History, applications, methodological issues and perspectives for the use environmental DNA (eDNA) in marine and freshwater environments

Genetic material (short DNA fragments) left behind by species in nonliving components of the environment (e.g. soil, sediment, or water) is defined as environmental DNA (eDNA). This DNA has been previously described as particulate DNA and has been used to detect and describe microbial communities in...

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Autores principales: Díaz-Ferguson, Edgardo E., Moyer, Gregory R.
Formato: Online
Idioma:eng
Publicado: Universidad de Costa Rica 2014
Acceso en línea:https://revistas.ucr.ac.cr/index.php/rbt/article/view/13231
id RBT13231
record_format ojs
spelling RBT132312022-06-09T17:30:45Z History, applications, methodological issues and perspectives for the use environmental DNA (eDNA) in marine and freshwater environments History, applications, methodological issues and perspectives for the use environmental DNA (eDNA) in marine and freshwater environments Díaz-Ferguson, Edgardo E. Moyer, Gregory R. environmental DNA (eDNA) detection probability occupancy models persistence metabarcode minibarcode environmental DNA (eDNA) detection probability occupancy models persistence metabarcode minibarcode Genetic material (short DNA fragments) left behind by species in nonliving components of the environment (e.g. soil, sediment, or water) is defined as environmental DNA (eDNA). This DNA has been previously described as particulate DNA and has been used to detect and describe microbial communities in marine sediments since the mid-1980’s and phytoplankton communities in the water column since the early-1990’s. More recently, eDNA has been used to monitor invasive or endangered vertebrate and invertebrate species. While there is a steady increase in the applicability of eDNA as a monitoring tool, a variety of eDNA applications are emerging in fields such as forensics, population and community ecology, and taxonomy. This review provides scientist an understanding of the methods underlying eDNA detection as well as applications, key methodological considerations, and emerging areas of interest for its use in ecology and conservation of freshwater and marine environments. Genetic material (short DNA fragments) left behind by species in nonliving components of the environment (e.g. soil, sediment, or water) is defined as environmental DNA (eDNA). This DNA has been previously described as particulate DNA and has been used to detect and describe microbial communities in marine sediments since the mid-1980’s and phytoplankton communities in the water column since the early-1990’s. More recently, eDNA has been used to monitor invasive or endangered vertebrate and invertebrate species. While there is a steady increase in the applicability of eDNA as a monitoring tool, a variety of eDNA applications are emerging in fields such as forensics, population and community ecology, and taxonomy. This review provides scientist an understanding of the methods underlying eDNA detection as well as applications, key methodological considerations, and emerging areas of interest for its use in ecology and conservation of freshwater and marine environments. Universidad de Costa Rica 2014-12-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html https://revistas.ucr.ac.cr/index.php/rbt/article/view/13231 10.15517/rbt.v62i4.13231 Revista de Biología Tropical; Vol. 62 No. 4 (2014): Volume 62 – Regular number 4 – December 2014; 1273–1284 Revista de Biología Tropical; Vol. 62 Núm. 4 (2014): Volumen 62 – Número regular 4 – Diciembre 2014; 1273–1284 Revista Biología Tropical; Vol. 62 N.º 4 (2014): Volume 62 – Regular number 4 – December 2014; 1273–1284 2215-2075 0034-7744 10.15517/rbt.v62i4 eng https://revistas.ucr.ac.cr/index.php/rbt/article/view/13231/15457 https://revistas.ucr.ac.cr/index.php/rbt/article/view/13231/15458 Copyright (c) 2014 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0
institution Universidad de Costa Rica
collection Revista de Biología Tropical
language eng
format Online
author Díaz-Ferguson, Edgardo E.
Moyer, Gregory R.
spellingShingle Díaz-Ferguson, Edgardo E.
Moyer, Gregory R.
History, applications, methodological issues and perspectives for the use environmental DNA (eDNA) in marine and freshwater environments
author_facet Díaz-Ferguson, Edgardo E.
Moyer, Gregory R.
author_sort Díaz-Ferguson, Edgardo E.
description Genetic material (short DNA fragments) left behind by species in nonliving components of the environment (e.g. soil, sediment, or water) is defined as environmental DNA (eDNA). This DNA has been previously described as particulate DNA and has been used to detect and describe microbial communities in marine sediments since the mid-1980’s and phytoplankton communities in the water column since the early-1990’s. More recently, eDNA has been used to monitor invasive or endangered vertebrate and invertebrate species. While there is a steady increase in the applicability of eDNA as a monitoring tool, a variety of eDNA applications are emerging in fields such as forensics, population and community ecology, and taxonomy. This review provides scientist an understanding of the methods underlying eDNA detection as well as applications, key methodological considerations, and emerging areas of interest for its use in ecology and conservation of freshwater and marine environments.
title History, applications, methodological issues and perspectives for the use environmental DNA (eDNA) in marine and freshwater environments
title_short History, applications, methodological issues and perspectives for the use environmental DNA (eDNA) in marine and freshwater environments
title_full History, applications, methodological issues and perspectives for the use environmental DNA (eDNA) in marine and freshwater environments
title_fullStr History, applications, methodological issues and perspectives for the use environmental DNA (eDNA) in marine and freshwater environments
title_full_unstemmed History, applications, methodological issues and perspectives for the use environmental DNA (eDNA) in marine and freshwater environments
title_sort history, applications, methodological issues and perspectives for the use environmental dna (edna) in marine and freshwater environments
title_alt History, applications, methodological issues and perspectives for the use environmental DNA (eDNA) in marine and freshwater environments
publisher Universidad de Costa Rica
publishDate 2014
url https://revistas.ucr.ac.cr/index.php/rbt/article/view/13231
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