Capsule development, in vitro germination and plantlet acclimatization in Phragmipedium humboldtii, P. longifolium and P. pearcei

Capsule development, in vitro germination and plantlet acclimatization in Phragmipedium humboldtii, P. longifolium and P. pearcei

Capsule development from pollination to full ripeness was evaluated in Phragmipedium longifolium, P. pearcei and P. humboldtii. Besides, seed viability, analyzed in each capsule by means of the tetrazolium chloride staining, was determined. Considering seed viability, germination rate was corrected...

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Autores principales: Muñoz, Melania, Jiménez, Víctor M.
Formato: Online
Idioma:eng
Publicado: Universidad de Costa Rica 2008
Acceso en línea:https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/7924
id LANKESTERIANA7924
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spelling LANKESTERIANA79242021-06-09T21:10:11Z Capsule development, in vitro germination and plantlet acclimatization in Phragmipedium humboldtii, P. longifolium and P. pearcei Capsule development, in vitro germination and plantlet acclimatization in Phragmipedium humboldtii, P. longifolium and P. pearcei Muñoz, Melania Jiménez, Víctor M. Desarrollo de cápsulas germinación in vitro polinización Phragmipedium orquídeas terrestres cloruro de tetrazolio orquídeas tropicales Capsule development in vitro seed germination pollination terrestrial orchids tetrazolium chloride tropical orchids Capsule development from pollination to full ripeness was evaluated in Phragmipedium longifolium, P. pearcei and P. humboldtii. Besides, seed viability, analyzed in each capsule by means of the tetrazolium chloride staining, was determined. Considering seed viability, germination rate was corrected and expressed as the rate of viable seeds that germinated in the presence and absence of light, on Knudson C and on half-strength Murashige and Skoog culture media. Capsule length remained constant during the evaluation period, while the diameter increased during the first 6-8 weeks and then stagnated. Capsule opening occurred 16 weeks after pollination in P. longifolium, after 9.8 weeks in P. pearcei and after 32 weeks in P. humboldtii. Seed viability averaged 44.7% in P. longifolium, 82.3% in P. pearcei and 34.3% in P. humboldtii. No significant effect of light conditions was evident in any of the species. However, a higher proportion of seeds of P. longifolium and P. pearcei germinated earlier on half-strength Murashige and Skoog medium than on Knudson C. Only 2.9% of the viable seeds of P. humboldtii germinated, while approximately 40% germination occurred in the other two species. Initial growth of the embryos was better in the dark on Knudson C medium, compared to the other treatments studied. Further growth of the seedlings took place under light conditions. Developed plants formed roots and were successfully acclimatized in the greenhouse. Capsule development from pollination to full ripeness was evaluated in Phragmipedium longifolium, P. pearcei and P. humboldtii. Besides, seed viability, analyzed in each capsule by means of the tetrazolium chloride staining, was determined. Considering seed viability, germination rate was corrected and expressed as the rate of viable seeds that germinated in the presence and absence of light, on Knudson C and on half-strength Murashige and Skoog culture media. Capsule length remained constant during the evaluation period, while the diameter increased during the first 6-8 weeks and then stagnated. Capsule opening occurred 16 weeks after pollination in P. longifolium, after 9.8 weeks in P. pearcei and after 32 weeks in P. humboldtii. Seed viability averaged 44.7% in P. longifolium, 82.3% in P. pearcei and 34.3% in P. humboldtii. No significant effect of light conditions was evident in any of the species. However, a higher proportion of seeds of P. longifolium and P. pearcei germinated earlier on half-strength Murashige and Skoog medium than on Knudson C. Only 2.9% of the viable seeds of P. humboldtii germinated, while approximately 40% germination occurred in the other two species. Initial growth of the embryos was better in the dark on Knudson C medium, compared to the other treatments studied. Further growth of the seedlings took place under light conditions. Developed plants formed roots and were successfully acclimatized in the greenhouse. Universidad de Costa Rica 2008-08-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Text Article application/pdf https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/7924 10.15517/lank.v0i0.7924 Lankesteriana: International Journal on Orchidology; 2008: Lankesteriana : Volumen 8, Número 2 Lankesteriana: International Journal on Orchidology; 2008: Lankesteriana : Volumen 8, Número 2 2215-2067 1409-3871 eng https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/7924/7558
institution Universidad de Costa Rica
collection Lankesteriana: International Journal on Orchidology
language eng
format Online
author Muñoz, Melania
Jiménez, Víctor M.
spellingShingle Muñoz, Melania
Jiménez, Víctor M.
Capsule development, in vitro germination and plantlet acclimatization in Phragmipedium humboldtii, P. longifolium and P. pearcei
author_facet Muñoz, Melania
Jiménez, Víctor M.
author_sort Muñoz, Melania
description Capsule development from pollination to full ripeness was evaluated in Phragmipedium longifolium, P. pearcei and P. humboldtii. Besides, seed viability, analyzed in each capsule by means of the tetrazolium chloride staining, was determined. Considering seed viability, germination rate was corrected and expressed as the rate of viable seeds that germinated in the presence and absence of light, on Knudson C and on half-strength Murashige and Skoog culture media. Capsule length remained constant during the evaluation period, while the diameter increased during the first 6-8 weeks and then stagnated. Capsule opening occurred 16 weeks after pollination in P. longifolium, after 9.8 weeks in P. pearcei and after 32 weeks in P. humboldtii. Seed viability averaged 44.7% in P. longifolium, 82.3% in P. pearcei and 34.3% in P. humboldtii. No significant effect of light conditions was evident in any of the species. However, a higher proportion of seeds of P. longifolium and P. pearcei germinated earlier on half-strength Murashige and Skoog medium than on Knudson C. Only 2.9% of the viable seeds of P. humboldtii germinated, while approximately 40% germination occurred in the other two species. Initial growth of the embryos was better in the dark on Knudson C medium, compared to the other treatments studied. Further growth of the seedlings took place under light conditions. Developed plants formed roots and were successfully acclimatized in the greenhouse.
title Capsule development, in vitro germination and plantlet acclimatization in Phragmipedium humboldtii, P. longifolium and P. pearcei
title_short Capsule development, in vitro germination and plantlet acclimatization in Phragmipedium humboldtii, P. longifolium and P. pearcei
title_full Capsule development, in vitro germination and plantlet acclimatization in Phragmipedium humboldtii, P. longifolium and P. pearcei
title_fullStr Capsule development, in vitro germination and plantlet acclimatization in Phragmipedium humboldtii, P. longifolium and P. pearcei
title_full_unstemmed Capsule development, in vitro germination and plantlet acclimatization in Phragmipedium humboldtii, P. longifolium and P. pearcei
title_sort capsule development, in vitro germination and plantlet acclimatization in phragmipedium humboldtii, p. longifolium and p. pearcei
title_alt Capsule development, in vitro germination and plantlet acclimatization in Phragmipedium humboldtii, P. longifolium and P. pearcei
publisher Universidad de Costa Rica
publishDate 2008
url https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/7924
work_keys_str_mv AT munozmelania capsuledevelopmentinvitrogerminationandplantletacclimatizationinphragmipediumhumboldtiiplongifoliumandppearcei
AT jimenezvictorm capsuledevelopmentinvitrogerminationandplantletacclimatizationinphragmipediumhumboldtiiplongifoliumandppearcei
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