Comparative floral surface micromorphology helps to discriminate between species of Paphiopedilum (Orchidaceae: Cypripedioideae) from Peninsular Malaysia
Abstract. The floral micromorphology of critically endangered Paphiopedilum Pfitzer [P. barbatum (Lindl.) Pfitzer, P. callosum var. sublaeve (Rchb.f.) P.J.Cribb and P. niveum (Rchb.f.) Stein] were analyzed concerning either infrageneric taxonomy or physioecological demands. The fir...
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| Formato: | Online |
| Idioma: | eng |
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Universidad de Costa Rica
2021
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| Acceso en línea: | https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/46714 |
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LANKESTERIANA46714 |
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| record_format |
ojs |
| institution |
Universidad de Costa Rica |
| collection |
Lankesteriana: International Journal on Orchidology |
| language |
eng |
| format |
Online |
| author |
Besi, Edward E. Jia, Lam S. Mustafa, Muskhazli Yong, Christina S. Y. Go, Rusea |
| spellingShingle |
Besi, Edward E. Jia, Lam S. Mustafa, Muskhazli Yong, Christina S. Y. Go, Rusea Comparative floral surface micromorphology helps to discriminate between species of Paphiopedilum (Orchidaceae: Cypripedioideae) from Peninsular Malaysia |
| author_facet |
Besi, Edward E. Jia, Lam S. Mustafa, Muskhazli Yong, Christina S. Y. Go, Rusea |
| author_sort |
Besi, Edward E. |
| description |
Abstract. The floral micromorphology of critically endangered Paphiopedilum Pfitzer [P. barbatum (Lindl.) Pfitzer, P. callosum var. sublaeve (Rchb.f.) P.J.Cribb and P. niveum (Rchb.f.) Stein] were analyzed concerning either infrageneric taxonomy or physioecological demands. The first two species are phylogenetically close and superficially identical but occur with distinct phytogeographical distributions in the region. The third species is a phylogenetically distant congener that inhabits limestone areas in the northern part of Peninsular Malaysia. Using scanning electron microscopy (SEM), we investigated the surface of the dorsal sepal, synsepal, lateral petals, pouch or labellum, and staminode. Amongst the investigated features were epicuticular waxes, epicuticular ornamentation, trichome distribution and type, pustular glands, and papillae. Our study supports the distinction of P. barbatum from P. callosum var. sublaeve, which belong to subgenus Paphiopedilum, and from P. niveum, a species belonging to subgenus Brachypetalum, a separated monophyletic clade. Comparatively, P. barbatum has Type III non-glandular trichomes on the margin of its lateral petals, which are absent in P. callosum var. sublaeve. Paphiopedilum callosum var. sublaeve and P. niveum are distinguishable from P. barbatum by a confined distribution of papillae. The epicuticular ornamentation and distribution of trichomes on staminode discriminate between P. barbatum and P. callosum var. sublaeve and differentiates them from P. niveum. Compared to P. barbatum and P. niveum, stomata in P. callosum var. sublaeve were superficial with prominently raised guard cells. From the physioecological view, the absence of glandular trichomes, and the low occurrence of papillae and stomata on the floral parts explain the unscented flowers of P. barbatum and P. callosum var. sublaeve. A combination of the features examined is taxonomically valuable for delimitation of the species at the infrageneric level, although the diagnostic characters are far inadequate for a generic taxonomic revision. A study with a more extensive sampling from the three subgenera of Paphiopedilum, including subgenus Parvisepalum, is anticipated to elucidate the level of variation of the analyzed microcharacters.
Key Words: physioecological importance, Peninsular Malaysia, subgenus Brachypetalum, subgenus Paphiopedilum, scanning electron microscopy, taxonomic delimitation |
| title |
Comparative floral surface micromorphology helps to discriminate between species of Paphiopedilum (Orchidaceae: Cypripedioideae) from Peninsular Malaysia |
| title_short |
Comparative floral surface micromorphology helps to discriminate between species of Paphiopedilum (Orchidaceae: Cypripedioideae) from Peninsular Malaysia |
| title_full |
Comparative floral surface micromorphology helps to discriminate between species of Paphiopedilum (Orchidaceae: Cypripedioideae) from Peninsular Malaysia |
| title_fullStr |
Comparative floral surface micromorphology helps to discriminate between species of Paphiopedilum (Orchidaceae: Cypripedioideae) from Peninsular Malaysia |
| title_full_unstemmed |
Comparative floral surface micromorphology helps to discriminate between species of Paphiopedilum (Orchidaceae: Cypripedioideae) from Peninsular Malaysia |
| title_sort |
comparative floral surface micromorphology helps to discriminate between species of paphiopedilum (orchidaceae: cypripedioideae) from peninsular malaysia |
| title_alt |
Comparative floral surface micromorphology helps to discriminate between species of Paphiopedilum (Orchidaceae: Cypripedioideae) from Peninsular Malaysia |
| publisher |
Universidad de Costa Rica |
| publishDate |
2021 |
| url |
https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/46714 |
