 24.44) and root growth (reduction of 8.54 mm). In microscopic analysis, a mitodepressive effect was observed for both oils at the three concentrations used when compared to the negative control; however, it was possible to distinguish between the toxic and the non-toxic varieties based on the more expressive reduction of division promoted by the first, 2.19 %. Significant increments in the frequency of mitotic cells showing chromosome alterations as well, as the presence of condensed nuclei, were observed in the treated cells. However, these parameters were not significantly different from the control in the cells treated with both physic nut oils. In conclusion, the evaluation of root growth and cell division in the plant model L. sativa, can be proposed as an alternative to animal tests to distinguish the varieties with high and low phorbol ester concentration, thus contributing to the detection of toxicity in varieties used in breeding programs. Rev. Biol. Trop. 66(2): 495-502. Epub 2018 June 01. Universidad de Costa Rica 2018-06-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article application/pdf text/html text/html https://revistas.ucr.ac.cr/index.php/rbt/article/view/33377 10.15517/rbt.v66i2.33377 Revista de Biología Tropical; Vol. 66 No. 2 (2018): Volume 66 – Regular number 2 – June 2018; 495–502 Revista de Biología Tropical; Vol. 66 Núm. 2 (2018): Volumen 66 – Número regular 2 – Junio 2018; 495–502 Revista Biología Tropical; Vol. 66 N.º 2 (2018): Volume 66 – Regular number 2 – June 2018; 495–502 2215-2075 0034-7744 10.15517/rbt.v66i2 eng https://revistas.ucr.ac.cr/index.php/rbt/article/view/33377/32859 https://revistas.ucr.ac.cr/index.php/rbt/article/view/33377/32897 https://revistas.ucr.ac.cr/index.php/rbt/article/view/33377/32898 Copyright (c) 2018 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0 |
| institution |
Universidad de Costa Rica |
| collection |
Revista de Biología Tropical |
| language |
eng |
| format |
Online |
| author |
Fonseca Andrade-Vieira, Larissa Mastella Botelho, Carolina Pinheiro, Carlos Alexandre Fontes Pinheiro, Patrícia Galvêas Laviola, Bruno Miranda Praça-Fontes, Milene |
| spellingShingle |
Fonseca Andrade-Vieira, Larissa Mastella Botelho, Carolina Pinheiro, Carlos Alexandre Fontes Pinheiro, Patrícia Galvêas Laviola, Bruno Miranda Praça-Fontes, Milene Cytogenetic bioassays as tools to distinguish between toxic and non-toxic varieties of Jatropha curcas (Euphorbiaceae) |
| author_facet |
Fonseca Andrade-Vieira, Larissa Mastella Botelho, Carolina Pinheiro, Carlos Alexandre Fontes Pinheiro, Patrícia Galvêas Laviola, Bruno Miranda Praça-Fontes, Milene |
| author_sort |
Fonseca Andrade-Vieira, Larissa |
| title |
Cytogenetic bioassays as tools to distinguish between toxic and non-toxic varieties of Jatropha curcas (Euphorbiaceae) |
| title_short |
Cytogenetic bioassays as tools to distinguish between toxic and non-toxic varieties of Jatropha curcas (Euphorbiaceae) |
| title_full |
Cytogenetic bioassays as tools to distinguish between toxic and non-toxic varieties of Jatropha curcas (Euphorbiaceae) |
| title_fullStr |
Cytogenetic bioassays as tools to distinguish between toxic and non-toxic varieties of Jatropha curcas (Euphorbiaceae) |
| title_full_unstemmed |
Cytogenetic bioassays as tools to distinguish between toxic and non-toxic varieties of Jatropha curcas (Euphorbiaceae) |
| title_sort |
cytogenetic bioassays as tools to distinguish between toxic and non-toxic varieties of jatropha curcas (euphorbiaceae) |
| publisher |
Universidad de Costa Rica |
| publishDate |
2018 |
| url |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/33377 |
| work_keys_str_mv |
AT fonsecaandradevieiralarissa cytogeneticbioassaysastoolstodistinguishbetweentoxicandnontoxicvarietiesofjatrophacurcaseuphorbiaceae AT mastellabotelhocarolina cytogeneticbioassaysastoolstodistinguishbetweentoxicandnontoxicvarietiesofjatrophacurcaseuphorbiaceae AT pinheirocarlosalexandre cytogeneticbioassaysastoolstodistinguishbetweentoxicandnontoxicvarietiesofjatrophacurcaseuphorbiaceae AT fontespinheiropatricia cytogeneticbioassaysastoolstodistinguishbetweentoxicandnontoxicvarietiesofjatrophacurcaseuphorbiaceae AT galveaslaviolabruno cytogeneticbioassaysastoolstodistinguishbetweentoxicandnontoxicvarietiesofjatrophacurcaseuphorbiaceae AT mirandapracafontesmilene cytogeneticbioassaysastoolstodistinguishbetweentoxicandnontoxicvarietiesofjatrophacurcaseuphorbiaceae |
| _version_ |
1810115227256094720 |