Protocorm-like bodies and plant regeneration from foliar explants of Coelogyne flaccida, a horticulturally and medicinally important endangered orchid of Eastern Himalaya

An efficient induction of protocrom-like bodies (PLBs) and plantlet regeneration from the young leaves of in vitro grown seedlings of Coelogyne flaccida, an horticulturally and medicinally important endangered epiphytic orchid, was accomplished in order to develop mass-scale propagation. The young l...

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Autores principales:	De, Kaylan K., Sil, Sujit
Formato:	Online
Idioma:	spa
Publicado:	Universidad de Costa Rica 2015
Acceso en línea:	https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/20747

id	LANKESTERIANA20747
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spelling	LANKESTERIANA207472022-07-20T00:44:32Z Protocorm-like bodies and plant regeneration from foliar explants of Coelogyne flaccida, a horticulturally and medicinally important endangered orchid of Eastern Himalaya Protocorm-like bodies and plant regeneration from foliar explants of Coelogyne flaccida, a horticulturally and medicinally important endangered orchid of Eastern Himalaya De, Kaylan K. Sil, Sujit Coelogyne flaccida foliar explant micropropagation Coelogyne flaccida foliar explant micropropagation An efficient induction of protocrom-like bodies (PLBs) and plantlet regeneration from the young leaves of in vitro grown seedlings of Coelogyne flaccida, an horticulturally and medicinally important endangered epiphytic orchid, was accomplished in order to develop mass-scale propagation. The young leaves (1.5 cm in length) from 110 days old aseptically germinated seedling were grown in vitro in Murashige and Skoog’s (MS) medium supplemented with different concentrations and combinations of NAA ( 0.5-2 mg/L), BAP (0.5-2 mg/L) and Kn ( 0.5-2 mg/L ). The explants produced protocorm-like bodies directly from the epidermal cells of leaf without the formation of intervening callus tissue within four weeks of culture. The highest number of plantlets regenerated through PLBs per explant after 15 weeks was 35-36 in presence of NAA (2mg/l) and Kn (2mg/l). Within 20-25 weeks individual plantlets produced 2-3 leaves and 2-3 roots. Chromosome number from all plants regenerated from leaf explants showed the same chromosome number as the mother plant as 2n = 40. During acclimatization, 80% of the plantlets survived after one month of transplantation. An efficient induction of protocrom-like bodies (PLBs) and plantlet regeneration from the young leaves of in vitro grown seedlings of Coelogyne flaccida, an horticulturally and medicinally important endangered epiphytic orchid, was accomplished in order to develop mass-scale propagation. The young leaves (1.5 cm in length) from 110 days old aseptically germinated seedling were grown in vitro in Murashige and Skoog’s (MS) medium supplemented with different concentrations and combinations of NAA ( 0.5-2 mg/L), BAP (0.5-2 mg/L) and Kn ( 0.5-2 mg/L ). The explants produced protocorm-like bodies directly from the epidermal cells of leaf without the formation of intervening callus tissue within four weeks of culture. The highest number of plantlets regenerated through PLBs per explant after 15 weeks was 35-36 in presence of NAA (2mg/l) and Kn (2mg/l). Within 20-25 weeks individual plantlets produced 2-3 leaves and 2-3 roots. Chromosome number from all plants regenerated from leaf explants showed the same chromosome number as the mother plant as 2n = 40. During acclimatization, 80% of the plantlets survived after one month of transplantation. Universidad de Costa Rica 2015-08-05 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article application/pdf https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/20747 10.15517/lank.v15i2.20747 Lankesteriana: International Journal on Orchidology; 2015: Lankesteriana: Volumen 15, Número 2 Lankesteriana: International Journal on Orchidology; 2015: Lankesteriana: Volumen 15, Número 2 2215-2067 1409-3871 spa https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/20747/20896
institution	Universidad de Costa Rica
collection	Lankesteriana: International Journal on Orchidology
language	spa
format	Online
author	De, Kaylan K. Sil, Sujit
spellingShingle	De, Kaylan K. Sil, Sujit Protocorm-like bodies and plant regeneration from foliar explants of Coelogyne flaccida, a horticulturally and medicinally important endangered orchid of Eastern Himalaya
author_facet	De, Kaylan K. Sil, Sujit
author_sort	De, Kaylan K.
description	An efficient induction of protocrom-like bodies (PLBs) and plantlet regeneration from the young leaves of in vitro grown seedlings of Coelogyne flaccida, an horticulturally and medicinally important endangered epiphytic orchid, was accomplished in order to develop mass-scale propagation. The young leaves (1.5 cm in length) from 110 days old aseptically germinated seedling were grown in vitro in Murashige and Skoog’s (MS) medium supplemented with different concentrations and combinations of NAA ( 0.5-2 mg/L), BAP (0.5-2 mg/L) and Kn ( 0.5-2 mg/L ). The explants produced protocorm-like bodies directly from the epidermal cells of leaf without the formation of intervening callus tissue within four weeks of culture. The highest number of plantlets regenerated through PLBs per explant after 15 weeks was 35-36 in presence of NAA (2mg/l) and Kn (2mg/l). Within 20-25 weeks individual plantlets produced 2-3 leaves and 2-3 roots. Chromosome number from all plants regenerated from leaf explants showed the same chromosome number as the mother plant as 2n = 40. During acclimatization, 80% of the plantlets survived after one month of transplantation.
title	Protocorm-like bodies and plant regeneration from foliar explants of Coelogyne flaccida, a horticulturally and medicinally important endangered orchid of Eastern Himalaya
title_short	Protocorm-like bodies and plant regeneration from foliar explants of Coelogyne flaccida, a horticulturally and medicinally important endangered orchid of Eastern Himalaya
title_full	Protocorm-like bodies and plant regeneration from foliar explants of Coelogyne flaccida, a horticulturally and medicinally important endangered orchid of Eastern Himalaya
title_fullStr	Protocorm-like bodies and plant regeneration from foliar explants of Coelogyne flaccida, a horticulturally and medicinally important endangered orchid of Eastern Himalaya
title_full_unstemmed	Protocorm-like bodies and plant regeneration from foliar explants of Coelogyne flaccida, a horticulturally and medicinally important endangered orchid of Eastern Himalaya
title_sort	protocorm-like bodies and plant regeneration from foliar explants of coelogyne flaccida, a horticulturally and medicinally important endangered orchid of eastern himalaya
title_alt	Protocorm-like bodies and plant regeneration from foliar explants of Coelogyne flaccida, a horticulturally and medicinally important endangered orchid of Eastern Himalaya
publisher	Universidad de Costa Rica
publishDate	2015
url	https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/20747
work_keys_str_mv	AT dekaylank protocormlikebodiesandplantregenerationfromfoliarexplantsofcoelogyneflaccidaahorticulturallyandmedicinallyimportantendangeredorchidofeasternhimalaya AT silsujit protocormlikebodiesandplantregenerationfromfoliarexplantsofcoelogyneflaccidaahorticulturallyandmedicinallyimportantendangeredorchidofeasternhimalaya
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Protocorm-like bodies and plant regeneration from foliar explants of Coelogyne flaccida, a horticulturally and medicinally important endangered orchid of Eastern Himalaya

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