Effect of growth conditions on Ophiostoma piceae (Münch) H. & P. Syd. and Ophiostoma floccosum Math-Käärik albino strains culture morphology
Blue stain in Pinus radiata D. Don wood is predominantly caused by Ophiostoma genus fungi. Stained wood causes significant economic losses to forestry and timber industries. Currently, different chemical fungicides are used to prevent the occurrence of blue stain fungi. However, recent reports have...
| Autores principales: | , , |
|---|---|
| Formato: | Online |
| Idioma: | eng |
| Publicado: |
Editorial Tecnológica de Costa Rica
2014
|
| Acceso en línea: | https://revistas.tec.ac.cr/index.php/kuru/article/view/2095 |
| id |
KURU2095 |
|---|---|
| record_format |
ojs |
| spelling |
KURU20952020-05-07T22:35:07Z Effect of growth conditions on Ophiostoma piceae (Münch) H. & P. Syd. and Ophiostoma floccosum Math-Käärik albino strains culture morphology Effect of growth conditions on Ophiostoma piceae (Münch) H. & P. Syd. and Ophiostoma floccosum Math-Käärik albino strains culture morphology Berrocal-Jiménez, Alexander Navarrete, José Oviedo, Claudia Ophiostoma piceae Ophiostoma floccosum wood-staining fungi fungal dimorphism inoculum size Chile. Ophiostoma piceae Ophiostoma floccosum wood-staining fungi fungal dimorphism inoculum size Chile. Blue stain in Pinus radiata D. Don wood is predominantly caused by Ophiostoma genus fungi. Stained wood causes significant economic losses to forestry and timber industries. Currently, different chemical fungicides are used to prevent the occurrence of blue stain fungi. However, recent reports have questioned the environmental friendliness of these fungicides. For this reason, the use of biological control, in particular with albino strains of the Ophiostoma genus, appears to be an environmentally safe and a technically feasible alternative to work with. The potential field application of this technology would use a formulation containing the albino fungus-the bioactive ingredient- in its yeast like form. However, some Ophiostoma strains cultures present a marked filamentous morphology in liquid culture medium, affecting the efficiency in the production of the bioactive ingredient. The hypothesis of this work presumed the possibility to control the morphology of Ophiostoma cultures, through the modification of growth conditions in liquid medium. The effect of inoculum size, growth temperature and agitation rate on the morphology of PcF2A29 Ophiostoma piceae and FlF1A55 Ophiostoma floccosum albino strains in liquid culture medium, was studied. A 23 factorial design was employed. The results showed that the initial inoculum size had a statistically significant effect on yeast-like growth morphology in both strains, whereas the temperature only produced a significant effect in PcF2A29 O. piceae strain. Blue stain in Pinus radiata D. Don wood is predominantly caused by Ophiostoma genus fungi. Stained wood causes significant economic losses to forestry and timber industries. Currently, different chemical fungicides are used to prevent the occurrence of blue stain fungi. However, recent reports have questioned the environmental friendliness of these fungicides. For this reason, the use of biological control, in particular with albino strains of the Ophiostoma genus, appears to be an environmentally safe and a technically feasible alternative to work with. The potential field application of this technology would use a formulation containing the albino fungus-the bioactive ingredient- in its yeast like form. However, some Ophiostoma strains cultures present a marked filamentous morphology in liquid culture medium, affecting the efficiency in the production of the bioactive ingredient. The hypothesis of this work presumed the possibility to control the morphology of Ophiostoma cultures, through the modification of growth conditions in liquid medium. The effect of inoculum size, growth temperature and agitation rate on the morphology of PcF2A29 Ophiostoma piceae and FlF1A55 Ophiostoma floccosum albino strains in liquid culture medium, was studied. A 23 factorial design was employed. The results showed that the initial inoculum size had a statistically significant effect on yeast-like growth morphology in both strains, whereas the temperature only produced a significant effect in PcF2A29 O. piceae strain. Editorial Tecnológica de Costa Rica 2014-12-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.tec.ac.cr/index.php/kuru/article/view/2095 10.18845/rfmk.v12i28.2095 Revista Forestal Mesoamericana Kurú; Vol. 12 Núm. 28 (2015): Enero- Junio 2015; 4-12 2215-2504 eng https://revistas.tec.ac.cr/index.php/kuru/article/view/2095/1931 Derechos de autor 2014 Revista Forestal Mesoamericana Kurú |
