Hypotensive action of an aqueous extract of Pimenta dioica (Myrtaceae) in rats

The intra-venous (i.v.) hypotensive action of the final aqueous fraction of Pimenta dioica was studied in Spontaneously Hypertensive Rats (SHR). The rats were anaesthetized (sodium pentobarbital 50 mg/kg), the trachea, right carotid artery and jugular vein were cannulated for adequate ventilation, d...

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Autores principales:	Suárez, Adriana, Ulate, Guido, Ciccio, José F.
Formato:	Online
Idioma:	eng
Publicado:	Universidad de Costa Rica 2000
Acceso en línea:	https://revistas.ucr.ac.cr/index.php/rbt/article/view/18150

id	RBT18150
record_format	ojs
spelling	RBT181502022-06-13T19:47:42Z Hypotensive action of an aqueous extract of Pimenta dioica (Myrtaceae) in rats Hypotensive action of an aqueous extract of Pimenta dioica (Myrtaceae) in rats Suárez, Adriana Ulate, Guido Ciccio, José F. pimenta dioica traditional medicine herbal medicine antihypertensive effect arterial hypertension pimenta dioica traditional medicine herbal medicine antihypertensive effect arterial hypertension The intra-venous (i.v.) hypotensive action of the final aqueous fraction of Pimenta dioica was studied in Spontaneously Hypertensive Rats (SHR). The rats were anaesthetized (sodium pentobarbital 50 mg/kg), the trachea, right carotid artery and jugular vein were cannulated for adequate ventilation, direct blood pressure measurement and intra-venous adrninistration of extracts, solutions and drugs. The arterial line was connected to a pressure transducer (Viggo-Spectramed model P23 XL) and a polygraph (Grass model 7H) and monitored continuously during the first five minutes afier plant extract adrninistration and then at 5 JlBd 15 minute intervals for one hour. Responses were taken as the maximum pressure changes observed during this periodo Increasing doses of the final aqueous fraction were given Lv. to groups of six SHR each. It produced a dose dependent decrease in blood pressure and the EDso was 45 mg/kg. To discard that the hypotensive effect of the extracts was due to its ionic composition, a solution containing KCI, NaCI, CaC12 and MgCl2 equivalent to the ion contents present in a dose of 50 mg/kg of total aqueous extract was injected to Sprague-Dawley rats (SDN) using the same method as described aboye. It did not produce significant changes in blood pressure. Pharmacological antagonistic studies were done injecting either autonomic ganglion, IX adrenoceptor, The intra-venous (i.v.) hypotensive action of the final aqueous fraction of Pimenta dioica was studied in Spontaneously Hypertensive Rats (SHR). The rats were anaesthetized (sodium pentobarbital 50 mg/kg), the trachea, right carotid artery and jugular vein were cannulated for adequate ventilation, direct blood pressure measurement and intra-venous adrninistration of extracts, solutions and drugs. The arterial line was connected to a pressure transducer (Viggo-Spectramed model P23 XL) and a polygraph (Grass model 7H) and monitored continuously during the first five minutes afier plant extract adrninistration and then at 5 JlBd 15 minute intervals for one hour. Responses were taken as the maximum pressure changes observed during this periodo Increasing doses of the final aqueous fraction were given Lv. to groups of six SHR each. It produced a dose dependent decrease in blood pressure and the EDso was 45 mg/kg. To discard that the hypotensive effect of the extracts was due to its ionic composition, a solution containing KCI, NaCI, CaC12 and MgCl2 equivalent to the ion contents present in a dose of 50 mg/kg of total aqueous extract was injected to Sprague-Dawley rats (SDN) using the same method as described aboye. It did not produce significant changes in blood pressure. Pharmacological antagonistic studies were done injecting either autonomic ganglion, IX adrenoceptor, Universidad de Costa Rica 2000-03-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article application/pdf https://revistas.ucr.ac.cr/index.php/rbt/article/view/18150 10.15517/rbt.v48i1.18150 Revista de Biología Tropical; Vol. 48 No. 1 (2000): Volume 48 – Regular number 1 – March 2000; 53–58 Revista de Biología Tropical; Vol. 48 Núm. 1 (2000): Volumen 48 – Número regular 1 – Marzo 2000; 53–58 Revista Biología Tropical; Vol. 48 N.º 1 (2000): Volume 48 – Regular number 1 – March 2000; 53–58 2215-2075 0034-7744 10.15517/rbt.v48i1 eng https://revistas.ucr.ac.cr/index.php/rbt/article/view/18150/18385 Copyright (c) 2000 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0
institution	Universidad de Costa Rica
collection	Revista de Biología Tropical
language	eng
format	Online
author	Suárez, Adriana Ulate, Guido Ciccio, José F.
spellingShingle	Suárez, Adriana Ulate, Guido Ciccio, José F. Hypotensive action of an aqueous extract of Pimenta dioica (Myrtaceae) in rats
author_facet	Suárez, Adriana Ulate, Guido Ciccio, José F.
author_sort	Suárez, Adriana
description	The intra-venous (i.v.) hypotensive action of the final aqueous fraction of Pimenta dioica was studied in Spontaneously Hypertensive Rats (SHR). The rats were anaesthetized (sodium pentobarbital 50 mg/kg), the trachea, right carotid artery and jugular vein were cannulated for adequate ventilation, direct blood pressure measurement and intra-venous adrninistration of extracts, solutions and drugs. The arterial line was connected to a pressure transducer (Viggo-Spectramed model P23 XL) and a polygraph (Grass model 7H) and monitored continuously during the first five minutes afier plant extract adrninistration and then at 5 JlBd 15 minute intervals for one hour. Responses were taken as the maximum pressure changes observed during this periodo Increasing doses of the final aqueous fraction were given Lv. to groups of six SHR each. It produced a dose dependent decrease in blood pressure and the EDso was 45 mg/kg. To discard that the hypotensive effect of the extracts was due to its ionic composition, a solution containing KCI, NaCI, CaC12 and MgCl2 equivalent to the ion contents present in a dose of 50 mg/kg of total aqueous extract was injected to Sprague-Dawley rats (SDN) using the same method as described aboye. It did not produce significant changes in blood pressure. Pharmacological antagonistic studies were done injecting either autonomic ganglion, IX adrenoceptor,
title	Hypotensive action of an aqueous extract of Pimenta dioica (Myrtaceae) in rats
title_short	Hypotensive action of an aqueous extract of Pimenta dioica (Myrtaceae) in rats
title_full	Hypotensive action of an aqueous extract of Pimenta dioica (Myrtaceae) in rats
title_fullStr	Hypotensive action of an aqueous extract of Pimenta dioica (Myrtaceae) in rats
title_full_unstemmed	Hypotensive action of an aqueous extract of Pimenta dioica (Myrtaceae) in rats
title_sort	hypotensive action of an aqueous extract of pimenta dioica (myrtaceae) in rats
title_alt	Hypotensive action of an aqueous extract of Pimenta dioica (Myrtaceae) in rats
publisher	Universidad de Costa Rica
publishDate	2000
url	https://revistas.ucr.ac.cr/index.php/rbt/article/view/18150
work_keys_str_mv	AT suarezadriana hypotensiveactionofanaqueousextractofpimentadioicamyrtaceaeinrats AT ulateguido hypotensiveactionofanaqueousextractofpimentadioicamyrtaceaeinrats AT cicciojosef hypotensiveactionofanaqueousextractofpimentadioicamyrtaceaeinrats
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Hypotensive action of an aqueous extract of Pimenta dioica (Myrtaceae) in rats

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