Estructura y la ultraestructura del ovario de Cichlasoma urophthalmus (Perciformes: Cichlidae)
The study of the normal development, differentiation, structure and function of various components of developing follicles in the ovaries of numerous fish species have been a consistent focus of comparative reproduction. The structural and ultrastructural features of gonads from Cichlasoma urophthal...
| Autores principales: | , , , , , |
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| Formato: | Online |
| Idioma: | spa |
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Universidad de Costa Rica
2011
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| Acceso en línea: | https://revistas.ucr.ac.cr/index.php/rbt/article/view/3136 |
| id |
RBT3136 |
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| record_format |
ojs |
| institution |
Universidad de Costa Rica |
| collection |
Revista de Biología Tropical |
| language |
spa |
| format |
Online |
| author |
Viedma, Rubí Franco, Jonathan Bedia, Carlos Guedea Fernández, Guadalupe Barrera Villa Zevallos, Héctor Barrera Escorcia, Héctor |
| spellingShingle |
Viedma, Rubí Franco, Jonathan Bedia, Carlos Guedea Fernández, Guadalupe Barrera Villa Zevallos, Héctor Barrera Escorcia, Héctor Estructura y la ultraestructura del ovario de Cichlasoma urophthalmus (Perciformes: Cichlidae) |
| author_facet |
Viedma, Rubí Franco, Jonathan Bedia, Carlos Guedea Fernández, Guadalupe Barrera Villa Zevallos, Héctor Barrera Escorcia, Héctor |
| author_sort |
Viedma, Rubí |
| description |
The study of the normal development, differentiation, structure and function of various components of developing follicles in the ovaries of numerous fish species have been a consistent focus of comparative reproduction. The structural and ultrastructural features of gonads from Cichlasoma urophthalmus have received scarce attention. In this work, we realized a descriptive study of female gonads of Cichlasoma urophthalmus. A total of 40 samples were collected in the Veracruz Alvarado Lagoon, Mexico in 2007-2008 period including the windy, dry and rainy seasons. Female gonads were extracted and a portion was fixed in 4% formaldehyde for treatment for routine histology hematoxylin and eosin (HE) and another part was processed for transmission electron microscopy (TEM). The gonads were fixed in 3% glutaraldehyde and 2% osmium tetroxide, followed by dehydrated in ethanol 50%, 70%, 80%, 95% and 100% for inclusion in Epon, thin sections were then prepared and were contrasted with lead citrate and uranyl acetate. The process of oocyte development can be divided into five distinct stages (formation of oocytes from oogonia, primary growth, lipid stage, vitellogenesis and maturation). In this work, we found that the primary growth stage is characterized by intense RNA synthesis and the differentiation of the vitelline envelope. Secondary growth starts with the accumulation of lipid droplets in the oocyte cytoplasm (lipid stage), which is then followed by massive uptake and processing of proteins into yolk platelets (vitellogenic stage). During the maturation stage, the lipid inclusions coalesce into a single oil droplet, and hydrolysis of the yolk platelets leads to the formation of a homogeneous mass of fluid yolk in mature eggs. In conclusion, further studies should elucidate structure and ultrastructural changes in the ovarian follicular components, in C. urophthalmus during different stages of oocyte growth. |
| title |
Estructura y la ultraestructura del ovario de Cichlasoma urophthalmus (Perciformes: Cichlidae) |
| title_short |
Estructura y la ultraestructura del ovario de Cichlasoma urophthalmus (Perciformes: Cichlidae) |
| title_full |
Estructura y la ultraestructura del ovario de Cichlasoma urophthalmus (Perciformes: Cichlidae) |
| title_fullStr |
Estructura y la ultraestructura del ovario de Cichlasoma urophthalmus (Perciformes: Cichlidae) |
| title_full_unstemmed |
Estructura y la ultraestructura del ovario de Cichlasoma urophthalmus (Perciformes: Cichlidae) |
| title_sort |
estructura y la ultraestructura del ovario de cichlasoma urophthalmus (perciformes: cichlidae) |
| title_alt |
Estructura y la ultraestructura del ovario de Cichlasoma urophthalmus (Perciformes: Cichlidae) |
| publisher |
Universidad de Costa Rica |
| publishDate |
2011 |
| url |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/3136 |
| work_keys_str_mv |
AT viedmarubi estructuraylaultraestructuradelovariodecichlasomaurophthalmusperciformescichlidae AT francojonathan estructuraylaultraestructuradelovariodecichlasomaurophthalmusperciformescichlidae AT bediacarlos estructuraylaultraestructuradelovariodecichlasomaurophthalmusperciformescichlidae AT guedeafernandezguadalupe estructuraylaultraestructuradelovariodecichlasomaurophthalmusperciformescichlidae AT barreravillazevalloshector estructuraylaultraestructuradelovariodecichlasomaurophthalmusperciformescichlidae AT barreraescorciahector estructuraylaultraestructuradelovariodecichlasomaurophthalmusperciformescichlidae |
