Presence of Streptococcus Mutans Genotype C in Peruvian Children and Adolescents with Caries
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| Formato: | Online |
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Universidad de Costa Rica
2018
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| Acceso en línea: | https://revistas.ucr.ac.cr/index.php/Odontos/article/view/34279 |
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ODONTOS34279 |
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ODONTOS342792021-06-09T22:07:57Z Presence of Streptococcus Mutans Genotype C in Peruvian Children and Adolescents with Caries Delgadillo Avila DDS, MSc, PhD, Juana R. Espinoza Escajadillo Blgo, MSc, Sofía B. Campodónico Reátegui DDS, MSc, Carlos H. Evaristo Chiyong DDS, MSc, Teresa A. Cáceres de Barces DDS, MSc, Lita Gómez Meza DDS, MSc, Dora N. Chacón Uscamaita, Pamela R. Streptococcus mutans Genetics Saliva Caries Caries is one of the diseases of infectious nature, chronic transmissible very prevalent in Peru, related to the presence of Streptococcus mutans, hygienic and nutritional habits. Objective: The purpose of this study was to determine the presence of genotype C in Streptococcus mutans in Peruvian children and adolescents, using the PCR-Multiplex technique; and its association with the prevalence of dental caries. Materials and methods: The study was done with a sample of 78 children and adolescents of both sexes from Lima. The study consisted of two phases, on the first one the stimulated saliva was obtained, for the bacterial culture, the same ones that were grown on Mitis Salivarius agar with bacitracin and sulfisoxasol. On the second phase, genotyping was carried out according to its enzymatic profile. For the extraction of DNA, the Gene Extraction Kit GG-1 Bacterial DNA was used, for which cultures of Streptococcus sp strains were performed in the BHI broth with sucrose at 37ºC for 24 hours. Results: The presence of Streptococcus mutans was evidenced in 59 (75.6%) of 78 saliva samples. The results of the genotyping by PCR Multiplex demonstrate the presence of 22 saliva samples of Streptococcus mutans with genotypes C (37,297%) and 37 samples 62, 71 % without this Genotype. Conclusions: The results showed that the presence of genotype C is not related to sex, age group or the presence of dental caries. Universidad de Costa Rica 2018-08-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article Original Clinical Research Articles application/pdf text/html https://revistas.ucr.ac.cr/index.php/Odontos/article/view/34279 10.15517/ijds.2018.34279 Odovtos - International Journal of Dental Sciences; Vol. 20 No. 3 (2018): Odovtos - International Journal of Dental Sciences; 121-129 Odovtos International Journal of Dental Sciences; Vol. 20 Núm. 3 (2018): Odovtos - International Journal of Dental Sciences; 121-129 2215-3411 1659-1046 10.15517/ijds.v20i3 eng https://revistas.ucr.ac.cr/index.php/Odontos/article/view/34279/33788 https://revistas.ucr.ac.cr/index.php/Odontos/article/view/34279/33938 Copyright (c) 2018 Odovtos - International Journal of Dental Sciences |
| institution |
Universidad de Costa Rica |
| collection |
Odovtos International Journal of Dental Sciences |
| language |
eng |
| format |
Online |
| author |
Delgadillo Avila DDS, MSc, PhD, Juana R. Espinoza Escajadillo Blgo, MSc, Sofía B. Campodónico Reátegui DDS, MSc, Carlos H. Evaristo Chiyong DDS, MSc, Teresa A. Cáceres de Barces DDS, MSc, Lita Gómez Meza DDS, MSc, Dora N. Chacón Uscamaita, Pamela R. |
| spellingShingle |
Delgadillo Avila DDS, MSc, PhD, Juana R. Espinoza Escajadillo Blgo, MSc, Sofía B. Campodónico Reátegui DDS, MSc, Carlos H. Evaristo Chiyong DDS, MSc, Teresa A. Cáceres de Barces DDS, MSc, Lita Gómez Meza DDS, MSc, Dora N. Chacón Uscamaita, Pamela R. Presence of Streptococcus Mutans Genotype C in Peruvian Children and Adolescents with Caries |
| author_facet |
Delgadillo Avila DDS, MSc, PhD, Juana R. Espinoza Escajadillo Blgo, MSc, Sofía B. Campodónico Reátegui DDS, MSc, Carlos H. Evaristo Chiyong DDS, MSc, Teresa A. Cáceres de Barces DDS, MSc, Lita Gómez Meza DDS, MSc, Dora N. Chacón Uscamaita, Pamela R. |
| author_sort |
Delgadillo Avila DDS, MSc, PhD, Juana R. |
| title |
Presence of Streptococcus Mutans Genotype C in Peruvian Children and Adolescents with Caries |
| title_short |
Presence of Streptococcus Mutans Genotype C in Peruvian Children and Adolescents with Caries |
| title_full |
Presence of Streptococcus Mutans Genotype C in Peruvian Children and Adolescents with Caries |
| title_fullStr |
Presence of Streptococcus Mutans Genotype C in Peruvian Children and Adolescents with Caries |
| title_full_unstemmed |
Presence of Streptococcus Mutans Genotype C in Peruvian Children and Adolescents with Caries |
| title_sort |
presence of streptococcus mutans genotype c in peruvian children and adolescents with caries |
| publisher |
Universidad de Costa Rica |
| publishDate |
2018 |
| url |
https://revistas.ucr.ac.cr/index.php/Odontos/article/view/34279 |
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