Evaluation of the development of infective structures and the effect of Beauveria bassiana on the growth of tomato seedlings (Solanum lycopersicum) under high-temperature conditions
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Universidad Nacional, Costa Rica
2025
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| Online Access: | https://www.revistas.una.ac.cr/index.php/uniciencia/article/view/18936 |
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UNICIENCIA18936 |
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Universidad Nacional de Costa Rica |
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Uniciencia |
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spa |
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Online |
| author |
Villalobos-Álvarez, Estefani Mariel Castro-Vásquez, Ruth Vega-Corrales, Luis Pérez-Molina, Junior Pastor |
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Villalobos-Álvarez, Estefani Mariel Castro-Vásquez, Ruth Vega-Corrales, Luis Pérez-Molina, Junior Pastor Evaluation of the development of infective structures and the effect of Beauveria bassiana on the growth of tomato seedlings (Solanum lycopersicum) under high-temperature conditions |
| author_facet |
Villalobos-Álvarez, Estefani Mariel Castro-Vásquez, Ruth Vega-Corrales, Luis Pérez-Molina, Junior Pastor |
| author_sort |
Villalobos-Álvarez, Estefani Mariel |
| title |
Evaluation of the development of infective structures and the effect of Beauveria bassiana on the growth of tomato seedlings (Solanum lycopersicum) under high-temperature conditions |
| title_short |
Evaluation of the development of infective structures and the effect of Beauveria bassiana on the growth of tomato seedlings (Solanum lycopersicum) under high-temperature conditions |
| title_full |
Evaluation of the development of infective structures and the effect of Beauveria bassiana on the growth of tomato seedlings (Solanum lycopersicum) under high-temperature conditions |
| title_fullStr |
Evaluation of the development of infective structures and the effect of Beauveria bassiana on the growth of tomato seedlings (Solanum lycopersicum) under high-temperature conditions |
| title_full_unstemmed |
Evaluation of the development of infective structures and the effect of Beauveria bassiana on the growth of tomato seedlings (Solanum lycopersicum) under high-temperature conditions |
| title_sort |
evaluation of the development of infective structures and the effect of beauveria bassiana on the growth of tomato seedlings (solanum lycopersicum) under high-temperature conditions |
| title_alt |
Evaluación del desarrollo de estructuras infectivas y efecto de Beauveria bassiana en el crecimiento de plántulas de tomate (Solanum lycopersicum) bajo condiciones de alta temperatura Avaliação do desenvolvimento de estruturas infectantes e efeito de Beauveria bassiana no crescimento de mudas de tomateiro (Solanum lycopersicum) sob condições de altas temperaturas |
| publisher |
Universidad Nacional, Costa Rica |
| publishDate |
2025 |
| url |
https://www.revistas.una.ac.cr/index.php/uniciencia/article/view/18936 |
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AT villalobosalvarezestefanimariel evaluationofthedevelopmentofinfectivestructuresandtheeffectofbeauveriabassianaonthegrowthoftomatoseedlingssolanumlycopersicumunderhightemperatureconditions AT castrovasquezruth evaluationofthedevelopmentofinfectivestructuresandtheeffectofbeauveriabassianaonthegrowthoftomatoseedlingssolanumlycopersicumunderhightemperatureconditions AT vegacorralesluis evaluationofthedevelopmentofinfectivestructuresandtheeffectofbeauveriabassianaonthegrowthoftomatoseedlingssolanumlycopersicumunderhightemperatureconditions AT perezmolinajuniorpastor evaluationofthedevelopmentofinfectivestructuresandtheeffectofbeauveriabassianaonthegrowthoftomatoseedlingssolanumlycopersicumunderhightemperatureconditions