The effect of smoke derivatives and carbon utilisation on symbiotic germination of the endangered Pterostylis despectans
Orchids are highly dependent on exogenous nutritional sources and mycorrhizal associations to survive, particularly when challenged by extreme environmental stress such as bushfires that contribute significantly to its existence in nature. In this study, the effect of smoke derivatives and carbon ut...
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| Formato: | Online |
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Universidad de Costa Rica
2018
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| Acceso en línea: | https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/34534 |
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LANKESTERIANA345342022-09-29T23:20:26Z The effect of smoke derivatives and carbon utilisation on symbiotic germination of the endangered Pterostylis despectans The effect of smoke derivatives and carbon utilisation on symbiotic germination of the endangered Pterostylis despectans Ritmejeryté, Edita Obvintseva, Anna Huynh, Tien Orchids are highly dependent on exogenous nutritional sources and mycorrhizal associations to survive, particularly when challenged by extreme environmental stress such as bushfires that contribute significantly to its existence in nature. In this study, the effect of smoke derivatives and carbon utilisation was explored to improve germination and seedling establishment of an Australian endangered orchid, Pterostylis despectans (Lowly Greenhood) and on mycorrhizal fungi to improve its survivorship in the rhizosphere and functionality on host plants. Stored seeds were germinated in vitro with peloton-isolated fungal isolates with varying concentrations of smoke water (0–1.0 mL L-1) to simulate fire and sucrose as the carbon source (10 g L-1). Smoke water significantly increased germination, with advanced protocorms and robust seedlings produced. Sucrose inhibited germination such that protocorms and leafing was absent with sucrose inclusion. Fungal isolates were highly variable on its germination efficacy and tolerance to smoke water, highlighting the importance of fungal diversity and supports research-based conservation strategies to circumvent environmental challenges. Orchids are highly dependent on exogenous nutritional sources and mycorrhizal associations to survive, particularly when challenged by extreme environmental stress such as bushfires that contribute significantly to its existence in nature. In this study, the effect of smoke derivatives and carbon utilisation was explored to improve germination and seedling establishment of an Australian endangered orchid, Pterostylis despectans (Lowly Greenhood) and on mycorrhizal fungi to improve its survivorship in the rhizosphere and functionality on host plants. Stored seeds were germinated in vitro with peloton-isolated fungal isolates with varying concentrations of smoke water (0–1.0 mL L-1) to simulate fire and sucrose as the carbon source (10 g L-1). Smoke water significantly increased germination, with advanced protocorms and robust seedlings produced. Sucrose inhibited germination such that protocorms and leafing was absent with sucrose inclusion. Fungal isolates were highly variable on its germination efficacy and tolerance to smoke water, highlighting the importance of fungal diversity and supports research-based conservation strategies to circumvent environmental challenges. Universidad de Costa Rica 2018-09-13 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article application/pdf https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/34534 10.15517/lank.v18i3.34534 Lankesteriana: International Journal on Orchidology; 2018: Lankesteriana: Volume 18, Number 3 Lankesteriana: International Journal on Orchidology; 2018: Lankesteriana: Volumen 18, Número 3 2215-2067 1409-3871 eng https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/34534/34083 |
| institution |
Universidad de Costa Rica |
| collection |
Lankesteriana: International Journal on Orchidology |
| language |
eng |
| format |
Online |
| author |
Ritmejeryté, Edita Obvintseva, Anna Huynh, Tien |
| spellingShingle |
Ritmejeryté, Edita Obvintseva, Anna Huynh, Tien The effect of smoke derivatives and carbon utilisation on symbiotic germination of the endangered Pterostylis despectans |
| author_facet |
Ritmejeryté, Edita Obvintseva, Anna Huynh, Tien |
| author_sort |
Ritmejeryté, Edita |
| description |
Orchids are highly dependent on exogenous nutritional sources and mycorrhizal associations to survive, particularly when challenged by extreme environmental stress such as bushfires that contribute significantly to its existence in nature. In this study, the effect of smoke derivatives and carbon utilisation was explored to improve germination and seedling establishment of an Australian endangered orchid, Pterostylis despectans (Lowly Greenhood) and on mycorrhizal fungi to improve its survivorship in the rhizosphere and functionality on host plants. Stored seeds were germinated in vitro with peloton-isolated fungal isolates with varying concentrations of smoke water (0–1.0 mL L-1) to simulate fire and sucrose as the carbon source (10 g L-1). Smoke water significantly increased germination, with advanced protocorms and robust seedlings produced. Sucrose inhibited germination such that protocorms and leafing was absent with sucrose inclusion. Fungal isolates were highly variable on its germination efficacy and tolerance to smoke water, highlighting the importance of fungal diversity and supports research-based conservation strategies to circumvent environmental challenges. |
| title |
The effect of smoke derivatives and carbon utilisation on symbiotic germination of the endangered Pterostylis despectans |
| title_short |
The effect of smoke derivatives and carbon utilisation on symbiotic germination of the endangered Pterostylis despectans |
| title_full |
The effect of smoke derivatives and carbon utilisation on symbiotic germination of the endangered Pterostylis despectans |
| title_fullStr |
The effect of smoke derivatives and carbon utilisation on symbiotic germination of the endangered Pterostylis despectans |
| title_full_unstemmed |
The effect of smoke derivatives and carbon utilisation on symbiotic germination of the endangered Pterostylis despectans |
| title_sort |
effect of smoke derivatives and carbon utilisation on symbiotic germination of the endangered pterostylis despectans |
| title_alt |
The effect of smoke derivatives and carbon utilisation on symbiotic germination of the endangered Pterostylis despectans |
| publisher |
Universidad de Costa Rica |
| publishDate |
2018 |
| url |
https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/34534 |
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