Possibilities of breeding teak (Tectona grandisL.f.) in Costa Rica assisted by AFLP markers

Teak tree (Tectona grandis L.f.) is a tropical tree specie naturally distributed in Southeast Asia, where it is also widely planted. It is planted as exotic specie in Africa, South and Central America. Tree improvement activities have been initiated in several countries including Costa Rica, which i...

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Autores principales: Araya-Valverde, Emanuel, Murillo-Gamboa, Olman, Aguilar, Gabriel, Rocha, Oscar, Woolbright, Scott, Keim, Paul
Formato: Online
Idioma:spa
Publicado: Editorial Tecnológica de Costa Rica 2012
Acceso en línea:https://revistas.tec.ac.cr/index.php/kuru/article/view/542
id KURU542
record_format ojs
spelling KURU5422015-12-16T03:19:34Z Possibilities of breeding teak (Tectona grandisL.f.) in Costa Rica assisted by AFLP markers Araya-Valverde, Emanuel Murillo-Gamboa, Olman Aguilar, Gabriel Rocha, Oscar Woolbright, Scott Keim, Paul AFLP Breeding Gene marker Progeny test Genetic distance Tectona grandis Costa Rica Teak tree (Tectona grandis L.f.) is a tropical tree specie naturally distributed in Southeast Asia, where it is also widely planted. It is planted as exotic specie in Africa, South and Central America. Tree improvement activities have been initiated in several countries including Costa Rica, which it has become the largest teak developer in Latin America. Teak sprouts were collected in a 4-yearold progeny test from the best two and the worst two families for volume as well as for stem quality traits. A DNA extraction protocol described before was modified in order to yield high quality DNA.AFLP reactions were performed as described previously. Seven selective primer combinations (EACG + M-CCG, E-ACT +M-CCG, E-AGC + M-CCG, E-ACG + M-CTC, E-ACT + M-CTC, E-AGC + M-CTC, E-AGG + M-CCA) resulted in 330 scoreable markers. A large number of markers were obtained when families where analyzed separately from its performance. Genetic distances based on AFLP markers frequencies were used to construct a dendrogram among families. The best quality performer families grouped widely separated from the worst performers, sharing 61.63% of their genetic elements. In volume, worst performer families grouped sharing 45.85% of their genetic elements. Editorial Tecnológica de Costa Rica 2012-11-28 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.tec.ac.cr/index.php/kuru/article/view/542 Revista Forestal Mesoamericana Kurú; Vol. 2 Núm. 5 (2005); pág. 12-18 2215-2504 spa https://revistas.tec.ac.cr/index.php/kuru/article/view/542/468
institution Tecnológico de Costa Rica
collection Revista Forestal Mesoamaericana
language spa
format Online
author Araya-Valverde, Emanuel
Murillo-Gamboa, Olman
Aguilar, Gabriel
Rocha, Oscar
Woolbright, Scott
Keim, Paul
spellingShingle Araya-Valverde, Emanuel
Murillo-Gamboa, Olman
Aguilar, Gabriel
Rocha, Oscar
Woolbright, Scott
Keim, Paul
Possibilities of breeding teak (Tectona grandisL.f.) in Costa Rica assisted by AFLP markers
author_facet Araya-Valverde, Emanuel
Murillo-Gamboa, Olman
Aguilar, Gabriel
Rocha, Oscar
Woolbright, Scott
Keim, Paul
author_sort Araya-Valverde, Emanuel
description Teak tree (Tectona grandis L.f.) is a tropical tree specie naturally distributed in Southeast Asia, where it is also widely planted. It is planted as exotic specie in Africa, South and Central America. Tree improvement activities have been initiated in several countries including Costa Rica, which it has become the largest teak developer in Latin America. Teak sprouts were collected in a 4-yearold progeny test from the best two and the worst two families for volume as well as for stem quality traits. A DNA extraction protocol described before was modified in order to yield high quality DNA.AFLP reactions were performed as described previously. Seven selective primer combinations (EACG + M-CCG, E-ACT +M-CCG, E-AGC + M-CCG, E-ACG + M-CTC, E-ACT + M-CTC, E-AGC + M-CTC, E-AGG + M-CCA) resulted in 330 scoreable markers. A large number of markers were obtained when families where analyzed separately from its performance. Genetic distances based on AFLP markers frequencies were used to construct a dendrogram among families. The best quality performer families grouped widely separated from the worst performers, sharing 61.63% of their genetic elements. In volume, worst performer families grouped sharing 45.85% of their genetic elements.
title Possibilities of breeding teak (Tectona grandisL.f.) in Costa Rica assisted by AFLP markers
title_short Possibilities of breeding teak (Tectona grandisL.f.) in Costa Rica assisted by AFLP markers
title_full Possibilities of breeding teak (Tectona grandisL.f.) in Costa Rica assisted by AFLP markers
title_fullStr Possibilities of breeding teak (Tectona grandisL.f.) in Costa Rica assisted by AFLP markers
title_full_unstemmed Possibilities of breeding teak (Tectona grandisL.f.) in Costa Rica assisted by AFLP markers
title_sort possibilities of breeding teak (tectona grandisl.f.) in costa rica assisted by aflp markers
publisher Editorial Tecnológica de Costa Rica
publishDate 2012
url https://revistas.tec.ac.cr/index.php/kuru/article/view/542
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