Adquisición de competencia para la micropagación de Stanhopea tigrina, Laelia anceps, Epidendrum veroscriptum y Cattleya x Esbetts (Orchidaceae)

Regeneration protocols were established from the in vitro culture of protocormos of Stanhopea tigrina, Epidendrum veroscriptum, Laelia anceps and Cattleya x esbetts using the Murashige and Skoog culture medium added with different concentrations of N6-benzyladenine (BA) (0, 1, 3, y 5 mg/l) y 2,4 dic...

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Autores principales: Tinoco Juárez, Mario Sinaí, Mata Rosas, Martín
Formato: Online
Idioma:spa
Publicado: Universidad de Costa Rica 2015
Acceso en línea:https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/19612
id LANKESTERIANA19612
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spelling LANKESTERIANA196122021-06-09T20:53:23Z Adquisición de competencia para la micropagación de Stanhopea tigrina, Laelia anceps, Epidendrum veroscriptum y Cattleya x Esbetts (Orchidaceae) Adquisición de competencia para la micropagación de Stanhopea tigrina, Laelia anceps, Epidendrum veroscriptum y Cattleya x Esbetts (Orchidaceae) Tinoco Juárez, Mario Sinaí Mata Rosas, Martín orquídeas protocormo adquisición de competencia cultivo de tejidos micropropagación orquídeas protocormo adquisición de competencia cultivo de tejidos micropropagación  Regeneration protocols were established from the in vitro culture of protocormos of Stanhopea tigrina, Epidendrum veroscriptum, Laelia anceps and Cattleya x esbetts using the Murashige and Skoog culture medium added with different concentrations of N6-benzyladenine (BA) (0, 1, 3, y 5 mg/l) y 2,4 dichlorofenoxiacetic acid (2,4-D). The regeneration and formation of new plantlets was achieved by multiple shoot and also through protocorm like bodies (PLBs). For each species the best concentration of growth regulator, as well as the time of acquisition of competence for the highest shoot formation and/or PLBs were deter- mined. As far as we know there are no reports with reference to the acquisition of competence within the Orchidaceae family that allow the production of many individuals within the shortest time and reducing the financial cost. This could form the base that covers their commercial demand and also contribute to the con- servation and sustainable use of mainly wild species.  Regeneration protocols were established from the in vitro culture of protocormos of Stanhopea tigrina, Epidendrum veroscriptum, Laelia anceps and Cattleya x esbetts using the Murashige and Skoog culture medium added with different concentrations of N6-benzyladenine (BA) (0, 1, 3, y 5 mg/l) y 2,4 dichlorofenoxiacetic acid (2,4-D). The regeneration and formation of new plantlets was achieved by multiple shoot and also through protocorm like bodies (PLBs). For each species the best concentration of growth regulator, as well as the time of acquisition of competence for the highest shoot formation and/or PLBs were deter- mined. As far as we know there are no reports with reference to the acquisition of competence within the Orchidaceae family that allow the production of many individuals within the shortest time and reducing the financial cost. This could form the base that covers their commercial demand and also contribute to the con- servation and sustainable use of mainly wild species.  Universidad de Costa Rica 2015-06-17 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article application/pdf https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/19612 10.15517/lank.v7i1-2.19612 Lankesteriana: International Journal on Orchidology; 2007: Lankesteriana: Volumen 7, Número 1-2 Lankesteriana: International Journal on Orchidology; 2007: Lankesteriana: Volumen 7, Número 1-2 2215-2067 1409-3871 spa https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/19612/19699
institution Universidad de Costa Rica
collection Lankesteriana: International Journal on Orchidology
language spa
format Online
author Tinoco Juárez, Mario Sinaí
Mata Rosas, Martín
spellingShingle Tinoco Juárez, Mario Sinaí
Mata Rosas, Martín
Adquisición de competencia para la micropagación de Stanhopea tigrina, Laelia anceps, Epidendrum veroscriptum y Cattleya x Esbetts (Orchidaceae)
author_facet Tinoco Juárez, Mario Sinaí
Mata Rosas, Martín
author_sort Tinoco Juárez, Mario Sinaí
description Regeneration protocols were established from the in vitro culture of protocormos of Stanhopea tigrina, Epidendrum veroscriptum, Laelia anceps and Cattleya x esbetts using the Murashige and Skoog culture medium added with different concentrations of N6-benzyladenine (BA) (0, 1, 3, y 5 mg/l) y 2,4 dichlorofenoxiacetic acid (2,4-D). The regeneration and formation of new plantlets was achieved by multiple shoot and also through protocorm like bodies (PLBs). For each species the best concentration of growth regulator, as well as the time of acquisition of competence for the highest shoot formation and/or PLBs were deter- mined. As far as we know there are no reports with reference to the acquisition of competence within the Orchidaceae family that allow the production of many individuals within the shortest time and reducing the financial cost. This could form the base that covers their commercial demand and also contribute to the con- servation and sustainable use of mainly wild species. 
title Adquisición de competencia para la micropagación de Stanhopea tigrina, Laelia anceps, Epidendrum veroscriptum y Cattleya x Esbetts (Orchidaceae)
title_short Adquisición de competencia para la micropagación de Stanhopea tigrina, Laelia anceps, Epidendrum veroscriptum y Cattleya x Esbetts (Orchidaceae)
title_full Adquisición de competencia para la micropagación de Stanhopea tigrina, Laelia anceps, Epidendrum veroscriptum y Cattleya x Esbetts (Orchidaceae)
title_fullStr Adquisición de competencia para la micropagación de Stanhopea tigrina, Laelia anceps, Epidendrum veroscriptum y Cattleya x Esbetts (Orchidaceae)
title_full_unstemmed Adquisición de competencia para la micropagación de Stanhopea tigrina, Laelia anceps, Epidendrum veroscriptum y Cattleya x Esbetts (Orchidaceae)
title_sort adquisición de competencia para la micropagación de stanhopea tigrina, laelia anceps, epidendrum veroscriptum y cattleya x esbetts (orchidaceae)
title_alt Adquisición de competencia para la micropagación de Stanhopea tigrina, Laelia anceps, Epidendrum veroscriptum y Cattleya x Esbetts (Orchidaceae)
publisher Universidad de Costa Rica
publishDate 2015
url https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/19612
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