Propagation of Dendrobium aggregatum by green capsule culture

An efficient protocol for propagation of Dendrobium aggregatum using the axenic immature seeds, derived from green capsule, was developed. The immature embryos from 120 days old capsules after pollination were germinated on Murashige and Skoog (MS) medium supplement with various concentration of BAP...

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Autores principales:	Vijayakumar, S., Rajalkshmi, G., Kalimuthu, K.
Formato:	Online
Idioma:	eng
Publicado:	Universidad de Costa Rica 2013
Acceso en línea:	https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/11763

id	LANKESTERIANA11763
record_format	ojs
spelling	LANKESTERIANA117632022-07-20T01:01:07Z Propagation of Dendrobium aggregatum by green capsule culture Propagation of Dendrobium aggregatum by green capsule culture Vijayakumar, S. Rajalkshmi, G. Kalimuthu, K. dendrobium protocorm like bodies acclimatization in vitro dendrobium protocorm like bodies acclimatization in vitro An efficient protocol for propagation of Dendrobium aggregatum using the axenic immature seeds, derived from green capsule, was developed. The immature embryos from 120 days old capsules after pollination were germinated on Murashige and Skoog (MS) medium supplement with various concentration of BAP alone or in combination with NAA along with coconut water, and the same media were used for induction, multiplication, elongation and rooting in vitro shoots. MS medium with the addition of 3% sucrose 1.5 mg L-1 Benzyl amino purine (BAP) and 15% coconut water (CW) favoured the higher rate of germination, more number of protocorm like bodies, production of maximum number of shoots, elongation of shoots, as well as root formation. During acclimatization, 95% of the plantlets survived after one month. An efficient protocol for propagation of Dendrobium aggregatum using the axenic immature seeds, derived from green capsule, was developed. The immature embryos from 120 days old capsules after pollination were germinated on Murashige and Skoog (MS) medium supplement with various concentration of BAP alone or in combination with NAA along with coconut water, and the same media were used for induction, multiplication, elongation and rooting in vitro shoots. MS medium with the addition of 3% sucrose 1.5 mg L-1 Benzyl amino purine (BAP) and 15% coconut water (CW) favoured the higher rate of germination, more number of protocorm like bodies, production of maximum number of shoots, elongation of shoots, as well as root formation. During acclimatization, 95% of the plantlets survived after one month. Universidad de Costa Rica 2013-09-04 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article application/pdf https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/11763 10.15517/lank.v0i0.11763 Lankesteriana: International Journal on Orchidology; 2012: Lankesteriana: Volumen 12, Número 2 Lankesteriana: International Journal on Orchidology; 2012: Lankesteriana: Volumen 12, Número 2 2215-2067 1409-3871 eng https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/11763/11080
institution	Universidad de Costa Rica
collection	Lankesteriana: International Journal on Orchidology
language	eng
format	Online
author	Vijayakumar, S. Rajalkshmi, G. Kalimuthu, K.
spellingShingle	Vijayakumar, S. Rajalkshmi, G. Kalimuthu, K. Propagation of Dendrobium aggregatum by green capsule culture
author_facet	Vijayakumar, S. Rajalkshmi, G. Kalimuthu, K.
author_sort	Vijayakumar, S.
description	An efficient protocol for propagation of Dendrobium aggregatum using the axenic immature seeds, derived from green capsule, was developed. The immature embryos from 120 days old capsules after pollination were germinated on Murashige and Skoog (MS) medium supplement with various concentration of BAP alone or in combination with NAA along with coconut water, and the same media were used for induction, multiplication, elongation and rooting in vitro shoots. MS medium with the addition of 3% sucrose 1.5 mg L-1 Benzyl amino purine (BAP) and 15% coconut water (CW) favoured the higher rate of germination, more number of protocorm like bodies, production of maximum number of shoots, elongation of shoots, as well as root formation. During acclimatization, 95% of the plantlets survived after one month.
title	Propagation of Dendrobium aggregatum by green capsule culture
title_short	Propagation of Dendrobium aggregatum by green capsule culture
title_full	Propagation of Dendrobium aggregatum by green capsule culture
title_fullStr	Propagation of Dendrobium aggregatum by green capsule culture
title_full_unstemmed	Propagation of Dendrobium aggregatum by green capsule culture
title_sort	propagation of dendrobium aggregatum by green capsule culture
title_alt	Propagation of Dendrobium aggregatum by green capsule culture
publisher	Universidad de Costa Rica
publishDate	2013
url	https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/11763
work_keys_str_mv	AT vijayakumars propagationofdendrobiumaggregatumbygreencapsuleculture AT rajalkshmig propagationofdendrobiumaggregatumbygreencapsuleculture AT kalimuthuk propagationofdendrobiumaggregatumbygreencapsuleculture
_version_	1810112907523915776

Propagation of Dendrobium aggregatum by green capsule culture

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