Propagación in vitro y aclimatización de Euchile mariae (Ames) Withner (Orchidaceae)

Orchid micropropagation of species Euchile mariae was achieved from the in vitro culture of protocorms sections, obtained from the germination of seeds. The top and bottom protocorms sections, used as explants, were cultivated in modified MS culture medium, added with different concentrations of α-n...

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Autores principales:	Suárez-Quijada, Iris, Hernández-Altamirano, Mabel, Chávez-Ávila, Víctor, Sandoval-Zapotilla, Estela, Martínez-Palacios, Alejandro
Formato:	Online
Idioma:	spa
Publicado:	Universidad de Costa Rica 2015
Acceso en línea:	https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/19609

id	LANKESTERIANA19609
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spelling	LANKESTERIANA196092021-06-09T20:53:42Z Propagación in vitro y aclimatización de Euchile mariae (Ames) Withner (Orchidaceae) Propagación in vitro y aclimatización de Euchile mariae (Ames) Withner (Orchidaceae) Suárez-Quijada, Iris Hernández-Altamirano, Mabel Chávez-Ávila, Víctor Sandoval-Zapotilla, Estela Martínez-Palacios, Alejandro micropropagación orquídeas Euchile PLB’s aclimatización conservación ex situ micropropagación orquídeas Euchile PLB’s aclimatización conservación ex situ Orchid micropropagation of species Euchile mariae was achieved from the in vitro culture of protocorms sections, obtained from the germination of seeds. The top and bottom protocorms sections, used as explants, were cultivated in modified MS culture medium, added with different concentrations of α-naph- thaleneacetic acid (NAA) and 6-benzylaminopurine (BA). The morphogenetic response obtained of both types of explants, was the formation of protocorm like bodies (PLB’s), that gave place to the formation of new plantlets. Of two tested explants the biggest formation of PLB’s was obtained from the bottom proto- corms sections. The acclimatization of the plantlets was successful, with a 100% percent survival. Plant cell tissue culture represents a useful alternative for the study, conservation and massive propagation of this and other endangered species. Orchid micropropagation of species Euchile mariae was achieved from the in vitro culture of protocorms sections, obtained from the germination of seeds. The top and bottom protocorms sections, used as explants, were cultivated in modified MS culture medium, added with different concentrations of α-naph- thaleneacetic acid (NAA) and 6-benzylaminopurine (BA). The morphogenetic response obtained of both types of explants, was the formation of protocorm like bodies (PLB’s), that gave place to the formation of new plantlets. Of two tested explants the biggest formation of PLB’s was obtained from the bottom proto- corms sections. The acclimatization of the plantlets was successful, with a 100% percent survival. Plant cell tissue culture represents a useful alternative for the study, conservation and massive propagation of this and other endangered species. Universidad de Costa Rica 2015-06-17 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article application/pdf https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/19609 10.15517/lank.v7i1-2.19609 Lankesteriana: International Journal on Orchidology; 2007: Lankesteriana: Volumen 7, Número 1-2 Lankesteriana: International Journal on Orchidology; 2007: Lankesteriana: Volumen 7, Número 1-2 2215-2067 1409-3871 spa https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/19609/19693
institution	Universidad de Costa Rica
collection	Lankesteriana: International Journal on Orchidology
language	spa
format	Online
author	Suárez-Quijada, Iris Hernández-Altamirano, Mabel Chávez-Ávila, Víctor Sandoval-Zapotilla, Estela Martínez-Palacios, Alejandro
spellingShingle	Suárez-Quijada, Iris Hernández-Altamirano, Mabel Chávez-Ávila, Víctor Sandoval-Zapotilla, Estela Martínez-Palacios, Alejandro Propagación in vitro y aclimatización de Euchile mariae (Ames) Withner (Orchidaceae)
author_facet	Suárez-Quijada, Iris Hernández-Altamirano, Mabel Chávez-Ávila, Víctor Sandoval-Zapotilla, Estela Martínez-Palacios, Alejandro
author_sort	Suárez-Quijada, Iris
description	Orchid micropropagation of species Euchile mariae was achieved from the in vitro culture of protocorms sections, obtained from the germination of seeds. The top and bottom protocorms sections, used as explants, were cultivated in modified MS culture medium, added with different concentrations of α-naph- thaleneacetic acid (NAA) and 6-benzylaminopurine (BA). The morphogenetic response obtained of both types of explants, was the formation of protocorm like bodies (PLB’s), that gave place to the formation of new plantlets. Of two tested explants the biggest formation of PLB’s was obtained from the bottom proto- corms sections. The acclimatization of the plantlets was successful, with a 100% percent survival. Plant cell tissue culture represents a useful alternative for the study, conservation and massive propagation of this and other endangered species.
title	Propagación in vitro y aclimatización de Euchile mariae (Ames) Withner (Orchidaceae)
title_short	Propagación in vitro y aclimatización de Euchile mariae (Ames) Withner (Orchidaceae)
title_full	Propagación in vitro y aclimatización de Euchile mariae (Ames) Withner (Orchidaceae)
title_fullStr	Propagación in vitro y aclimatización de Euchile mariae (Ames) Withner (Orchidaceae)
title_full_unstemmed	Propagación in vitro y aclimatización de Euchile mariae (Ames) Withner (Orchidaceae)
title_sort	propagación in vitro y aclimatización de euchile mariae (ames) withner (orchidaceae)
title_alt	Propagación in vitro y aclimatización de Euchile mariae (Ames) Withner (Orchidaceae)
publisher	Universidad de Costa Rica
publishDate	2015
url	https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/19609
work_keys_str_mv	AT suarezquijadairis propagacioninvitroyaclimatizaciondeeuchilemariaeameswithnerorchidaceae AT hernandezaltamiranomabel propagacioninvitroyaclimatizaciondeeuchilemariaeameswithnerorchidaceae AT chavezavilavictor propagacioninvitroyaclimatizaciondeeuchilemariaeameswithnerorchidaceae AT sandovalzapotillaestela propagacioninvitroyaclimatizaciondeeuchilemariaeameswithnerorchidaceae AT martinezpalaciosalejandro propagacioninvitroyaclimatizaciondeeuchilemariaeameswithnerorchidaceae
_version_	1810112938759946240

Propagación in vitro y aclimatización de Euchile mariae (Ames) Withner (Orchidaceae)

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