Desarrollo de cápsulas y germinación in vitro de Phragmipedium humboldtii, P. longifolium y P. pearcei
Slipper orchids belonging to the genus Phragmipedium (subfam. Cypripedioideae) are seriously threatened and therefore listed in Appendix I of CITES. Less research has been conducted in this genus than in others belonging to the same subfamily. In this work, we evaluated development of capsules (seed...
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| Formato: | Online |
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Universidad de Costa Rica
2015
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| Acceso en línea: | https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/19529 |
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LANKESTERIANA19529 |
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ojs |
| institution |
Universidad de Costa Rica |
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Lankesteriana: International Journal on Orchidology |
| language |
spa |
| format |
Online |
| author |
Muñoz, Melania Jiménez, Víctor |
| spellingShingle |
Muñoz, Melania Jiménez, Víctor Desarrollo de cápsulas y germinación in vitro de Phragmipedium humboldtii, P. longifolium y P. pearcei |
| author_facet |
Muñoz, Melania Jiménez, Víctor |
| author_sort |
Muñoz, Melania |
| description |
Slipper orchids belonging to the genus Phragmipedium (subfam. Cypripedioideae) are seriously threatened and therefore listed in Appendix I of CITES. Less research has been conducted in this genus than in others belonging to the same subfamily. In this work, we evaluated development of capsules (seed pods) from Phragmipedium humboldtii, P. longifolium and P. peaarcei from the time of pollination until opening. Moreover, seed viability was tested with the tetrazolium method in each of the capsules that were subsequently used to evaluate the effect of light and two culture media (Knudson C vs. Murashige and Skoog half concentrated) on in vitro asymbiotic germination and seedling growth. 100% of the pollinated flowers developed capsules, which differentiated in terms of length and diameter among species. While the length of the capsules remained constant during development, their diameter increased during the first 6-8 weeks and then stopped. Time required for maturity and opening of the capsules also varied among species (31 weeks in P. humboldtii, 16 weeks in P. longifolium and 9.5 weeks in P. percei). Seed viability differed among species as well, averaging 34.3% in P. humboldtii, 44.7% in P. longifolium and 82.3% in P. pearcei. Furthermore, seed viability of each capsule was used to adjust the germination rate measured in each case. While very few P. humboldtii viable seeds germinated under the conditions tested (2.9%), better results were observed in the other two species (close to 40% germination). No significant effect of light/darkness regime or of culture medium was observed on germination. However, better growth of the germinated embryos was observed with the Knudson C medium and darkness conditions. Further subculture of the gro- wing plantlets under light conditions induced development of roots and allowed successful acclimatization of seedlings in the greenhouse. |
| title |
Desarrollo de cápsulas y germinación in vitro de Phragmipedium humboldtii, P. longifolium y P. pearcei |
| title_short |
Desarrollo de cápsulas y germinación in vitro de Phragmipedium humboldtii, P. longifolium y P. pearcei |
| title_full |
Desarrollo de cápsulas y germinación in vitro de Phragmipedium humboldtii, P. longifolium y P. pearcei |
| title_fullStr |
Desarrollo de cápsulas y germinación in vitro de Phragmipedium humboldtii, P. longifolium y P. pearcei |
| title_full_unstemmed |
Desarrollo de cápsulas y germinación in vitro de Phragmipedium humboldtii, P. longifolium y P. pearcei |
| title_sort |
desarrollo de cápsulas y germinación in vitro de phragmipedium humboldtii, p. longifolium y p. pearcei |
| title_alt |
Desarrollo de cápsulas y germinación in vitro de Phragmipedium humboldtii, P. longifolium y P. pearcei |
| publisher |
Universidad de Costa Rica |
| publishDate |
2015 |
| url |
https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/19529 |
| work_keys_str_mv |
AT munozmelania desarrollodecapsulasygerminacioninvitrodephragmipediumhumboldtiiplongifoliumyppearcei AT jimenezvictor desarrollodecapsulasygerminacioninvitrodephragmipediumhumboldtiiplongifoliumyppearcei |
| _version_ |
1810112936329347072 |
| spelling |
