The inhibitory effect of a non-yessotoxin-producing dinoflagellate, Lingulodinium polyedrum (Stein) Dodge, towards Vibrio vulnificus and Staphylococcus aureus
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| Formato: | Online |
| Idioma: | eng |
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Universidad de Costa Rica
2016
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| Acceso en línea: | https://revistas.ucr.ac.cr/index.php/rbt/article/view/19320 |
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RBT19320 |
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RBT193202022-06-09T17:53:53Z The inhibitory effect of a non-yessotoxin-producing dinoflagellate, Lingulodinium polyedrum (Stein) Dodge, towards Vibrio vulnificus and Staphylococcus aureus Quijano-Scheggia, Sonia Barajas-Gonzalez, Maribel Chang Lim, Hong Pin Leaw, Chui Olivos-Ortiz, Aramis Gaviño-Rodriguez, Juan Blanco Pérez, Juan Bates, Stephen S. Proliferaciones algales antibiótico resistencia bacteriana Lingulodinium polyedrum fitoplancton Staphylococcus aureus Vibrio vulnificus yessotoxina. The increased bacterial resistance to antibiotics has caused global concern, prompting the search for new compounds. Because of their abundance and diversity, marine phytoplankton is an important potential source of such compounds. Research on dinoflagellates has led to the discovery of inhibitors of bacterial growth. The marine dinoflagellate Lingulodinium polyedrum blooms in different regions of the world, including Mexico, and is also known to regulate the growth of other species in coastal waters. Here, we investigated the taxonomy of this dinoflagellate and characterized the ability of its extracts to inhibit the growth of two bacteria of medical importance (Vibrio vulnificus and Staphylococcus aureus). Taxonomic characterization was performed by PCR and gene amplification of ITS, and confirmed that the species isolated off the Pacific coast of Mexico was L. polyedrum. To prove the inhibitory effect of L. polyedrum extracts, cultures were harvested by centrifugation. Pellets from three cellular abundances were extracted with water, methanol, hexane and chloroform. The experiments on V. vulnificus showed a high growth inhibition for the four extracts, ranging from 77 to 98 %. Surprisingly, the growth inhibition was lower when the extracts originated from a higher L. polyedrum cell abundance, ranging from 0 to 34 %. For S. aureus, the growth inhibition was also high, but not statistically different for all extracts and cell abundances, ranging from 62 to 99 %. This study obtained promising results for future pharmacological applications. Our Mexican strain of L. polyedrum did not produce any detectable yessotoxins. Universidad de Costa Rica 2016-06-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Contribution application/pdf text/html application/pdf https://revistas.ucr.ac.cr/index.php/rbt/article/view/19320 10.15517/rbt.v64i2.19320 Revista de Biología Tropical; Vol. 64 No. 2 (2016): Volume 64 – Regular number 2 – June 2016; 805–816 Revista de Biología Tropical; Vol. 64 Núm. 2 (2016): Volumen 64 – Número regular 2 – Junio 2016; 805–816 Revista Biología Tropical; Vol. 64 N.º 2 (2016): Volume 64 – Regular number 2 – June 2016; 805–816 2215-2075 0034-7744 10.15517/rbt.v64i2 eng https://revistas.ucr.ac.cr/index.php/rbt/article/view/19320/24575 https://revistas.ucr.ac.cr/index.php/rbt/article/view/19320/24576 https://revistas.ucr.ac.cr/index.php/rbt/article/view/19320/34894 Copyright (c) 2016 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0 |
| institution |
Universidad de Costa Rica |
| collection |
Revista de Biología Tropical |
| language |
eng |
| format |
Online |
| author |
Quijano-Scheggia, Sonia Barajas-Gonzalez, Maribel Chang Lim, Hong Pin Leaw, Chui Olivos-Ortiz, Aramis Gaviño-Rodriguez, Juan Blanco Pérez, Juan Bates, Stephen S. |
| spellingShingle |
Quijano-Scheggia, Sonia Barajas-Gonzalez, Maribel Chang Lim, Hong Pin Leaw, Chui Olivos-Ortiz, Aramis Gaviño-Rodriguez, Juan Blanco Pérez, Juan Bates, Stephen S. The inhibitory effect of a non-yessotoxin-producing dinoflagellate, Lingulodinium polyedrum (Stein) Dodge, towards Vibrio vulnificus and Staphylococcus aureus |
| author_facet |
Quijano-Scheggia, Sonia Barajas-Gonzalez, Maribel Chang Lim, Hong Pin Leaw, Chui Olivos-Ortiz, Aramis Gaviño-Rodriguez, Juan Blanco Pérez, Juan Bates, Stephen S. |
| author_sort |
Quijano-Scheggia, Sonia |
| title |
The inhibitory effect of a non-yessotoxin-producing dinoflagellate, Lingulodinium polyedrum (Stein) Dodge, towards Vibrio vulnificus and Staphylococcus aureus |
| title_short |
The inhibitory effect of a non-yessotoxin-producing dinoflagellate, Lingulodinium polyedrum (Stein) Dodge, towards Vibrio vulnificus and Staphylococcus aureus |
| title_full |
The inhibitory effect of a non-yessotoxin-producing dinoflagellate, Lingulodinium polyedrum (Stein) Dodge, towards Vibrio vulnificus and Staphylococcus aureus |
| title_fullStr |
The inhibitory effect of a non-yessotoxin-producing dinoflagellate, Lingulodinium polyedrum (Stein) Dodge, towards Vibrio vulnificus and Staphylococcus aureus |
| title_full_unstemmed |
The inhibitory effect of a non-yessotoxin-producing dinoflagellate, Lingulodinium polyedrum (Stein) Dodge, towards Vibrio vulnificus and Staphylococcus aureus |
| title_sort |
inhibitory effect of a non-yessotoxin-producing dinoflagellate, lingulodinium polyedrum (stein) dodge, towards vibrio vulnificus and staphylococcus aureus |
| publisher |
Universidad de Costa Rica |
| publishDate |
2016 |
| url |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/19320 |
| work_keys_str_mv |
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1810114787995025408 |