Identification of gene fragments related to nitrogen deficiency in Eichhornia crassipes (Pontederiaceae)
Eichhornia crassipes is an aquatic plant native to the Amazon River Basin. It has become a serious weed in freshwater habitats in rivers, lakes and reservoirs both in tropical and warm temperate areas worldwide. Some research has stated that it can be used for water phytoremediation, due to its stro...
| Autores principales: | , , , , , |
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| Formato: | Online |
| Idioma: | eng |
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Universidad de Costa Rica
2014
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| Acceso en línea: | https://revistas.ucr.ac.cr/index.php/rbt/article/view/12892 |
| id |
RBT12892 |
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| record_format |
ojs |
| institution |
Universidad de Costa Rica |
| collection |
Revista de Biología Tropical |
| language |
eng |
| format |
Online |
| author |
Fu, Minghui Jiang, Lihua Li, Yuanmei Yan, Guohua Zheng, Lijun Peng, Jinping |
| spellingShingle |
Fu, Minghui Jiang, Lihua Li, Yuanmei Yan, Guohua Zheng, Lijun Peng, Jinping Identification of gene fragments related to nitrogen deficiency in Eichhornia crassipes (Pontederiaceae) |
| author_facet |
Fu, Minghui Jiang, Lihua Li, Yuanmei Yan, Guohua Zheng, Lijun Peng, Jinping |
| author_sort |
Fu, Minghui |
| description |
Eichhornia crassipes is an aquatic plant native to the Amazon River Basin. It has become a serious weed in freshwater habitats in rivers, lakes and reservoirs both in tropical and warm temperate areas worldwide. Some research has stated that it can be used for water phytoremediation, due to its strong assimilation of nitrogen and phosphorus, and the accumulation of heavy metals, and its growth and spread may play an important role in environmental ecology. In order to explore the molecular mechanism of E. crassipes to responses to nitrogen deficiency, we constructed forward and reversed subtracted cDNA libraries for E. crassipes roots under nitrogen deficient condition using a suppressive subtractive hybridization (SSH) method. The forward subtraction included 2 100 clones, and the reversed included 2 650 clones. One thousand clones were randomly selected from each library for sequencing. About 737 (527 unigenes) clones from the forward library and 757 (483 unigenes) clones from the reversed library were informative. Sequence BlastX analysis showed that there were more transporters and adenosylhomocysteinase-like proteins in E. crassipes cultured in nitrogen deficient medium; while, those cultured in nitrogen replete medium had more proteins such as UBR4-like e3 ubiquitin-protein ligase and fasciclin-like arabinogalactan protein 8-like, as well as more cytoskeletal proteins, including actin and tubulin. Cluster of Orthologous Group (COG) analysis also demonstrated that in the forward library, the most ESTs were involved in coenzyme transportation and metabolism. In the reversed library, cytoskeletal ESTs were the most abundant. Gene Ontology (GO) analysis categories demonstrated that unigenes involved in binding, cellular process and electron carrier were the most differentially expressed unigenes between the forward and reversed libraries. All these results suggest that E. crassipes can respond to different nitrogen status by efficiently regulating and controlling some transporter gene expressions, certain metabolism processes, specific signal transduction pathways and cytoskeletal construction. |
| title |
Identification of gene fragments related to nitrogen deficiency in Eichhornia crassipes (Pontederiaceae) |
| title_short |
Identification of gene fragments related to nitrogen deficiency in Eichhornia crassipes (Pontederiaceae) |
| title_full |
Identification of gene fragments related to nitrogen deficiency in Eichhornia crassipes (Pontederiaceae) |
| title_fullStr |
Identification of gene fragments related to nitrogen deficiency in Eichhornia crassipes (Pontederiaceae) |
| title_full_unstemmed |
Identification of gene fragments related to nitrogen deficiency in Eichhornia crassipes (Pontederiaceae) |
| title_sort |
identification of gene fragments related to nitrogen deficiency in eichhornia crassipes (pontederiaceae) |
| title_alt |
Identification of gene fragments related to nitrogen deficiency in Eichhornia crassipes (Pontederiaceae) |
| publisher |
Universidad de Costa Rica |
| publishDate |
2014 |
| url |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/12892 |
| work_keys_str_mv |
AT fuminghui identificationofgenefragmentsrelatedtonitrogendeficiencyineichhorniacrassipespontederiaceae AT jianglihua identificationofgenefragmentsrelatedtonitrogendeficiencyineichhorniacrassipespontederiaceae AT liyuanmei identificationofgenefragmentsrelatedtonitrogendeficiencyineichhorniacrassipespontederiaceae AT yanguohua identificationofgenefragmentsrelatedtonitrogendeficiencyineichhorniacrassipespontederiaceae AT zhenglijun identificationofgenefragmentsrelatedtonitrogendeficiencyineichhorniacrassipespontederiaceae AT pengjinping identificationofgenefragmentsrelatedtonitrogendeficiencyineichhorniacrassipespontederiaceae |
| _version_ |
1810114634271686656 |
| spelling |