| institution |
Tecnológico de Costa Rica |
| collection |
Revista Forestal Mesoamaericana |
| language |
eng |
| format |
Online |
| author |
Berrocal-Jiménez, Alexander Navarrete, José Oviedo, Claudia |
| spellingShingle |
Berrocal-Jiménez, Alexander Navarrete, José Oviedo, Claudia Effect of growth conditions on Ophiostoma piceae (Münch) H. & P. Syd. and Ophiostoma floccosum Math-Käärik albino strains culture morphology |
| author_facet |
Berrocal-Jiménez, Alexander Navarrete, José Oviedo, Claudia |
| author_sort |
Berrocal-Jiménez, Alexander |
| description |
Blue stain in Pinus radiata D. Don wood is predominantly caused by Ophiostoma genus fungi. Stained wood causes significant economic losses to forestry and timber industries. Currently, different chemical fungicides are used to prevent the occurrence of blue stain fungi. However, recent reports have questioned the environmental friendliness of these fungicides. For this reason, the use of biological control, in particular with albino strains of the Ophiostoma genus, appears to be an environmentally safe and a technically feasible alternative to work with. The potential field application of this technology would use a formulation containing the albino fungus-the bioactive ingredient- in its yeast like form. However, some Ophiostoma strains cultures present a marked filamentous morphology in liquid culture medium, affecting the efficiency in the production of the bioactive ingredient. The hypothesis of this work presumed the possibility to control the morphology of Ophiostoma cultures, through the modification of growth conditions in liquid medium. The effect of inoculum size, growth temperature and agitation rate on the morphology of PcF2A29 Ophiostoma piceae and FlF1A55 Ophiostoma floccosum albino strains in liquid culture medium, was studied. A 23 factorial design was employed. The results showed that the initial inoculum size had a statistically significant effect on yeast-like growth morphology in both strains, whereas the temperature only produced a significant effect in PcF2A29 O. piceae strain. |
| title |
Effect of growth conditions on Ophiostoma piceae (Münch) H. & P. Syd. and Ophiostoma floccosum Math-Käärik albino strains culture morphology |
| title_short |
Effect of growth conditions on Ophiostoma piceae (Münch) H. & P. Syd. and Ophiostoma floccosum Math-Käärik albino strains culture morphology |
| title_full |
Effect of growth conditions on Ophiostoma piceae (Münch) H. & P. Syd. and Ophiostoma floccosum Math-Käärik albino strains culture morphology |
| title_fullStr |
Effect of growth conditions on Ophiostoma piceae (Münch) H. & P. Syd. and Ophiostoma floccosum Math-Käärik albino strains culture morphology |
| title_full_unstemmed |
Effect of growth conditions on Ophiostoma piceae (Münch) H. & P. Syd. and Ophiostoma floccosum Math-Käärik albino strains culture morphology |
| title_sort |
effect of growth conditions on ophiostoma piceae (münch) h. & p. syd. and ophiostoma floccosum math-käärik albino strains culture morphology |
| title_alt |
Effect of growth conditions on Ophiostoma piceae (Münch) H. & P. Syd. and Ophiostoma floccosum Math-Käärik albino strains culture morphology |
| publisher |
Editorial Tecnológica de Costa Rica |
| publishDate |
2014 |
| url |
https://revistas.tec.ac.cr/index.php/kuru/article/view/2095 |
| work_keys_str_mv |
AT berrocaljimenezalexander effectofgrowthconditionsonophiostomapiceaemunchhpsydandophiostomafloccosummathkaarikalbinostrainsculturemorphology AT navarretejose effectofgrowthconditionsonophiostomapiceaemunchhpsydandophiostomafloccosummathkaarikalbinostrainsculturemorphology AT oviedoclaudia effectofgrowthconditionsonophiostomapiceaemunchhpsydandophiostomafloccosummathkaarikalbinostrainsculturemorphology |
| _version_ |
1805400215533912064 |