Anti-candidal effect of endophytic fungi isolated from Calotropis gigantea
Candida albicans is a most common cause of fungal infections in animals and birds. Understanding the increasing resistance of C. albicans to various antifungal therapeutic agents is important to discover new anti-candidal alternatives. The present study investigated the anti-candidal potential of fi...
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| Formato: | Online |
| Idioma: | eng |
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Universidad de Costa Rica
2017
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| Acceso en línea: | https://revistas.ucr.ac.cr/index.php/rbt/article/view/26269 |
| id |
RBT26269 |
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| record_format |
ojs |
| institution |
Universidad de Costa Rica |
| collection |
Revista de Biología Tropical |
| language |
eng |
| format |
Online |
| author |
Nath, Archana Joshi, Santaram |
| spellingShingle |
Nath, Archana Joshi, Santaram Anti-candidal effect of endophytic fungi isolated from Calotropis gigantea |
| author_facet |
Nath, Archana Joshi, Santaram |
| author_sort |
Nath, Archana |
| description |
Candida albicans is a most common cause of fungal infections in animals and birds. Understanding the increasing resistance of C. albicans to various antifungal therapeutic agents is important to discover new anti-candidal alternatives. The present study investigated the anti-candidal potential of five endophytic fungi extracts, isolated from the tropical ethnoveterinary plant, Calotropis gigantea. We firstly evaluated the in vitro antifungal activities of endophytic fungi extracts by the well diffusion method. Secondly, the cells of C. albicans were treated with the potent extract to observe significant ultrastructural changes. To further investigate the in vivo antifungal activity of the extract, some laboratory experiments with mice were undertaken, and posteriourly, the different organs were studied under the electron microscope for any deformities. Phomopsis asparagi showed the best anti-candidal activity with a minimum inhibitory concentration (MIC) of 46.9 µg/mL. The fungal test pathogen (C. albicans) exhibited various cell deformities when treated with the extract of P. asparagi.Histopathological studies of the vital organs of mice treated with the potent fungal extract did not show any significant pathological conditions when viewed under scanning electron microscope. Thus, P. asparagi can be a potential candidate for anti-candidal agents against C. albicans. Future studies will focus on the isolation of the bioactive components of the extract. |
| title |
Anti-candidal effect of endophytic fungi isolated from Calotropis gigantea |
| title_short |
Anti-candidal effect of endophytic fungi isolated from Calotropis gigantea |
| title_full |
Anti-candidal effect of endophytic fungi isolated from Calotropis gigantea |
| title_fullStr |
Anti-candidal effect of endophytic fungi isolated from Calotropis gigantea |
| title_full_unstemmed |
Anti-candidal effect of endophytic fungi isolated from Calotropis gigantea |
| title_sort |
anti-candidal effect of endophytic fungi isolated from calotropis gigantea |
| title_alt |
Anti-candidal effect of endophytic fungi isolated from Calotropis gigantea |
| publisher |
Universidad de Costa Rica |
| publishDate |
2017 |
| url |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/26269 |
| work_keys_str_mv |
AT natharchana anticandidaleffectofendophyticfungiisolatedfromcalotropisgigantea AT joshisantaram anticandidaleffectofendophyticfungiisolatedfromcalotropisgigantea |
| _version_ |
1810115052235128832 |
| spelling |
RBT262692022-06-09T18:08:02Z Anti-candidal effect of endophytic fungi isolated from Calotropis gigantea Anti-candidal effect of endophytic fungi isolated from Calotropis gigantea Nath, Archana Joshi, Santaram endophytic fungi anti-candidal Candida albicans scanning electron microscope ultrastructure. endophytic fungi anti-candidal Candida albicans scanning electron microscope ultrastructure. Candida albicans is a most common cause of fungal infections in animals and birds. Understanding the increasing resistance of C. albicans to various antifungal therapeutic agents is important to discover new anti-candidal alternatives. The present study investigated the anti-candidalpotential of five endophytic fungi extracts, isolated from the tropical ethnoveterinary plant, Calotropis gigantea. We firstly evaluated the in vitro antifungal activities of endophytic fungi extracts by the well diffusion method. Secondly, the cells of C. albicans were treated with the potent extract to observe significant ultrastructural changes. To further investigate the in vivo antifungal activity of the extract, some laboratory experiments with mice were undertaken, and posteriourly, the different organs were studied under the electron microscope for any deformities. Phomopsis asparagi showed the best anti-candidal activity with a minimum inhibitory concentration (MIC) of 46.9 µg/mL. The fungal test pathogen (C. albicans) exhibited various cell deformities when treated with the extract of P. asparagi. Histopathological studies of the vital organs of mice treated with the potent fungal extract did not show any significant pathological conditions when viewed under scanning electron microscope. Thus, P. asparagi can be a potential candidate for anti-candidal agents against C. albicans. Future studies will focus on the isolation of the bioactive components of the extract. Candida albicans is a most common cause of fungal infections in animals and birds. Understanding the increasing resistance of C. albicans to various antifungal therapeutic agents is important to discover new anti-candidal alternatives. The present study investigated the anti-candidal potential of five endophytic fungi extracts, isolated from the tropical ethnoveterinary plant, Calotropis gigantea. We firstly evaluated the in vitro antifungal activities of endophytic fungi extracts by the well diffusion method. Secondly, the cells of C. albicans were treated with the potent extract to observe significant ultrastructural changes. To further investigate the in vivo antifungal activity of the extract, some laboratory experiments with mice were undertaken, and posteriourly, the different organs were studied under the electron microscope for any deformities. Phomopsis asparagi showed the best anti-candidal activity with a minimum inhibitory concentration (MIC) of 46.9 µg/mL. The fungal test pathogen (C. albicans) exhibited various cell deformities when treated with the extract of P. asparagi.Histopathological studies of the vital organs of mice treated with the potent fungal extract did not show any significant pathological conditions when viewed under scanning electron microscope. Thus, P. asparagi can be a potential candidate for anti-candidal agents against C. albicans. Future studies will focus on the isolation of the bioactive components of the extract. Universidad de Costa Rica 2017-12-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article application/pdf text/html https://revistas.ucr.ac.cr/index.php/rbt/article/view/26269 10.15517/rbt.v65i4.26269 Revista de Biología Tropical; Vol. 65 No. 4 (2017): Volume 65 – Regular number 4 – December 2017; 1437–1447 Revista de Biología Tropical; Vol. 65 Núm. 4 (2017): Volumen 65 – Número regular 4 – Diciembre 2017; 1437–1447 Revista Biología Tropical; Vol. 65 N.º 4 (2017): Volume 65 – Regular number 4 – December 2017; 1437–1447 2215-2075 0034-7744 10.15517/rbt.v65i4 eng https://revistas.ucr.ac.cr/index.php/rbt/article/view/26269/30650 https://revistas.ucr.ac.cr/index.php/rbt/article/view/26269/30651 Copyright (c) 2017 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0 |