Antibacterial Efficacy of a Dispersion of Silver Nanoparticles in Citrate Medium for the Treatment of E. faecalis: an In Vitro Study
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| Formato: | Online |
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Universidad de Costa Rica
2016
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| Acceso en línea: | https://revistas.ucr.ac.cr/index.php/Odontos/article/view/23929 |
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ODONTOS23929 |
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ODONTOS239292021-06-09T00:17:37Z Antibacterial Efficacy of a Dispersion of Silver Nanoparticles in Citrate Medium for the Treatment of E. faecalis: an In Vitro Study Rodríguez DDS, Silvia Ramírez DDS, MSc, Tatiana Montero DDS, MSc, Mauricio Chavarría DDS, MSc, PhD, Daniel Valle PhD, Grettel Rojas MSc, Norman Antimicrobial efficacy Silver nanoparticles Sodium hypochlorite E. faecalis The purpose of this study was to measure the antibacterial efficacy of a dispersion of silver nanoparticles (AgNP) in a citrate medium tested in two E. faecalis strains. AgNP were synthesized, and AgNP citrate medium (AgNP-CM) dispersion was prepared at a concentration of 100 μg/mL. The antibacterial efficacy of AgNP-CM dispersion was evaluated over two E. faecalis strains: ATCC29212 and a wild strain collected from human necrotic teeth. 5% sodium hypochlorite (NaOCl) and sterile saline solution were used as positive and negative controls. 5 and 30-minute contact tests were conducted and each experimental group were replicated 10 times. After 24 hours of incubation, the Log CFU/mL were calculated. The AgNP obtained showed spherical shapes and had 30-60nm size. 5% NaOCl was able to completely eliminate both E. faecalis strains in all groups, showing a significant statistical difference when compared to AgNP-CM dispersion and negative control groups. AgNP-CM dispersion showed a statistically significant decrease in Log CFU/mL averages (p=0,0006) when compared to the sterile saline solution for the ATCC29212 strain during the 30-minute time. Between the 5-minute and 30-minute groups, a significant bacterial count decrease was also observed (p= 0,0128). The antibacterial efficacy of the dispersion was greater for the ATCC29212 strain than the wild strain, were the effect diminished. AgNP-CM dispersion showed a significantly lower antibacterial efficacy against E. faecalis than the 5% NaOCl at the tested times. Universidad de Costa Rica 2016-06-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article Laboratory research application/pdf text/html https://revistas.ucr.ac.cr/index.php/Odontos/article/view/23929 10.15517/ijds.v18i2.23929 Odovtos - International Journal of Dental Sciences; Vol. 18 No. 2 (2016): Odovtos-Int. J. Dent. Sc. v18,2 (MAY-AUGUST) -2016; 99-107 Odovtos International Journal of Dental Sciences; Vol. 18 Núm. 2 (2016): Odovtos-Int. J. Dent. Sc. v18,2 (MAY-AUGUST) -2016; 99-107 2215-3411 1659-1046 10.15517/ijds.v18i2 eng https://revistas.ucr.ac.cr/index.php/Odontos/article/view/23929/24091 https://revistas.ucr.ac.cr/index.php/Odontos/article/view/23929/24431 Copyright (c) 2016 Odovtos - International Journal of Dental Sciences |
| institution |
Universidad de Costa Rica |
| collection |
Odovtos International Journal of Dental Sciences |
| language |
eng |
| format |
Online |
| author |
Rodríguez DDS, Silvia Ramírez DDS, MSc, Tatiana Montero DDS, MSc, Mauricio Chavarría DDS, MSc, PhD, Daniel Valle PhD, Grettel Rojas MSc, Norman |
| spellingShingle |
Rodríguez DDS, Silvia Ramírez DDS, MSc, Tatiana Montero DDS, MSc, Mauricio Chavarría DDS, MSc, PhD, Daniel Valle PhD, Grettel Rojas MSc, Norman Antibacterial Efficacy of a Dispersion of Silver Nanoparticles in Citrate Medium for the Treatment of E. faecalis: an In Vitro Study |
| author_facet |
Rodríguez DDS, Silvia Ramírez DDS, MSc, Tatiana Montero DDS, MSc, Mauricio Chavarría DDS, MSc, PhD, Daniel Valle PhD, Grettel Rojas MSc, Norman |
| author_sort |
Rodríguez DDS, Silvia |
| title |
Antibacterial Efficacy of a Dispersion of Silver Nanoparticles in Citrate Medium for the Treatment of E. faecalis: an In Vitro Study |
| title_short |
Antibacterial Efficacy of a Dispersion of Silver Nanoparticles in Citrate Medium for the Treatment of E. faecalis: an In Vitro Study |
| title_full |
Antibacterial Efficacy of a Dispersion of Silver Nanoparticles in Citrate Medium for the Treatment of E. faecalis: an In Vitro Study |
| title_fullStr |
Antibacterial Efficacy of a Dispersion of Silver Nanoparticles in Citrate Medium for the Treatment of E. faecalis: an In Vitro Study |
| title_full_unstemmed |
Antibacterial Efficacy of a Dispersion of Silver Nanoparticles in Citrate Medium for the Treatment of E. faecalis: an In Vitro Study |
| title_sort |
antibacterial efficacy of a dispersion of silver nanoparticles in citrate medium for the treatment of e. faecalis: an in vitro study |
| publisher |
Universidad de Costa Rica |
| publishDate |
2016 |
| url |
https://revistas.ucr.ac.cr/index.php/Odontos/article/view/23929 |
| work_keys_str_mv |
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