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RBT24373
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ojs
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RBT243732022-05-19T18:23:52Z Purification and properties of a coagulant proteinase isolated from bushmaster (Lachesis muta) venom (Serpentes: Viperidae) Purification and properties of a coagulant proteinase isolated from bushmaster (Lachesis muta) venom (Serpentes: Viperidae) Aragón-Ortiz, F. The venom of Lachesis muta is a rich source of a thrombin-like enzyme. Its coagulant proteinase was purified by DEAE -Sephadex A -50 followed by agmatine CH -Sepharose and gel filtration on Sephadex G-100. On polyacrylamide gel electrophoresis at pH 8.4 a single band was observed. Its molecular weight by gel filtration was 49,000. The coagulant and esterolytic activities toward human fibrinogen and Tame of the inudasa were 662 NIH units/mg of protein and 4.37 Δ OD225/min x 10-3 / µg / ml, respectively. These values represent 23 and 5.7 fold increase over the crude venom. The enzyme mudasa, was evaluated with serum from human patients at Hospital Nacional de Niños Dr. Carlos Sáenz Herrera and found to be 21 valuable reagent for the quantification of fibrinogen on heparinized plasma. Universidad de Costa Rica 1986-06-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article application/pdf https://revistas.ucr.ac.cr/index.php/rbt/article/view/24373 Revista de Biología Tropical; Vol. 34 No. 1 (1986): Volume 34 – Regular number 1 – June 1986; 55–58 Revista de Biología Tropical; Vol. 34 Núm. 1 (1986): Volumen 34 – Número regular 1 – Junio 1986; 55–58 Revista Biología Tropical; Vol. 34 N.º 1 (1986): Volume 34 – Regular number 1 – June 1986; 55–58 2215-2075 0034-7744 10.15517/rbt.v34i1 eng https://revistas.ucr.ac.cr/index.php/rbt/article/view/24373/24494 Copyright (c) 1986 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0
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| institution |
Universidad de Costa Rica
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| collection |
Revista de Biología Tropical
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| language |
eng
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| format |
Online
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| author |
Aragón-Ortiz, F.
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| spellingShingle |
Aragón-Ortiz, F.
Purification and properties of a coagulant proteinase isolated from bushmaster (Lachesis muta) venom (Serpentes: Viperidae)
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| author_facet |
Aragón-Ortiz, F.
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| author_sort |
Aragón-Ortiz, F.
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| title |
Purification and properties of a coagulant proteinase isolated from bushmaster (Lachesis muta) venom (Serpentes: Viperidae)
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| title_short |
Purification and properties of a coagulant proteinase isolated from bushmaster (Lachesis muta) venom (Serpentes: Viperidae)
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| title_full |
Purification and properties of a coagulant proteinase isolated from bushmaster (Lachesis muta) venom (Serpentes: Viperidae)
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| title_fullStr |
Purification and properties of a coagulant proteinase isolated from bushmaster (Lachesis muta) venom (Serpentes: Viperidae)
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| title_full_unstemmed |
Purification and properties of a coagulant proteinase isolated from bushmaster (Lachesis muta) venom (Serpentes: Viperidae)
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| title_sort |
purification and properties of a coagulant proteinase isolated from bushmaster (lachesis muta) venom (serpentes: viperidae)
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| title_alt |
Purification and properties of a coagulant proteinase isolated from bushmaster (Lachesis muta) venom (Serpentes: Viperidae)
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| publisher |
Universidad de Costa Rica
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| publishDate |
1986
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| url |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/24373
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| work_keys_str_mv |
AT aragonortizf purificationandpropertiesofacoagulantproteinaseisolatedfrombushmasterlachesismutavenomserpentesviperidae
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| _version_ |
1810114955320492032
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