Effect of 2,4-D, hydric stress and light on indica rice (Oryza sativa) somatic embryogenesis

Effect of 2,4-D, hydric stress and light on indica rice (Oryza sativa) somatic embryogenesis

With the purpose of increasing the embryogenesis regeneration process in vitroplants obtained from somatic embryos of the indica rice variety CR-5272 (Oryza sativa L.), two independent experiments were performed. The first experiment consisted in the effect of combination of three concentrations of...

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Autores principales: Meneses, Allan, Flores, Dora, Muñoz, Miguel, Arrieta, Griselda, Espinoza, Ana M
Formato: Online
Idioma:eng
Publicado: Universidad de Costa Rica 2005
Acceso en línea:https://revistas.ucr.ac.cr/index.php/rbt/article/view/14598
id RBT14598
record_format ojs
spelling RBT145982022-05-30T17:45:40Z Effect of 2,4-D, hydric stress and light on indica rice (Oryza sativa) somatic embryogenesis Effect of 2,4-D, hydric stress and light on indica rice (Oryza sativa) somatic embryogenesis Meneses, Allan Flores, Dora Muñoz, Miguel Arrieta, Griselda Espinoza, Ana M rice somatic embryogenesis light plant regeneration Rice somatic embryogenesis light plant regeneration With the purpose of increasing the embryogenesis regeneration process in vitroplants obtained from somatic embryos of the indica rice variety CR-5272 (Oryza sativa L.), two independent experiments were performed. The first experiment consisted in the effect of combination of three concentrations of the gelling agent Phytagel (1.8, 2.4, and 3 gL-1) and four 2,4-D concentrations (2.26, 4.52, 6.78, and 9.05 M) on the induction and subsequent regeneration of embryogenic calli. On the second experiment, the pre-regeneration phase was modified; calli were subjected to darkness or diffuse light conditions for one, two, and three weeks. In embryogenesis induction, 35% calligenesis was obtained using the MS culture medium supplemented with 6.78 M of 2,4-D and 2.4 gL-1 Phytagel , whereas on the control treatment (MS medium supplemented with 9.05 M of 2,4-D and 3 gL-1 Phytagel ) 24% calligenesis was obtained. In addition, regeneration percentages were improved (22% and 16% for calli induced with the above treatments, respectively). Furthermore, in light exposure experiments, the best result was obtained by exposing the embryogenic calli to darkness for one week in pre-regeneration, followed by direct light exposure during the regeneration phase. With the purpose of increasing the embryogenesis regeneration process in vitroplants obtained from somatic embryos of the indica rice variety CR-5272 (Oryza sativa L.), two independent experiments were performed. The first experiment consisted in the effect of combination of three concentrations of the gelling agent Phytagel (1.8, 2.4, and 3 gL-1) and four 2,4-D concentrations (2.26, 4.52, 6.78, and 9.05 M) on the induction and subsequent regeneration of embryogenic calli. On the second experiment, the pre-regeneration phase was modified; calli were subjected to darkness or diffuse light conditions for one, two, and three weeks. In embryogenesis induction, 35% calligenesis was obtained using the MS culture medium supplemented with 6.78 M of 2,4-D and 2.4 gL-1 Phytagel , whereas on the control treatment (MS medium supplemented with 9.05 M of 2,4-D and 3 gL-1 Phytagel ) 24% calligenesis was obtained. In addition, regeneration percentages were improved (22% and 16% for calli induced with the above treatments, respectively). Furthermore, in light exposure experiments, the best result was obtained by exposing the embryogenic calli to darkness for one week in pre-regeneration, followed by direct light exposure during the regeneration phase. Universidad de Costa Rica 2005-12-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.ucr.ac.cr/index.php/rbt/article/view/14598 10.15517/rbt.v53i3-4.14598 Revista de Biología Tropical; Vol. 53 No. 3-4 (2005): Volume 53 - Regular number 3-4 – September-December 2005; 361–368 Revista de Biología Tropical; Vol. 53 Núm. 3-4 (2005): Volumen 53 - Número regular 3-4 – Setiembre-Diciembre 2005; 361–368 Revista Biología Tropical; Vol. 53 N.º 3-4 (2005): Volume 53 - Regular number 3-4 – September-December 2005; 361–368 2215-2075 0034-7744 10.15517/rbt.v53i3-4 eng https://revistas.ucr.ac.cr/index.php/rbt/article/view/14598/13848 Copyright (c) 2005 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0
institution Universidad de Costa Rica
collection Revista de Biología Tropical
language eng
format Online
author Meneses, Allan
Flores, Dora
Muñoz, Miguel
Arrieta, Griselda
Espinoza, Ana M
spellingShingle Meneses, Allan
Flores, Dora
Muñoz, Miguel
Arrieta, Griselda
Espinoza, Ana M
Effect of 2,4-D, hydric stress and light on indica rice (Oryza sativa) somatic embryogenesis
author_facet Meneses, Allan
Flores, Dora
Muñoz, Miguel
Arrieta, Griselda
Espinoza, Ana M
author_sort Meneses, Allan
description With the purpose of increasing the embryogenesis regeneration process in vitroplants obtained from somatic embryos of the indica rice variety CR-5272 (Oryza sativa L.), two independent experiments were performed. The first experiment consisted in the effect of combination of three concentrations of the gelling agent Phytagel (1.8, 2.4, and 3 gL-1) and four 2,4-D concentrations (2.26, 4.52, 6.78, and 9.05 M) on the induction and subsequent regeneration of embryogenic calli. On the second experiment, the pre-regeneration phase was modified; calli were subjected to darkness or diffuse light conditions for one, two, and three weeks. In embryogenesis induction, 35% calligenesis was obtained using the MS culture medium supplemented with 6.78 M of 2,4-D and 2.4 gL-1 Phytagel , whereas on the control treatment (MS medium supplemented with 9.05 M of 2,4-D and 3 gL-1 Phytagel ) 24% calligenesis was obtained. In addition, regeneration percentages were improved (22% and 16% for calli induced with the above treatments, respectively). Furthermore, in light exposure experiments, the best result was obtained by exposing the embryogenic calli to darkness for one week in pre-regeneration, followed by direct light exposure during the regeneration phase.
title Effect of 2,4-D, hydric stress and light on indica rice (Oryza sativa) somatic embryogenesis
title_short Effect of 2,4-D, hydric stress and light on indica rice (Oryza sativa) somatic embryogenesis
title_full Effect of 2,4-D, hydric stress and light on indica rice (Oryza sativa) somatic embryogenesis
title_fullStr Effect of 2,4-D, hydric stress and light on indica rice (Oryza sativa) somatic embryogenesis
title_full_unstemmed Effect of 2,4-D, hydric stress and light on indica rice (Oryza sativa) somatic embryogenesis
title_sort effect of 2,4-d, hydric stress and light on indica rice (oryza sativa) somatic embryogenesis
title_alt Effect of 2,4-D, hydric stress and light on indica rice (Oryza sativa) somatic embryogenesis
publisher Universidad de Costa Rica
publishDate 2005
url https://revistas.ucr.ac.cr/index.php/rbt/article/view/14598
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