Inclusion bodies induced by Bean rugose mosaic comovirus seen under light microscopy

Se observaron dos tipos de inclusiones virales, mediante microscopia de luz, en hojas de plantas de frijol (Phaseolus vulgaris) previamente infectadas con el virus del mosaico rugoso del frijol (“Bean rugose mosaic virus”, BRMV), especie del género Comovirus, familia Comoviridae. Se hallaron inclusi...

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Main Authors:	Rivera, Carmen, Moreira Carmona, Lisela
Format:	Online
Language:	eng
Published:	Universidad de Costa Rica 2000
Online Access:	https://revistas.ucr.ac.cr/index.php/rbt/article/view/18986

id	RBT18986
record_format	ojs
spelling	RBT189862022-06-13T19:50:54Z Inclusion bodies induced by Bean rugose mosaic comovirus seen under light microscopy Inclusion bodies induced by Bean rugose mosaic comovirus seen under light microscopy Rivera, Carmen Moreira Carmona, Lisela comovirus cytopathology light microscopy diagnosis bean Two types of inclusion bodies were consistently observed under light microscopy in bean (Phaseolus vulgaris) leaf tissue infected with Bean rugose mosaic virus (BRMV), a species of the genus Comovirus, family Comoviridae. One type consisted of vacuolated inclusions found mainly in the cytoplasm of epidermal cells. The other type consisted of abundant crystalloid inclusions of different sizes and shapes found consistently in glandular hairs, guard cells, phloem tissue, xylem elements and occasionally in epidermal and mesophyll tissues. The two types of inclusion bodies stained with Azure A and Luxol Brilliant Green Bl-Calcomine Orange 2RS (O-G), and were similar to those seen to be caused by other species of comoviruses. Se observaron dos tipos de inclusiones virales, mediante microscopia de luz, en hojas de plantas de frijol (Phaseolus vulgaris) previamente infectadas con el virus del mosaico rugoso del frijol (“Bean rugose mosaic virus”, BRMV), especie del género Comovirus, familia Comoviridae. Se hallaron inclusiones vesiculadas, principalmente en el citoplasma de células de la epidermis, y abundantes inclusiones cristalinas de diferentes formas y tamaños siempre en células guarda, tricomas glandulares, floema, elementos del xilema y ocasionalmente en células epidérmicas y del mesófilo. Ambos tipos de inclusiones tiñeron con Azure A y con la tinción, verde naranja (Luxol Brilliant Green BL-Calcomine Orange 2 RS) conocida como OG, y son similares a las inclusiones inducidas por otras especies del género Comovirus. Universidad de Costa Rica 2000-12-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article application/pdf https://revistas.ucr.ac.cr/index.php/rbt/article/view/18986 10.15517/rbt.v48i4.18986 Revista de Biología Tropical; Vol. 48 No. 4 (2000): Volume 48 – Regular number 4 – December 2000; 955–959 Revista de Biología Tropical; Vol. 48 Núm. 4 (2000): Volumen 48 – Numero regular 4 – Diciembre 2000; 955–959 Revista Biología Tropical; Vol. 48 N.º 4 (2000): Volume 48 – Regular number 4 – December 2000; 955–959 2215-2075 0034-7744 10.15517/rbt.v48i4 eng https://revistas.ucr.ac.cr/index.php/rbt/article/view/18986/19081 Copyright (c) 2000 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0
institution	Universidad de Costa Rica
collection	Revista de Biología Tropical
language	eng
format	Online
author	Rivera, Carmen Moreira Carmona, Lisela
spellingShingle	Rivera, Carmen Moreira Carmona, Lisela Inclusion bodies induced by Bean rugose mosaic comovirus seen under light microscopy
author_facet	Rivera, Carmen Moreira Carmona, Lisela
author_sort	Rivera, Carmen
description	Se observaron dos tipos de inclusiones virales, mediante microscopia de luz, en hojas de plantas de frijol (Phaseolus vulgaris) previamente infectadas con el virus del mosaico rugoso del frijol (“Bean rugose mosaic virus”, BRMV), especie del género Comovirus, familia Comoviridae. Se hallaron inclusiones vesiculadas, principalmente en el citoplasma de células de la epidermis, y abundantes inclusiones cristalinas de diferentes formas y tamaños siempre en células guarda, tricomas glandulares, floema, elementos del xilema y ocasionalmente en células epidérmicas y del mesófilo. Ambos tipos de inclusiones tiñeron con Azure A y con la tinción, verde naranja (Luxol Brilliant Green BL-Calcomine Orange 2 RS) conocida como OG, y son similares a las inclusiones inducidas por otras especies del género Comovirus.
title	Inclusion bodies induced by Bean rugose mosaic comovirus seen under light microscopy
title_short	Inclusion bodies induced by Bean rugose mosaic comovirus seen under light microscopy
title_full	Inclusion bodies induced by Bean rugose mosaic comovirus seen under light microscopy
title_fullStr	Inclusion bodies induced by Bean rugose mosaic comovirus seen under light microscopy
title_full_unstemmed	Inclusion bodies induced by Bean rugose mosaic comovirus seen under light microscopy
title_sort	inclusion bodies induced by bean rugose mosaic comovirus seen under light microscopy
title_alt	Inclusion bodies induced by Bean rugose mosaic comovirus seen under light microscopy
publisher	Universidad de Costa Rica
publishDate	2000
url	https://revistas.ucr.ac.cr/index.php/rbt/article/view/18986
work_keys_str_mv	AT riveracarmen inclusionbodiesinducedbybeanrugosemosaiccomovirusseenunderlightmicroscopy AT moreiracarmonalisela inclusionbodiesinducedbybeanrugosemosaiccomovirusseenunderlightmicroscopy
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Inclusion bodies induced by Bean rugose mosaic comovirus seen under light microscopy

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