Properties and N-terminal amino acid sequences of three Erythrina lectins from Costa Rica (Leguminosae)

Three Erythrina lectins, from E. fusca, E. globocaliz and E. costaricensis were isolated by affinity chromatography. The lectins are glycoproteins with a monomenc molecular weight of 28 000. They agglutinate human erythrocytes irrespective of blood type and the activity was inhibited by N-acetyl-gal...

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Autores principales:	Aragón-Ortiz, Federico, Nanne-Echandi, Clara I, Fink, Edwin
Formato:	Online
Idioma:	eng
Publicado:	Universidad de Costa Rica 1994
Acceso en línea:	https://revistas.ucr.ac.cr/index.php/rbt/article/view/23222

id	RBT23222
record_format	ojs
spelling	RBT232222022-05-20T14:43:23Z Properties and N-terminal amino acid sequences of three Erythrina lectins from Costa Rica (Leguminosae) Properties and N-terminal amino acid sequences of three Erythrina lectins from Costa Rica (Leguminosae) Aragón-Ortiz, Federico Nanne-Echandi, Clara I Fink, Edwin Three Erythrina lectins, from E. fusca, E. globocaliz and E. costaricensis were isolated by affinity chromatography. The lectins are glycoproteins with a monomenc molecular weight of 28 000. They agglutinate human erythrocytes irrespective of blood type and the activity was inhibited by N-acetyl-galactosamine, galactose and lactose. The N-terminal amino acid sequences of the three lectins were deterrnined by automated Edman degradation of the native proteins. Comparison with the sequences of ten other legume lectins revealed extensive homology. The N-terminal amino acid for E.fusca is Val, and Ala for E. globocaliz and E. costaricensis. Three Erythrina lectins, from E. fusca, E. globocaliz and E. costaricensis were isolated by affinity chromatography. The lectins are glycoproteins with a monomenc molecular weight of 28 000. They agglutinate human erythrocytes irrespective of blood type and the activity was inhibited by N-acetyl-galactosamine, galactose and lactose. The N-terminal amino acid sequences of the three lectins were deterrnined by automated Edman degradation of the native proteins. Comparison with the sequences of ten other legume lectins revealed extensive homology. The N-terminal amino acid for E.fusca is Val, and Ala for E. globocaliz and E. costaricensis. Universidad de Costa Rica 1994-12-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article application/pdf https://revistas.ucr.ac.cr/index.php/rbt/article/view/23222 Revista de Biología Tropical; Vol. 42 No. 3 (1994): Volume 42 – Regular number 3 – December 1994; 467–471 Revista de Biología Tropical; Vol. 42 Núm. 3 (1994): Volumen 42 – Número regular 3 – Diciembre 1994; 467–471 Revista Biología Tropical; Vol. 42 N.º 3 (1994): Volume 42 – Regular number 3 – December 1994; 467–471 2215-2075 0034-7744 eng https://revistas.ucr.ac.cr/index.php/rbt/article/view/23222/23558 Copyright (c) 1994 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0
institution	Universidad de Costa Rica
collection	Revista de Biología Tropical
language	eng
format	Online
author	Aragón-Ortiz, Federico Nanne-Echandi, Clara I Fink, Edwin
spellingShingle	Aragón-Ortiz, Federico Nanne-Echandi, Clara I Fink, Edwin Properties and N-terminal amino acid sequences of three Erythrina lectins from Costa Rica (Leguminosae)
author_facet	Aragón-Ortiz, Federico Nanne-Echandi, Clara I Fink, Edwin
author_sort	Aragón-Ortiz, Federico
description	Three Erythrina lectins, from E. fusca, E. globocaliz and E. costaricensis were isolated by affinity chromatography. The lectins are glycoproteins with a monomenc molecular weight of 28 000. They agglutinate human erythrocytes irrespective of blood type and the activity was inhibited by N-acetyl-galactosamine, galactose and lactose. The N-terminal amino acid sequences of the three lectins were deterrnined by automated Edman degradation of the native proteins. Comparison with the sequences of ten other legume lectins revealed extensive homology. The N-terminal amino acid for E.fusca is Val, and Ala for E. globocaliz and E. costaricensis.
title	Properties and N-terminal amino acid sequences of three Erythrina lectins from Costa Rica (Leguminosae)
title_short	Properties and N-terminal amino acid sequences of three Erythrina lectins from Costa Rica (Leguminosae)
title_full	Properties and N-terminal amino acid sequences of three Erythrina lectins from Costa Rica (Leguminosae)
title_fullStr	Properties and N-terminal amino acid sequences of three Erythrina lectins from Costa Rica (Leguminosae)
title_full_unstemmed	Properties and N-terminal amino acid sequences of three Erythrina lectins from Costa Rica (Leguminosae)
title_sort	properties and n-terminal amino acid sequences of three erythrina lectins from costa rica (leguminosae)
title_alt	Properties and N-terminal amino acid sequences of three Erythrina lectins from Costa Rica (Leguminosae)
publisher	Universidad de Costa Rica
publishDate	1994
url	https://revistas.ucr.ac.cr/index.php/rbt/article/view/23222
work_keys_str_mv	AT aragonortizfederico propertiesandnterminalaminoacidsequencesofthreeerythrinalectinsfromcostaricaleguminosae AT nanneechandiclarai propertiesandnterminalaminoacidsequencesofthreeerythrinalectinsfromcostaricaleguminosae AT finkedwin propertiesandnterminalaminoacidsequencesofthreeerythrinalectinsfromcostaricaleguminosae
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Properties and N-terminal amino acid sequences of three Erythrina lectins from Costa Rica (Leguminosae)

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