In vitro rhizogenesis: histoanatomy of Cedrela odorata (Meliaceae) microcuttings
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| Formato: | Online |
| Idioma: | eng |
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Universidad de Costa Rica
2011
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| Acceso en línea: | https://revistas.ucr.ac.cr/index.php/rbt/article/view/3211 |
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RBT3211 |
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RBT32112022-06-06T18:54:02Z In vitro rhizogenesis: histoanatomy of Cedrela odorata (Meliaceae) microcuttings In vitro rhizogenesis: histoanatomy of Cedrela odorata (Meliaceae) microcuttings Millán-Orozco, Liliana Corredoira, Elena del Carmen San José, Maria cultivo in vitro enraizaimiento adventicio estudio anatómico cedro histología in vitro culture adventitious rooting anatomical study Spanish cedar histology Cedrela odorata (Meliaceae) is considered as one of the most valuable forest tree in the tropics. Clonal propagation of this species provide an alternative method to propagate superior genotypes, being the production of good quality adventitious roots one of the most important steps in micropropagation techniques. The sequence of anatomical changes that takes place during the formation of adventitious roots in shoots of Cedrela odorata cultured in vitro is described in this study. Eigth-week-old shoots, from multiplication cultures, were rooted in Murashige and Skoog's medium (1962) with half-strength macronutrients and with 0 or 1mg/l indole-3-butyric acid (IBA). Between 12 and 24h after the start of rooting, some cambium, phloem and interfascicular parenchyma cells became dense cytoplasm, nuclei with prominent nucleoli and the first cell divisions were observed, especially in shoots treated with auxin (dedifferentiation phase). After 3-4 days, the number of dedifferentiated cells and mitotic divisions increased considerably, and the formation of groups of some 30-40 meristematic cells (meristemoids) was observed (induction phase). The first primordial roots developed from the 4th-5th day. The vascular tissues of these primordia connected to those of the explant, and roots began to emerge from the base by day 6. Development of the primordial roots was similar in the control shoots and shoots treated with 1mg/l IBA, although there were more roots per explant in the latter. Rev. Biol. Trop. 59 (1): 447-453. Epub 2011 March 01. Universidad de Costa Rica 2011-03-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article Text application/pdf https://revistas.ucr.ac.cr/index.php/rbt/article/view/3211 10.15517/rbt.v59i1.3211 Revista de Biología Tropical; Vol. 59 No. 1 (2011): Volume 59 – Regular number 1 – March 2011; 447–453 Revista de Biología Tropical; Vol. 59 Núm. 1 (2011): Volumen 59 – Número regular 1 – Marzo 2011; 447–453 Revista Biología Tropical; Vol. 59 N.º 1 (2011): Volume 59 – Regular number 1 – March 2011; 447–453 2215-2075 0034-7744 10.15517/rbt.v59i1 eng https://revistas.ucr.ac.cr/index.php/rbt/article/view/3211/3117 Copyright (c) 2011 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0 |
| institution |
Universidad de Costa Rica |
| collection |
Revista de Biología Tropical |
| language |
eng |
| format |
Online |
| author |
Millán-Orozco, Liliana Corredoira, Elena del Carmen San José, Maria |
| spellingShingle |
Millán-Orozco, Liliana Corredoira, Elena del Carmen San José, Maria In vitro rhizogenesis: histoanatomy of Cedrela odorata (Meliaceae) microcuttings |
| author_facet |
Millán-Orozco, Liliana Corredoira, Elena del Carmen San José, Maria |
| author_sort |
Millán-Orozco, Liliana |
| title |
In vitro rhizogenesis: histoanatomy of Cedrela odorata (Meliaceae) microcuttings |
| title_short |
In vitro rhizogenesis: histoanatomy of Cedrela odorata (Meliaceae) microcuttings |
| title_full |
In vitro rhizogenesis: histoanatomy of Cedrela odorata (Meliaceae) microcuttings |
| title_fullStr |
In vitro rhizogenesis: histoanatomy of Cedrela odorata (Meliaceae) microcuttings |
| title_full_unstemmed |
In vitro rhizogenesis: histoanatomy of Cedrela odorata (Meliaceae) microcuttings |
| title_sort |
in vitro rhizogenesis: histoanatomy of cedrela odorata (meliaceae) microcuttings |
| title_alt |
In vitro rhizogenesis: histoanatomy of Cedrela odorata (Meliaceae) microcuttings |
| publisher |
Universidad de Costa Rica |
| publishDate |
2011 |
| url |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/3211 |
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