A rotary shadow freeze-drying replica method for the three dimensional view of virus ultrastructure

A rotary shadow freeze-drying replica method for the three dimensional view of virus ultrastructure

Se describe un método de sombreado rotatorio para preparar réplicas (copias) de viriones estabilizados mediante congelación y secado a baja presión. Esta réplicas permiten el análisis de la ultraestructura viral; empleándose como modelo de estudio la cápside interna de rotavirus humanos. Los hallazg...

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Detalles Bibliográficos
Autores principales: Hernández, Francisco, Akahori, Hiroshi, Hosaka, Yasuhiro, Akaike, Kaoru, Ikegami, Nobuko
Formato: Online
Idioma:eng
Publicado: Universidad de Costa Rica 1994
Acceso en línea:https://revistas.ucr.ac.cr/index.php/rbt/article/view/28716
id RBT28716
record_format ojs
spelling RBT287162023-08-30T20:53:42Z A rotary shadow freeze-drying replica method for the three dimensional view of virus ultrastructure A rotary shadow freeze-drying replica method for the three dimensional view of virus ultrastructure Hernández, Francisco Akahori, Hiroshi Hosaka, Yasuhiro Akaike, Kaoru Ikegami, Nobuko Rotavirus freeze-drying replica technique ultrastructure A new rotary-shadowing process to obtain freeze-drying replicas is described for the analysis of virus ultrastructure, using the inner capsid of human rotavirus as a model. The findings corroborate the icosahedral symmetry with an arrangement pattern of capsomers of T=13L. Se describe un método de sombreado rotatorio para preparar réplicas (copias) de viriones estabilizados mediante congelación y secado a baja presión. Esta réplicas permiten el análisis de la ultraestructura viral; empleándose como modelo de estudio la cápside interna de rotavirus humanos. Los hallazgos corroboran el patrón de simetría icosaédrico con un patrón de capsómeros de T=13L, previamente descrito. Universidad de Costa Rica 1994-08-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article application/pdf https://revistas.ucr.ac.cr/index.php/rbt/article/view/28716 Revista de Biología Tropical; Vol. 42 No. S2 (1994): Volume 42 – Supplement 2 – August 1994: An electron microscope view of tropical nature: selected studies; 175–178 Revista de Biología Tropical; Vol. 42 Núm. S2 (1994): Volumen 42 – Suplemento 2 – Agosto 1994: La naturaleza tropical vista al microscopio electrónico: estudios selectos; 175–178 Revista Biología Tropical; Vol. 42 N.º S2 (1994): Volume 42 – Supplement 2 – August 1994: An electron microscope view of tropical nature: selected studies; 175–178 2215-2075 0034-7744 10.15517/rbt.v42i2 eng https://revistas.ucr.ac.cr/index.php/rbt/article/view/28716/28696 Copyright (c) 1994 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0
institution Universidad de Costa Rica
collection Revista de Biología Tropical
language eng
format Online
author Hernández, Francisco
Akahori, Hiroshi
Hosaka, Yasuhiro
Akaike, Kaoru
Ikegami, Nobuko
spellingShingle Hernández, Francisco
Akahori, Hiroshi
Hosaka, Yasuhiro
Akaike, Kaoru
Ikegami, Nobuko
A rotary shadow freeze-drying replica method for the three dimensional view of virus ultrastructure
author_facet Hernández, Francisco
Akahori, Hiroshi
Hosaka, Yasuhiro
Akaike, Kaoru
Ikegami, Nobuko
author_sort Hernández, Francisco
description Se describe un método de sombreado rotatorio para preparar réplicas (copias) de viriones estabilizados mediante congelación y secado a baja presión. Esta réplicas permiten el análisis de la ultraestructura viral; empleándose como modelo de estudio la cápside interna de rotavirus humanos. Los hallazgos corroboran el patrón de simetría icosaédrico con un patrón de capsómeros de T=13L, previamente descrito.
title A rotary shadow freeze-drying replica method for the three dimensional view of virus ultrastructure
title_short A rotary shadow freeze-drying replica method for the three dimensional view of virus ultrastructure
title_full A rotary shadow freeze-drying replica method for the three dimensional view of virus ultrastructure
title_fullStr A rotary shadow freeze-drying replica method for the three dimensional view of virus ultrastructure
title_full_unstemmed A rotary shadow freeze-drying replica method for the three dimensional view of virus ultrastructure
title_sort rotary shadow freeze-drying replica method for the three dimensional view of virus ultrastructure
title_alt A rotary shadow freeze-drying replica method for the three dimensional view of virus ultrastructure
publisher Universidad de Costa Rica
publishDate 1994
url https://revistas.ucr.ac.cr/index.php/rbt/article/view/28716
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