AT villalobosalvarezestefanimariel evaluaciondeldesarrollodeestructurasinfectivasyefectodebeauveriabassianaenelcrecimientodeplantulasdetomatesolanumlycopersicumbajocondicionesdealtatemperatura AT castrovasquezruth evaluaciondeldesarrollodeestructurasinfectivasyefectodebeauveriabassianaenelcrecimientodeplantulasdetomatesolanumlycopersicumbajocondicionesdealtatemperatura AT vegacorralesluis evaluaciondeldesarrollodeestructurasinfectivasyefectodebeauveriabassianaenelcrecimientodeplantulasdetomatesolanumlycopersicumbajocondicionesdealtatemperatura AT perezmolinajuniorpastor evaluaciondeldesarrollodeestructurasinfectivasyefectodebeauveriabassianaenelcrecimientodeplantulasdetomatesolanumlycopersicumbajocondicionesdealtatemperatura AT villalobosalvarezestefanimariel avaliacaododesenvolvimentodeestruturasinfectanteseefeitodebeauveriabassiananocrescimentodemudasdetomateirosolanumlycopersicumsobcondicoesdealtastemperaturas AT castrovasquezruth avaliacaododesenvolvimentodeestruturasinfectanteseefeitodebeauveriabassiananocrescimentodemudasdetomateirosolanumlycopersicumsobcondicoesdealtastemperaturas AT vegacorralesluis avaliacaododesenvolvimentodeestruturasinfectanteseefeitodebeauveriabassiananocrescimentodemudasdetomateirosolanumlycopersicumsobcondicoesdealtastemperaturas AT perezmolinajuniorpastor avaliacaododesenvolvimentodeestruturasinfectanteseefeitodebeauveriabassiananocrescimentodemudasdetomateirosolanumlycopersicumsobcondicoesdealtastemperaturas |
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UNICIENCIA189362025-03-12T00:07:48Z Evaluation of the development of infective structures and the effect of Beauveria bassiana on the growth of tomato seedlings (Solanum lycopersicum) under high-temperature conditions Evaluación del desarrollo de estructuras infectivas y efecto de Beauveria bassiana en el crecimiento de plántulas de tomate (Solanum lycopersicum) bajo condiciones de alta temperatura Avaliação do desenvolvimento de estruturas infectantes e efeito de Beauveria bassiana no crescimento de mudas de tomateiro (Solanum lycopersicum) sob condições de altas temperaturas Villalobos-Álvarez, Estefani Mariel Castro-Vásquez, Ruth Vega-Corrales, Luis Pérez-Molina, Junior Pastor Thermo-tolerance entomopathogenic fungi biologic control growth promoter physiological stress termo-tolerancia hongos entomopatógenos control biológico promotor de crecimiento estrés fisiológico termotolerância fungos entomopatogênicos controle biológico promotor de crescimento estresse fisiológico [Objective] The paper aimed to evaluate in vitro development of infective structures of Beauveria spp. strains and their effect on the growth of tomato seedlings (Solanum lycopersicum) under high-temperature conditions. [Methodology] The development of five strains was compared under both optimal and high-temperature conditions (up to 35°C) regarding the production of conidia, mycelium growth, and germination. Subsequently, the effect of the strain with the highest yield on the growth of seedlings of S. lycopersicum, cultivar 'Gladiator', was assessed under the same temperature conditions. Furthermore, each strain was identified using PCR amplification of the nuclear intergenic region Bloc and elongation factor EF1-α. [Results] Strains were identified as Beauveria bassiana of neotropical lineage. No significant differences were found in the conidia production between treatments (p-value > 0.05), as opposed to mycelial growth. Minor but significant changes were observed in root-to-surface ratios but not in any other evaluated variables, and only for seedlings treated with strain H-31. [Conclusions] The entomopathogenic potential of B. bassiana was determined under environmental stress conditions, such as high temperatures typical of tropical climates. This study also lays the groundwork for the bioprospecting of thermotolerant microorganisms