RBT128922022-06-09T17:30:45Z Identification of gene fragments related to nitrogen deficiency in Eichhornia crassipes (Pontederiaceae) Identification of gene fragments related to nitrogen deficiency in Eichhornia crassipes (Pontederiaceae) Fu, Minghui Jiang, Lihua Li, Yuanmei Yan, Guohua Zheng, Lijun Peng, Jinping Eichhornia crassipes SSH deficient nitrogen BlastX analysis COG analysis GO analysis Eichhornia crassipes SSH deficient nitrogen BlastX analysis COG analysis GO analysis Eichhornia crassipes is an aquatic plant native to the Amazon River Basin. It has become a serious weed in freshwater habitats in rivers, lakes and reservoirs both in tropical and warm temperate areas worldwide. Some research has stated that it can be used for water phytoremediation, due to its strong assimilation of nitrogen and phosphorus, and the accumulation of heavy metals, and its growth and spread may play an important role in environmental ecology. In order to explore the molecular mechanism of E. crassipes to responses to nitrogen deficiency, we constructed forward and reversed subtracted cDNA libraries for E. crassipes roots under nitrogen deficient condition using a suppressive subtractive hybridization (SSH) method. The forward subtraction included 2 100 clones, and the reversed included 2 650 clones. One thousand clones were randomly selected from each library for sequencing. About 737 (527 unigenes) clones from the forward library and 757 (483 unigenes) clones from the reversed library were informative. Sequence BlastX analysis showed that there were more transporters and adenosylhomocysteinase-like proteins in E. crassipes cultured in nitrogen deficient medium; while, those cultured in nitrogen replete medium had more proteins such as UBR4-like e3 ubiquitin-protein ligase and fasciclin-like arabinogalactan protein 8-like, as well as more cytoskeletal proteins, including actin and tubulin. Cluster of Orthologous Group (COG) analysis also demonstrated that in the forward library, the most ESTs were involved in coenzyme transportation and metabolism. In the reversed library, cytoskeletal ESTs were the most abundant. Gene Ontology (GO) analysis categories demonstrated that unigenes involved in binding, cellular process and electron carrier were the most differentially expressed unigenes between the forward and reversed libraries. All these results suggest that E. crassipes can respond to different nitrogen status by efficiently regulating and controlling some transporter gene expressions, certain metabolism processes, specific signal transduction pathways and cytoskeletal construction. Eichhornia crassipes is an aquatic plant native to the Amazon River Basin. It has become a serious weed in freshwater habitats in rivers, lakes and reservoirs both in tropical and warm temperate areas worldwide. Some research has stated that it can be used for water phytoremediation, due to its strong assimilation of nitrogen and phosphorus, and the accumulation of heavy metals, and its growth and spread may play an important role in environmental ecology. In order to explore the molecular mechanism of E. crassipes to responses to nitrogen deficiency, we constructed forward and reversed subtracted cDNA libraries for E. crassipes roots under nitrogen deficient condition using a suppressive subtractive hybridization (SSH) method. The forward subtraction included 2 100 clones, and the reversed included 2 650 clones. One thousand clones were randomly selected from each library for sequencing. About 737 (527 unigenes) clones from the forward library and 757 (483 unigenes) clones from the reversed library were informative. Sequence BlastX analysis showed that there were more transporters and adenosylhomocysteinase-like proteins in E. crassipes cultured in nitrogen deficient medium; while, those cultured in nitrogen replete medium had more proteins such as UBR4-like e3 ubiquitin-protein ligase and fasciclin-like arabinogalactan protein 8-like, as well as more cytoskeletal proteins, including actin and tubulin. Cluster of Orthologous Group (COG) analysis also demonstrated that in the forward library, the most ESTs were involved in coenzyme transportation and metabolism. In the reversed library, cytoskeletal ESTs were the most abundant. Gene Ontology (GO) analysis categories demonstrated that unigenes involved in binding, cellular process and electron carrier were the most differentially expressed unigenes between the forward and reversed libraries. All these results suggest that E. crassipes can respond to different nitrogen status by efficiently regulating and controlling some transporter gene expressions, certain metabolism processes, specific signal transduction pathways and cytoskeletal construction. Universidad de Costa Rica 2014-12-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Contribution application/pdf text/html https://revistas.ucr.ac.cr/index.php/rbt/article/view/12892 10.15517/rbt.v62i4.12892 Revista de Biología Tropical; Vol. 62 No. 4 (2014): Volume 62 – Regular number 4 – December 2014; 1637–1648 Revista de Biología Tropical; Vol. 62 Núm. 4 (2014): Volumen 62 – Número regular 4 – Diciembre 2014; 1637–1648 Revista Biología Tropical; Vol. 62 N.º 4 (2014): Volume 62 – Regular number 4 – December 2014; 1637–1648 2215-2075 0034-7744 10.15517/rbt.v62i4 eng https://revistas.ucr.ac.cr/index.php/rbt/article/view/12892/15526 https://revistas.ucr.ac.cr/index.php/rbt/article/view/12892/15527 Copyright (c) 2014 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0 |