Characterization of a Lectin-Like protein isolated from Lachesis muta Snake venon
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| Formato: | Online |
| Idioma: | eng |
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Universidad de Costa Rica
1989
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| Acceso en línea: | https://revistas.ucr.ac.cr/index.php/rbt/article/view/23624 |
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RBT23624 |
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RBT236242022-05-20T13:54:43Z Characterization of a Lectin-Like protein isolated from Lachesis muta Snake venon Characterization of a Lectin-Like protein isolated from Lachesis muta Snake venon Aragón-Ortíz, F. Brenes-Brenes, J. R. Gubensek, F. A lectin-like protein was isolated from L. muta venom by gel filtration on BIO Gel P-100 followed by column Chromatography on DEAE-Sephadex A-50. The protein eluted at 0.4 M NaO in 0.01 Tris pH 7.3 and exhibited agglutinin activity toward 0+ human erythrocytes. The protein is a dimer with Mr 28 kDa.Amino acid analysis revealed high content of tryptophan and acid residues and low content of cysteine and methionine residues. No neutral carbohydrates and sialic acid were detected. Circular dichroic spectrum shows 78% of B structure and 1% of α structure. In vitro experiments with ery throcytes from rat, rabbit and dog revealed strong agglutination while red blood cells from mice, sheep and goat were not agglutinated. In vivo experiments using non-anesthetized rats, a sharp and prolonged fall in the blood pressure was observed at protein dose of 1.5 mg/kg. Double dose of protein caused the death of the animal. Universidad de Costa Rica 1989-06-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article application/pdf https://revistas.ucr.ac.cr/index.php/rbt/article/view/23624 Revista de Biología Tropical; Vol. 37 No. 1 (1989): Volume 37 – Regular number 1 – June 1989; 79–83 Revista de Biología Tropical; Vol. 37 Núm. 1 (1989): Volumen 37 – Número regular 1 – Junio 1989; 79–83 Revista Biología Tropical; Vol. 37 N.º 1 (1989): Volume 37 – Regular number 1 – June 1989; 79–83 2215-2075 0034-7744 10.15517/rbt.v37i1 eng https://revistas.ucr.ac.cr/index.php/rbt/article/view/23624/23823 Copyright (c) 1989 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0 |
| institution |
Universidad de Costa Rica |
| collection |
Revista de Biología Tropical |
| language |
eng |
| format |
Online |
| author |
Aragón-Ortíz, F. Brenes-Brenes, J. R. Gubensek, F. |
| spellingShingle |
Aragón-Ortíz, F. Brenes-Brenes, J. R. Gubensek, F. Characterization of a Lectin-Like protein isolated from Lachesis muta Snake venon |
| author_facet |
Aragón-Ortíz, F. Brenes-Brenes, J. R. Gubensek, F. |
| author_sort |
Aragón-Ortíz, F. |
| title |
Characterization of a Lectin-Like protein isolated from Lachesis muta Snake venon |
| title_short |
Characterization of a Lectin-Like protein isolated from Lachesis muta Snake venon |
| title_full |
Characterization of a Lectin-Like protein isolated from Lachesis muta Snake venon |
| title_fullStr |
Characterization of a Lectin-Like protein isolated from Lachesis muta Snake venon |
| title_full_unstemmed |
Characterization of a Lectin-Like protein isolated from Lachesis muta Snake venon |
| title_sort |
characterization of a lectin-like protein isolated from lachesis muta snake venon |
| title_alt |
Characterization of a Lectin-Like protein isolated from Lachesis muta Snake venon |
| publisher |
Universidad de Costa Rica |
| publishDate |
1989 |
| url |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/23624 |
| work_keys_str_mv |
AT aragonortizf characterizationofalectinlikeproteinisolatedfromlachesismutasnakevenon AT brenesbrenesjr characterizationofalectinlikeproteinisolatedfromlachesismutasnakevenon AT gubensekf characterizationofalectinlikeproteinisolatedfromlachesismutasnakevenon |
| _version_ |
1810114920925102080 |