| _version_ |
1810114500272062464 |
| spelling |
RBT31362022-06-06T18:56:43Z Estructura y la ultraestructura del ovario de Cichlasoma urophthalmus (Perciformes: Cichlidae) Estructura y la ultraestructura del ovario de Cichlasoma urophthalmus (Perciformes: Cichlidae) Viedma, Rubí Franco, Jonathan Bedia, Carlos Guedea Fernández, Guadalupe Barrera Villa Zevallos, Héctor Barrera Escorcia, Héctor C. urophthalmus ovaries estructure and ultraestructure oocyte ovarios estructura y ultraestructura The study of the normal development, differentiation, structure and function of various components of develop-ing follicles in the ovaries of numerous fish species have been a consistent focus of comparative reproduction. The structural and ultrastructural features of gonads from Cichlasoma urophthalmus have received scarce atten-tion. In this work, we realized a descriptive study of female gonads of Cichlasoma urophthalmus. A total of 40 samples were collected in the Veracruz Alvarado Lagoon, Mexico in 2007-2008 period including the windy, dry and rainyseasons. Female gonads were extracted and a portion was fixed in 4% formaldehyde for treatment for routine histology hematoxylin and eosin (HE) and another part was processed for transmission electron microscopy (TEM). The gonads were fixed in 3% glutaraldehyde and 2% osmium tetroxide, followed by dehy-drated in ethanol 50%, 70%, 80%, 95% and 100% for inclusion in Epon, thin sections were then prepared and were contrasted with lead citrate and uranyl acetate. The process of oocyte development can be divided into five distinct stages (formation of oocytes from oogonia, primary growth, lipid stage, vitellogenesis and matura-tion). In this work, we found that the primary growth stage is characterized by intense RNA synthesis and the differentiation of the vitelline envelope. Secondary growth starts with the accumulation of lipid droplets in the oocyte cytoplasm (lipid stage), which is then followed by massive uptake and processing of proteins into yolk platelets (vitellogenic stage). During the maturation stage, the lipid inclusions coalesce into a single oil droplet, and hydrolysis of the yolk platelets leads to the formation of a homogeneous mass of fluid yolk in mature eggs. In conclusion, further studies should elucidate structure and ultrastructural changes in the ovarian follicular components, in C. urophthalmus during different stages of oocyte growth. The study of the normal development, differentiation, structure and function of various components of developing follicles in the ovaries of numerous fish species have been a consistent focus of comparative reproduction. The structural and ultrastructural features of gonads from Cichlasoma urophthalmus have received scarce attention. In this work, we realized a descriptive study of female gonads of Cichlasoma urophthalmus. A total of 40 samples were collected in the Veracruz Alvarado Lagoon, Mexico in 2007-2008 period including the windy, dry and rainy seasons. Female gonads were extracted and a portion was fixed in 4% formaldehyde for treatment for routine histology hematoxylin and eosin (HE) and another part was processed for transmission electron microscopy (TEM). The gonads were fixed in 3% glutaraldehyde and 2% osmium tetroxide, followed by dehydrated in ethanol 50%, 70%, 80%, 95% and 100% for inclusion in Epon, thin sections were then prepared and were contrasted with lead citrate and uranyl acetate. The process of oocyte development can be divided into five distinct stages (formation of oocytes from oogonia, primary growth, lipid stage, vitellogenesis and maturation). In this work, we found that the primary growth stage is characterized by intense RNA synthesis and the differentiation of the vitelline envelope. Secondary growth starts with the accumulation of lipid droplets in the oocyte cytoplasm (lipid stage), which is then followed by massive uptake and processing of proteins into yolk platelets (vitellogenic stage). During the maturation stage, the lipid inclusions coalesce into a single oil droplet, and hydrolysis of the yolk platelets leads to the formation of a homogeneous mass of fluid yolk in mature eggs. In conclusion, further studies should elucidate structure and ultrastructural changes in the ovarian follicular components, in C. urophthalmus during different stages of oocyte growth. Universidad de Costa Rica 2011-06-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article Text application/pdf https://revistas.ucr.ac.cr/index.php/rbt/article/view/3136 10.15517/rbt.v0i0.3136 Revista de Biología Tropical; Vol. 59 No. 2 (2011): Volume 59 – Regular number 2 – June 2011; 743–750 Revista de Biología Tropical; Vol. 59 Núm. 2 (2011): Volumen 59 – Número regular 2 – Junio 2011; 743–750 Revista Biología Tropical; Vol. 59 N.º 2 (2011): Volume 59 – Regular number 2 – June 2011; 743–750 2215-2075 0034-7744 10.15517/rbt.v0i0 spa https://revistas.ucr.ac.cr/index.php/rbt/article/view/3136/3043 Copyright (c) 2011 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0 |