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LANKESTERIANA467142022-08-26T18:01:31Z Comparative floral surface micromorphology helps to discriminate between species of Paphiopedilum (Orchidaceae: Cypripedioideae) from Peninsular Malaysia Comparative floral surface micromorphology helps to discriminate between species of Paphiopedilum (Orchidaceae: Cypripedioideae) from Peninsular Malaysia Besi, Edward E. Jia, Lam S. Mustafa, Muskhazli Yong, Christina S. Y. Go, Rusea Abstract. The floral micromorphology of critically endangered Paphiopedilum Pfitzer [P. barbatum (Lindl.) Pfitzer, P. callosum var. sublaeve (Rchb.f.) P.J.Cribb and P. niveum (Rchb.f.) Stein] were analyzed concerning either infrageneric taxonomy or physioecological demands. The first two species are phylogenetically close and superficially identical but occur with distinct phytogeographical distributions in the region. The third species is a phylogenetically distant congener that inhabits limestone areas in the northern part of Peninsular Malaysia. Using scanning electron microscopy (SEM), we investigated the surface of the dorsal sepal, synsepal, lateral petals, pouch or labellum, and staminode. Amongst the investigated features were epicuticular waxes, epicuticular ornamentation, trichome distribution and type, pustular glands, and papillae. Our study supports the distinction of P. barbatum from P. callosum var. sublaeve, which belong to subgenus Paphiopedilum, and from P. niveum, a species belonging to subgenus Brachypetalum, a separated monophyletic clade. Comparatively, P. barbatum has Type III non-glandular trichomes on the margin of its lateral petals, which are absent in P. callosum var. sublaeve. Paphiopedilum callosum var. sublaeve and P. niveum are distinguishable from P. barbatum by a confined distribution of papillae. The epicuticular ornamentation and distribution of trichomes on staminode discriminate between P. barbatum and P. callosum var. sublaeve and differentiates them from P. niveum. Compared to P. barbatum and P. niveum, stomata in P. callosum var. sublaeve were superficial with prominently raised guard cells. From the physioecological view, the absence of glandular trichomes, and the low occurrence of papillae and stomata on the floral parts explain the unscented flowers of P. barbatum and P. callosum var. sublaeve. A combination of the features examined is taxonomically valuable for delimitation of the species at the infrageneric level, although the diagnostic characters are far inadequate for a generic taxonomic revision. A study with a more extensive sampling from the three subgenera of Paphiopedilum, including subgenus Parvisepalum, is anticipated to elucidate the level of variation of the analyzed microcharacters. Key Words: physioecological importance, Peninsular Malaysia, subgenus Brachypetalum, subgenus Paphiopedilum, scanning electron microscopy, taxonomic delimitation Abstract. The floral micromorphology of critically endangered Paphiopedilum Pfitzer [P. barbatum (Lindl.) Pfitzer, P. callosum var. sublaeve (Rchb.f.) P.J.Cribb and P. niveum (Rchb.f.) Stein] were analyzed concerning either infrageneric taxonomy or physioecological demands. The first two species are phylogenetically close and superficially identical but occur with distinct phytogeographical distributions in the region. The third species is a phylogenetically distant congener that inhabits limestone areas in the northern part of Peninsular Malaysia. Using scanning electron microscopy (SEM), we investigated the surface of the dorsal sepal, synsepal, lateral petals, pouch or labellum, and staminode. Amongst the investigated features were epicuticular waxes, epicuticular ornamentation, trichome distribution and type, pustular glands, and papillae. Our study supports the distinction of P. barbatum from P. callosum var. sublaeve, which belong to subgenus Paphiopedilum, and from P. niveum, a species belonging to subgenus Brachypetalum, a separated monophyletic clade. Comparatively, P. barbatum has Type III non-glandular trichomes on the margin of its lateral petals, which are absent in P. callosum var. sublaeve. Paphiopedilum callosum var. sublaeve and P. niveum are distinguishable from P. barbatum by a confined distribution of papillae. The epicuticular ornamentation and distribution of trichomes on staminode discriminate between P. barbatum and P. callosum var. sublaeve and differentiates them from P. niveum. Compared to P. barbatum and P. niveum, stomata in P. callosum var. sublaeve were superficial with prominently raised guard cells. From the physioecological view, the absence of glandular trichomes, and the low occurrence of papillae and stomata on the floral parts explain the unscented flowers of P. barbatum and P. callosum var. sublaeve. A combination of the features examined is taxonomically valuable for delimitation of the species at the infrageneric level, although the diagnostic characters are far inadequate for a generic taxonomic revision. A study with a more extensive sampling from the three subgenera of Paphiopedilum, including subgenus Parvisepalum, is anticipated to elucidate the level of variation of the analyzed microcharacters. Key Words: physioecological importance, Peninsular Malaysia, subgenus Brachypetalum, subgenus Paphiopedilum, scanning electron microscopy, taxonomic delimitation Universidad de Costa Rica 2021-04-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article application/pdf application/epub+zip https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/46714 10.15517/lank.v21i1.46714 Lankesteriana: International Journal on Orchidology; 2021: Lankesteriana: Volume 21, Number 1 (January–April); 17–31 Lankesteriana: International Journal on Orchidology; 2021: Lankesteriana: Volumen 21, Número 1 (Enero–Abril); 17–31 2215-2067 1409-3871 eng https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/46714/46283 https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/46714/49807 Copyright (c) 2021 Lankester Botanical Garden, University of Costa Rica |