Factors influencing rapid clonal propagation of Chlorophytum arundinaceum (Liliales: Liliaceae), an endangered medicinal plant
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| Formato: | Online |
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Universidad de Costa Rica
2011
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| Acceso en línea: | https://revistas.ucr.ac.cr/index.php/rbt/article/view/3210 |
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RBT32102022-06-06T18:54:02Z Factors influencing rapid clonal propagation of Chlorophytum arundinaceum (Liliales: Liliaceae), an endangered medicinal plant Factors influencing rapid clonal propagation of Chlorophytum arundinaceum (Liliales: Liliaceae), an endangered medicinal plant Samantaray, Sanghamitra Maiti, Satyabrata ddd Liliaceae medicinal plant pH sucrose Chlorophytum arundinaceum is an important medicinal plant and its tuberous roots are used for various health ailment treatments. It has become an endangered species in the Eastern Ghats, and a rare medicinal herb in India, due to its excessive collection from its natural habitat and its destructive harvesting techniques, coupled with poor seed germination and low vegetative multiplication ratio. In order to contribute to its production systems, an efficient protocol was developed for in vitro clonal propagation through shoot bud culture. For this, multiple shoots were induced from shoot bud explants on Murashige and Skoog's medium supplemented with 2.5-3.0mg/L BAP, 0.01-0.1mg/L NAA and 3% (w/v) sucrose. Inclusion of Adenine Sulphate (25mg/L) in the culture medium improved the frequency of multiple shoot production and recovered the chlorotic symptoms of the leaves. Media having pH 5.9 and 4% sucrose showed significant improvement on shoot bud multiplication and growth. In vitro flowering was observed when the subcultures were carried out for over four months in the same multiplication media. Rooting was readily achieved upon transferring the shoots on to half- strength MS medium supplemented with 0.1mg/L IBA and 2% (w/v) sucrose. Micropropagated plantlets were hardened in the green house, successfully established, and flowered in the field. This method could effectively be applied for the conservation and clonal propagation to meet the demand of planting materials. Rev. Biol. Trop. 59 (1): 435-445. Epub 2011 March 01. Universidad de Costa Rica 2011-03-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article Text application/pdf https://revistas.ucr.ac.cr/index.php/rbt/article/view/3210 10.15517/rbt.v59i1.3210 Revista de Biología Tropical; Vol. 59 No. 1 (2011): Volume 59 – Regular number 1 – March 2011; 435–445 Revista de Biología Tropical; Vol. 59 Núm. 1 (2011): Volumen 59 – Número regular 1 – Marzo 2011; 435–445 Revista Biología Tropical; Vol. 59 N.º 1 (2011): Volume 59 – Regular number 1 – March 2011; 435–445 2215-2075 0034-7744 10.15517/rbt.v59i1 eng https://revistas.ucr.ac.cr/index.php/rbt/article/view/3210/3116 Copyright (c) 2011 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0 |
| institution |
Universidad de Costa Rica |
| collection |
Revista de Biología Tropical |
| language |
eng |
| format |
Online |
| author |
Samantaray, Sanghamitra Maiti, Satyabrata |
| spellingShingle |
Samantaray, Sanghamitra Maiti, Satyabrata Factors influencing rapid clonal propagation of Chlorophytum arundinaceum (Liliales: Liliaceae), an endangered medicinal plant |
| author_facet |
Samantaray, Sanghamitra Maiti, Satyabrata |
| author_sort |
Samantaray, Sanghamitra |
| title |
Factors influencing rapid clonal propagation of Chlorophytum arundinaceum (Liliales: Liliaceae), an endangered medicinal plant |
| title_short |
Factors influencing rapid clonal propagation of Chlorophytum arundinaceum (Liliales: Liliaceae), an endangered medicinal plant |
| title_full |
Factors influencing rapid clonal propagation of Chlorophytum arundinaceum (Liliales: Liliaceae), an endangered medicinal plant |
| title_fullStr |
Factors influencing rapid clonal propagation of Chlorophytum arundinaceum (Liliales: Liliaceae), an endangered medicinal plant |
| title_full_unstemmed |
Factors influencing rapid clonal propagation of Chlorophytum arundinaceum (Liliales: Liliaceae), an endangered medicinal plant |
| title_sort |
factors influencing rapid clonal propagation of chlorophytum arundinaceum (liliales: liliaceae), an endangered medicinal plant |
| title_alt |
Factors influencing rapid clonal propagation of Chlorophytum arundinaceum (Liliales: Liliaceae), an endangered medicinal plant |
| publisher |
Universidad de Costa Rica |
| publishDate |
2011 |
| url |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/3210 |
| work_keys_str_mv |
AT samantaraysanghamitra factorsinfluencingrapidclonalpropagationofchlorophytumarundinaceumlilialesliliaceaeanendangeredmedicinalplant AT maitisatyabrata factorsinfluencingrapidclonalpropagationofchlorophytumarundinaceumlilialesliliaceaeanendangeredmedicinalplant |
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1810114507670814720 |