LANKESTERIANA195292021-06-09T20:55:32Z Desarrollo de cápsulas y germinación in vitro de Phragmipedium humboldtii, P. longifolium y P. pearcei Desarrollo de cápsulas y germinación in vitro de Phragmipedium humboldtii, P. longifolium y P. pearcei Muñoz, Melania Jiménez, Víctor Phragmipedium orquídeas terrestres polinización desarrollo de cápsulas cloruro de tetrazolio germinación in vitro. Phragmipedium orquídeas terrestres polinización desarrollo de cápsulas cloruro de tetrazolio germinación in vitro. Slipper orchids belonging to the genus Phragmipedium (subfam. Cypripedioideae) are seriously threatened and therefore listed in Appendix I of CITES. Less research has been conducted in this genus than in others belonging to the same subfamily. In this work, we evaluated development of capsules (seed pods) from Phragmipedium humboldtii, P. longifolium and P. peaarcei from the time of pollination until opening. Moreover, seed viability was tested with the tetrazolium method in each of the capsules that were subsequently used to evaluate the effect of light and two culture media (Knudson C vs. Murashige and Skoog half concentrated) on in vitro asymbiotic germination and seedling growth. 100% of the pollinated flowers developed capsules, which differentiated in terms of length and diameter among species. While the length of the capsules remained constant during development, their diameter increased during the first 6-8 weeks and then stopped. Time required for maturity and opening of the capsules also varied among species (31 weeks in P. humboldtii, 16 weeks in P. longifolium and 9.5 weeks in P. percei). Seed viability differed among species as well, averaging 34.3% in P. humboldtii, 44.7% in P. longifolium and 82.3% in P. pearcei. Furthermore, seed viability of each capsule was used to adjust the germination rate measured in each case. While very few P. humboldtii viable seeds germinated under the conditions tested (2.9%), better results were observed in the other two species (close to 40% germination). No significant effect of light/darkness regime or of culture medium was observed on germination. However, better growth of the germinated embryos was observed with the Knudson C medium and darkness conditions. Further subculture of the gro- wing plantlets under light conditions induced development of roots and allowed successful acclimatization of seedlings in the greenhouse. Slipper orchids belonging to the genus Phragmipedium (subfam. Cypripedioideae) are seriously threatened and therefore listed in Appendix I of CITES. Less research has been conducted in this genus than in others belonging to the same subfamily. In this work, we evaluated development of capsules (seed pods) from Phragmipedium humboldtii, P. longifolium and P. peaarcei from the time of pollination until opening. Moreover, seed viability was tested with the tetrazolium method in each of the capsules that were subsequently used to evaluate the effect of light and two culture media (Knudson C vs. Murashige and Skoog half concentrated) on in vitro asymbiotic germination and seedling growth. 100% of the pollinated flowers developed capsules, which differentiated in terms of length and diameter among species. While the length of the capsules remained constant during development, their diameter increased during the first 6-8 weeks and then stopped. Time required for maturity and opening of the capsules also varied among species (31 weeks in P. humboldtii, 16 weeks in P. longifolium and 9.5 weeks in P. percei). Seed viability differed among species as well, averaging 34.3% in P. humboldtii, 44.7% in P. longifolium and 82.3% in P. pearcei. Furthermore, seed viability of each capsule was used to adjust the germination rate measured in each case. While very few P. humboldtii viable seeds germinated under the conditions tested (2.9%), better results were observed in the other two species (close to 40% germination). No significant effect of light/darkness regime or of culture medium was observed on germination. However, better growth of the germinated embryos was observed with the Knudson C medium and darkness conditions. Further subculture of the gro- wing plantlets under light conditions induced development of roots and allowed successful acclimatization of seedlings in the greenhouse. Universidad de Costa Rica 2015-06-17 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article application/pdf https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/19529 10.15517/lank.v7i1-2.19529 Lankesteriana: International Journal on Orchidology; 2007: Lankesteriana: Volumen 7, Número 1-2 Lankesteriana: International Journal on Orchidology; 2007: Lankesteriana: Volumen 7, Número 1-2 2215-2067 1409-3871 spa https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/19529/19609 |