with agrobiotechnological potential in global warming scenarios. [Objetivo] Se evaluó el desarrollo in vitro de estructuras infectivas de cepas de Beauveria spp. y su efecto en el crecimiento de plántulas de tomate (Solanum lycopersicum) en condiciones de alta temperatura. [Metodología] Se comparó el desarrollo de 5 cepas de Beauveria spp. en condiciones óptimas y de alta temperatura (hasta 35 °C) en relación con la producción de conidios, crecimiento del micelio y germinación. Posteriormente, se evaluó el efecto de la cepa con mejores resultados de desarrollo en el crecimiento de plántulas de S. lycopersicum, cultivar 'Gladiator', bajo las mismas condiciones de temperatura. Cada cepa fue identificada por PCR con la amplificación de la zona intergénica nuclear Bloc y el factor de elongación EF1-α. [Resultados] Las cepas se identificaron como Beauveria bassiana de linaje neotropical. No se encontraron diferencias significativas en la producción de conidios entre tratamientos (p-value >0.05) a diferencia del crecimiento del micelio. Se observaron cambios leves, pero significativos en las relaciones de raíz a superficie, no así en las demás variables evaluadas y solo para las plántulas a las que se les aplicó la cepa H-31 [Conclusiones] Se determinó el potencial entomopatógeno de B. bassiana en condiciones de estrés ambiental como las altas temperaturas del clima tropical. Se establece, además, la base para la bioprospección de microorganismos termotolerantes con potencial agrobiotecnológico en escenarios de calentamiento global. [Objetivo] Foi avaliado o desenvolvimento in vitro de estruturas infecciosas de cepas de Beauveria spp. e seu efeito no crescimento de mudas de tomate (Solanum lycopersicum) sob condições de altas temperaturas. [Metodologia] O desenvolvimento de 5 cepas de Beauveria spp. em condições ideais e alta temperatura (até 35 °C) em relação à produção de conídios, crescimento micelial e germinação. Posteriormente, avaliou-se o efeito da cepa com melhores resultados de desenvolvimento no crescimento de mudas de S. lycopersicum, cultivar 'Gladiator', nas mesmas condições de temperatura. Cada cepa foi identificada por PCR com a amplificação da região intergênica nuclear Bloc e do fator de alongamento EF1-α. [Resultados] As cepas foram identificadas como Beauveria bassiana de linhagem neotropical. Não foram encontradas diferenças significativas na produção de conídios entre os tratamentos (valor de p >0,05) diferentemente do crescimento do micélio. Foram observadas pequenas, porém significativas, alterações nas relações raiz-superfície, mas não nas demais variáveis avaliadas e apenas para as mudas nas quais foi aplicada a cepa H-31 [Conclusões] Foi determinado o potencial entomopatogênico de B. bassiana em condições de estresse ambiental, como as altas temperaturas do clima tropical. Estabelecem-se também as bases para a bioprospecção de microrganismos termotolerantes com potencial agrobiotecnológico em cenários de aquecimento global. Universidad Nacional, Costa Rica 2025-01-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Papers evaluated by academic peers Artículos evaluados por pares académicos application/pdf text/html application/epub+zip https://www.revistas.una.ac.cr/index.php/uniciencia/article/view/18936 10.15359/ru.39-1.2 Uniciencia; Vol. 39 No. 1 (2025): Uniciencia. January-December, 2025; 1-15 Uniciencia; Vol. 39 Núm. 1 (2025): Uniciencia. January-December, 2025; 1-15 Uniciencia; v. 39 n. 1 (2025): Uniciencia. January-December, 2025; 1-15 2215-3470 spa https://www.revistas.una.ac.cr/index.php/uniciencia/article/view/18936/32970 https://www.revistas.una.ac.cr/index.php/uniciencia/article/view/18936/32971 https://www.revistas.una.ac.cr/index.php/uniciencia/article/view/18936/32972 Copyright (c) 2025 Shared by Journal and Authors (CC-BY-NC-ND) https://creativecommons.org/licenses/by-nc-nd/4.0 |