Adquisición de competencia para la micropagación de Stanhopea tigrina, Laelia anceps, Epidendrum veroscriptum y Cattleya x Esbetts (Orchidaceae)
Regeneration protocols were established from the in vitro culture of protocormos of Stanhopea tigrina, Epidendrum veroscriptum, Laelia anceps and Cattleya x esbetts using the Murashige and Skoog culture medium added with different concentrations of N6-benzyladenine (BA) (0, 1, 3, y 5 mg/l) y 2,4 dic...
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Universidad de Costa Rica
2015
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Acceso en línea: | https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/19612 |
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LANKESTERIANA196122021-06-09T20:53:23Z Adquisición de competencia para la micropagación de Stanhopea tigrina, Laelia anceps, Epidendrum veroscriptum y Cattleya x Esbetts (Orchidaceae) Adquisición de competencia para la micropagación de Stanhopea tigrina, Laelia anceps, Epidendrum veroscriptum y Cattleya x Esbetts (Orchidaceae) Tinoco Juárez, Mario Sinaí Mata Rosas, Martín orquídeas protocormo adquisición de competencia cultivo de tejidos micropropagación orquídeas protocormo adquisición de competencia cultivo de tejidos micropropagación Regeneration protocols were established from the in vitro culture of protocormos of Stanhopea tigrina, Epidendrum veroscriptum, Laelia anceps and Cattleya x esbetts using the Murashige and Skoog culture medium added with different concentrations of N6-benzyladenine (BA) (0, 1, 3, y 5 mg/l) y 2,4 dichlorofenoxiacetic acid (2,4-D). The regeneration and formation of new plantlets was achieved by multiple shoot and also through protocorm like bodies (PLBs). For each species the best concentration of growth regulator, as well as the time of acquisition of competence for the highest shoot formation and/or PLBs were deter- mined. As far as we know there are no reports with reference to the acquisition of competence within the Orchidaceae family that allow the production of many individuals within the shortest time and reducing the financial cost. This could form the base that covers their commercial demand and also contribute to the con- servation and sustainable use of mainly wild species. Regeneration protocols were established from the in vitro culture of protocormos of Stanhopea tigrina, Epidendrum veroscriptum, Laelia anceps and Cattleya x esbetts using the Murashige and Skoog culture medium added with different concentrations of N6-benzyladenine (BA) (0, 1, 3, y 5 mg/l) y 2,4 dichlorofenoxiacetic acid (2,4-D). The regeneration and formation of new plantlets was achieved by multiple shoot and also through protocorm like bodies (PLBs). For each species the best concentration of growth regulator, as well as the time of acquisition of competence for the highest shoot formation and/or PLBs were deter- mined. As far as we know there are no reports with reference to the acquisition of competence within the Orchidaceae family that allow the production of many individuals within the shortest time and reducing the financial cost. This could form the base that covers their commercial demand and also contribute to the con- servation and sustainable use of mainly wild species. Universidad de Costa Rica 2015-06-17 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article application/pdf https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/19612 10.15517/lank.v7i1-2.19612 Lankesteriana: International Journal on Orchidology; 2007: Lankesteriana: Volumen 7, Número 1-2 Lankesteriana: International Journal on Orchidology; 2007: Lankesteriana: Volumen 7, Número 1-2 2215-2067 1409-3871 spa https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/19612/19699 |
institution |
Universidad de Costa Rica |
collection |
Lankesteriana: International Journal on Orchidology |
language |
spa |
format |
Online |
author |
Tinoco Juárez, Mario Sinaí Mata Rosas, Martín |
spellingShingle |
Tinoco Juárez, Mario Sinaí Mata Rosas, Martín Adquisición de competencia para la micropagación de Stanhopea tigrina, Laelia anceps, Epidendrum veroscriptum y Cattleya x Esbetts (Orchidaceae) |
author_facet |
Tinoco Juárez, Mario Sinaí Mata Rosas, Martín |
author_sort |
Tinoco Juárez, Mario Sinaí |
description |
Regeneration protocols were established from the in vitro culture of protocormos of Stanhopea tigrina, Epidendrum veroscriptum, Laelia anceps and Cattleya x esbetts using the Murashige and Skoog culture medium added with different concentrations of N6-benzyladenine (BA) (0, 1, 3, y 5 mg/l) y 2,4 dichlorofenoxiacetic acid (2,4-D). The regeneration and formation of new plantlets was achieved by multiple shoot and also through protocorm like bodies (PLBs). For each species the best concentration of growth regulator, as well as the time of acquisition of competence for the highest shoot formation and/or PLBs were deter- mined. As far as we know there are no reports with reference to the acquisition of competence within the Orchidaceae family that allow the production of many individuals within the shortest time and reducing the financial cost. This could form the base that covers their commercial demand and also contribute to the con- servation and sustainable use of mainly wild species. |
title |
Adquisición de competencia para la micropagación de Stanhopea tigrina, Laelia anceps, Epidendrum veroscriptum y Cattleya x Esbetts (Orchidaceae) |
title_short |
Adquisición de competencia para la micropagación de Stanhopea tigrina, Laelia anceps, Epidendrum veroscriptum y Cattleya x Esbetts (Orchidaceae) |
title_full |
Adquisición de competencia para la micropagación de Stanhopea tigrina, Laelia anceps, Epidendrum veroscriptum y Cattleya x Esbetts (Orchidaceae) |
title_fullStr |
Adquisición de competencia para la micropagación de Stanhopea tigrina, Laelia anceps, Epidendrum veroscriptum y Cattleya x Esbetts (Orchidaceae) |
title_full_unstemmed |
Adquisición de competencia para la micropagación de Stanhopea tigrina, Laelia anceps, Epidendrum veroscriptum y Cattleya x Esbetts (Orchidaceae) |
title_sort |
adquisición de competencia para la micropagación de stanhopea tigrina, laelia anceps, epidendrum veroscriptum y cattleya x esbetts (orchidaceae) |
title_alt |
Adquisición de competencia para la micropagación de Stanhopea tigrina, Laelia anceps, Epidendrum veroscriptum y Cattleya x Esbetts (Orchidaceae) |
publisher |
Universidad de Costa Rica |
publishDate |
2015 |
url |
https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/19612 |
work_keys_str_mv |
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_version_ |
1810112939184619520 |