Detección inmunoenzimática de la toxina de la bacteria Clostridium tetani: una alternativa a las pruebas biológicas con ratones

Cell-free extracts from 20 strains of Clostridium tetani isolated from soil samples, were tested for tetanus toxin production using an enzyme immunoassay. All the extracts were classified as positive for the toxin presence, and eight of them showed absorbance values corresponding to tetanus toxin co...

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Detalles Bibliográficos
Autores principales:	Chaves, Fernando, León, Guillermo, Hernández Chavarría, Francisco
Formato:	Online
Idioma:	spa
Publicado:	Universidad de Costa Rica 2006
Acceso en línea:	https://revistas.ucr.ac.cr/index.php/rbt/article/view/14108

id	RBT14108
record_format	ojs
spelling	RBT141082022-05-30T18:41:38Z Immunoenzimatic detection of the Clostridium tetani bacterial toxin: an alternative to mice bioassays Detección inmunoenzimática de la toxina de la bacteria Clostridium tetani: una alternativa a las pruebas biológicas con ratones Chaves, Fernando León, Guillermo Hernández Chavarría, Francisco clostridium tetani tetanus toxin enzyme immunoassay biological assay clostridium tetani tetanus toxin enzyme immunoassay biological assay Cell-free extracts from 20 strains of Clostridium tetani isolated from soil samples, were tested for tetanus toxin production using an enzyme immunoassay. All the extracts were classified as positive for the toxin presence, and eight of them showed absorbance values corresponding to tetanus toxin concentrations between 3.2 and 88 ng/ml; thus, they fell within the linear absorbance range (0.135-0.317). All dilutions of toxin used to obtain the calibration curve (0.0071 to 1.1 ng) were lethal for mice. Cell-free extracts from 20 strains of Clostridium tetani isolated from soil samples, were tested for tetanus toxin production using an enzyme immunoassay. All the extracts were classified as positive for the toxin presence, and eight of them showed absorbance values corresponding to tetanus toxin concentrations between 3.2 and 88 ng/ml; thus, they fell within the linear absorbance range (0.135-0.317). All dilutions of toxin used to obtain the calibration curve (0.0071 to 1.1 ng) were lethal for mice. Universidad de Costa Rica 2006-06-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article application/pdf https://revistas.ucr.ac.cr/index.php/rbt/article/view/14108 10.15517/rbt.v54i2.14108 Revista de Biología Tropical; Vol. 54 No. 2 (2006): Volume 54 - Regular number 2 - June 2006; 253–256 Revista de Biología Tropical; Vol. 54 Núm. 2 (2006): Volumen 54 - Número regular 2 - Junio 2006; 253–256 Revista Biología Tropical; Vol. 54 N.º 2 (2006): Volume 54 - Regular number 2 - June 2006; 253–256 2215-2075 0034-7744 10.15517/rbt.v54i2 spa https://revistas.ucr.ac.cr/index.php/rbt/article/view/14108/13420 Copyright (c) 2006 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0
institution	Universidad de Costa Rica
collection	Revista de Biología Tropical
language	spa
format	Online
author	Chaves, Fernando León, Guillermo Hernández Chavarría, Francisco
spellingShingle	Chaves, Fernando León, Guillermo Hernández Chavarría, Francisco Detección inmunoenzimática de la toxina de la bacteria Clostridium tetani: una alternativa a las pruebas biológicas con ratones
author_facet	Chaves, Fernando León, Guillermo Hernández Chavarría, Francisco
author_sort	Chaves, Fernando
description	Cell-free extracts from 20 strains of Clostridium tetani isolated from soil samples, were tested for tetanus toxin production using an enzyme immunoassay. All the extracts were classified as positive for the toxin presence, and eight of them showed absorbance values corresponding to tetanus toxin concentrations between 3.2 and 88 ng/ml; thus, they fell within the linear absorbance range (0.135-0.317). All dilutions of toxin used to obtain the calibration curve (0.0071 to 1.1 ng) were lethal for mice.
title	Detección inmunoenzimática de la toxina de la bacteria Clostridium tetani: una alternativa a las pruebas biológicas con ratones
title_short	Detección inmunoenzimática de la toxina de la bacteria Clostridium tetani: una alternativa a las pruebas biológicas con ratones
title_full	Detección inmunoenzimática de la toxina de la bacteria Clostridium tetani: una alternativa a las pruebas biológicas con ratones
title_fullStr	Detección inmunoenzimática de la toxina de la bacteria Clostridium tetani: una alternativa a las pruebas biológicas con ratones
title_full_unstemmed	Detección inmunoenzimática de la toxina de la bacteria Clostridium tetani: una alternativa a las pruebas biológicas con ratones
title_sort	detección inmunoenzimática de la toxina de la bacteria clostridium tetani: una alternativa a las pruebas biológicas con ratones
title_alt	Immunoenzimatic detection of the Clostridium tetani bacterial toxin: an alternative to mice bioassays
publisher	Universidad de Costa Rica
publishDate	2006
url	https://revistas.ucr.ac.cr/index.php/rbt/article/view/14108
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Detección inmunoenzimática de la toxina de la bacteria Clostridium tetani: una alternativa a las pruebas biológicas con ratones

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