In vitro plant regeneration system for tropical butternut squash genotypes (Cucurbita moschata)

An efficient and reproducible method for regeneration of commercial and pure lines of tropical butternut squash (Cucurbita moschata) plants via somatic embryogenesis was developed. The influence of genotype, explant source, N6-benzylaminopurine (BAP), 2,4-dichlorophenoxyacetic acid (2,4-D) and 2,4,5...

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Autores principales:	Valdez-Melara, Marta, García, Alexander, Delgado, Marlon, Gatica-Arias, Andrés M, Ramírez-Fonseca, Pilar
Formato:	Online
Idioma:	eng
Publicado:	Universidad de Costa Rica 2009
Acceso en línea:	https://revistas.ucr.ac.cr/index.php/rbt/article/view/21279

id	RBT21279
record_format	ojs
spelling	RBT212792022-06-06T18:29:01Z In vitro plant regeneration system for tropical butternut squash genotypes (Cucurbita moschata) In vitro plant regeneration system for tropical butternut squash genotypes (Cucurbita moschata) Valdez-Melara, Marta García, Alexander Delgado, Marlon Gatica-Arias, Andrés M Ramírez-Fonseca, Pilar cucurbits cucurbita moschata auxins cytokinin explant source genotype Costa Rica cucurbits cucurbita moschata auxins cytokinin explant source genotype Costa Rica An efficient and reproducible method for regeneration of commercial and pure lines of tropical butternut squash (Cucurbita moschata) plants via somatic embryogenesis was developed. The influence of genotype, explant source, N6-benzylaminopurine (BAP), 2,4-dichlorophenoxyacetic acid (2,4-D) and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) concentration on somatic embryogenesis induction was investigated. Friable embryogenic calli was produced from zigotic embryos (53-56%) and cotyledons from seedlings (70%) of C. moschata cv. Sello de Oro cultured on callus induction medium (CIM) supplemented with 0.5 mg/l or 3.5 mg/l 2,4-D. No embryogenic calli was obtained from leaf segments of C. moschata cv. Sello de Oro cultured on CIM supplemented with different concentrations of BAP and 2,4-D and cotyledons from seedlings of C. moschata cv. PVG 04 cultured on CIM with BAP and 2,4,5-T. Embryogenic calli induction was achieved in 75% C. moschata pure lines evaluated and calli percentage frequency range from 5% to 34%. Successful acclimatization of squash in vitro plants was achieved in the greenhouse and in the field. Regenerated plants appeared morphologically normal and set flowers and fruits with seeds that could germinate normally. An efficient and reproducible method for regeneration of commercial and pure lines of tropical butternut squash (Cucurbita moschata) plants via somatic embryogenesis was developed. The influence of genotype, explant source, N6-benzylaminopurine (BAP), 2,4-dichlorophenoxyacetic acid (2,4-D) and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) concentration on somatic embryogenesis induction was investigated. Friable embryogenic calli was produced from zigotic embryos (53-56%) and cotyledons from seedlings (70%) of C. moschata cv. Sello de Oro cultured on callus induction medium (CIM) supplemented with 0.5 mg/l or 3.5 mg/l 2,4-D. No embryogenic calli was obtained from leaf segments of C. moschata cv. Sello de Oro cultured on CIM supplemented with different concentrations of BAP and 2,4-D and cotyledons from seedlings of C. moschata cv. PVG 04 cultured on CIM with BAP and 2,4,5-T. Embryogenic calli induction was achieved in 75% C. moschata pure lines evaluated and calli percentage frequency range from 5% to 34%. Successful acclimatization of squash in vitro plants was achieved in the greenhouse and in the field. Regenerated plants appeared morphologically normal and set flowers and fruits with seeds that could germinate normally. Universidad de Costa Rica 2009-11-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article application/pdf https://revistas.ucr.ac.cr/index.php/rbt/article/view/21279 10.15517/rbt.v57i0.21279 Revista de Biología Tropical; Vol. 57 No. S1 (2009): Volume 57 – Supplement 1 – November 2009: 50th anniversary School of Biology, University of Costa Rica; 119–127 Revista de Biología Tropical; Vol. 57 Núm. S1 (2009): Volumen 57 – Suplemento 1 – Noviembre: 50 aniversario Escuela de Biología, Universidad de Costa; 119–127 Revista Biología Tropical; Vol. 57 N.º S1 (2009): Volume 57 – Supplement 1 – November 2009: 50th anniversary School of Biology, University of Costa Rica; 119–127 2215-2075 0034-7744 10.15517/rbt.v57i0 eng https://revistas.ucr.ac.cr/index.php/rbt/article/view/21279/21503 Copyright (c) 2009 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0
institution	Universidad de Costa Rica
collection	Revista de Biología Tropical
language	eng
format	Online
author	Valdez-Melara, Marta García, Alexander Delgado, Marlon Gatica-Arias, Andrés M Ramírez-Fonseca, Pilar
spellingShingle	Valdez-Melara, Marta García, Alexander Delgado, Marlon Gatica-Arias, Andrés M Ramírez-Fonseca, Pilar In vitro plant regeneration system for tropical butternut squash genotypes (Cucurbita moschata)
author_facet	Valdez-Melara, Marta García, Alexander Delgado, Marlon Gatica-Arias, Andrés M Ramírez-Fonseca, Pilar
author_sort	Valdez-Melara, Marta
description	An efficient and reproducible method for regeneration of commercial and pure lines of tropical butternut squash (Cucurbita moschata) plants via somatic embryogenesis was developed. The influence of genotype, explant source, N6-benzylaminopurine (BAP), 2,4-dichlorophenoxyacetic acid (2,4-D) and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) concentration on somatic embryogenesis induction was investigated. Friable embryogenic calli was produced from zigotic embryos (53-56%) and cotyledons from seedlings (70%) of C. moschata cv. Sello de Oro cultured on callus induction medium (CIM) supplemented with 0.5 mg/l or 3.5 mg/l 2,4-D. No embryogenic calli was obtained from leaf segments of C. moschata cv. Sello de Oro cultured on CIM supplemented with different concentrations of BAP and 2,4-D and cotyledons from seedlings of C. moschata cv. PVG 04 cultured on CIM with BAP and 2,4,5-T. Embryogenic calli induction was achieved in 75% C. moschata pure lines evaluated and calli percentage frequency range from 5% to 34%. Successful acclimatization of squash in vitro plants was achieved in the greenhouse and in the field. Regenerated plants appeared morphologically normal and set flowers and fruits with seeds that could germinate normally.
title	In vitro plant regeneration system for tropical butternut squash genotypes (Cucurbita moschata)
title_short	In vitro plant regeneration system for tropical butternut squash genotypes (Cucurbita moschata)
title_full	In vitro plant regeneration system for tropical butternut squash genotypes (Cucurbita moschata)
title_fullStr	In vitro plant regeneration system for tropical butternut squash genotypes (Cucurbita moschata)
title_full_unstemmed	In vitro plant regeneration system for tropical butternut squash genotypes (Cucurbita moschata)
title_sort	in vitro plant regeneration system for tropical butternut squash genotypes (cucurbita moschata)
title_alt	In vitro plant regeneration system for tropical butternut squash genotypes (Cucurbita moschata)
publisher	Universidad de Costa Rica
publishDate	2009
url	https://revistas.ucr.ac.cr/index.php/rbt/article/view/21279
work_keys_str_mv	AT valdezmelaramarta invitroplantregenerationsystemfortropicalbutternutsquashgenotypescucurbitamoschata AT garciaalexander invitroplantregenerationsystemfortropicalbutternutsquashgenotypescucurbitamoschata AT delgadomarlon invitroplantregenerationsystemfortropicalbutternutsquashgenotypescucurbitamoschata AT gaticaariasandresm invitroplantregenerationsystemfortropicalbutternutsquashgenotypescucurbitamoschata AT ramirezfonsecapilar invitroplantregenerationsystemfortropicalbutternutsquashgenotypescucurbitamoschata
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In vitro plant regeneration system for tropical butternut squash genotypes (Cucurbita moschata)

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