Antimicrobial activity of Cymbopogon citratus (Poaceae) essential oil on Streptococcus mutans biofilm and cytotoxic effect on keratinocytes and fibroblasts
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| Format: | Online |
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Universidad de Costa Rica
2018
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| Online Access: | https://revistas.ucr.ac.cr/index.php/rbt/article/view/33140 |
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RBT33140 |
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RBT331402022-06-09T18:17:36Z Antimicrobial activity of Cymbopogon citratus (Poaceae) essential oil on Streptococcus mutans biofilm and cytotoxic effect on keratinocytes and fibroblasts Ortega Cuadros, Mailen Tofiño Rivera, Adriana Patricia Merini, Luciano Jose Martinez Pabon, Maria Cecilia biofilms terpenes dental caries phytotherapy Cymbopogon citratus essentail oils. Dental caries is a pathology of multifactorial origin and currently natural products are an efficient alternative treatment; The work sought to evaluate the antimicrobial activity of the Cymbopogon citratus essential oil and the citral and myrcene components against Streptococcus mutans ATCC UA159, as well as their cytotoxicity on keratinocytes and human fibroblasts. The viability effect against Streptococcus mutans on biofilms was evaluated through exposure to the three substances by using the MBEC technique-high-throughput at concentrations of 1, 0.1, and 0.01 µg/mL and chlorhexidine as positive control. The cytotoxicity of the compounds was evaluated on keratinocytes and fibroblasts through the MTT reduction technique, using 0.5 mM H2O2 as cell-death control (negative control) and ethanol 1% as vehicle control (positive control). The three substances evaluated had effects on the viability of Streptococcus mutans with mortality between 74% and 96%, without significant difference among them (p > 0.393); additionally, no cytotoxicity was evident on keratinocytes and fibroblasts in a 24-h treatment. The substances evaluated showed significant antimicrobial effects; hence, these should be studied further as potential co-adjuvants to prevent dental caries that cause minor adverse effects Universidad de Costa Rica 2018-12-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article application/pdf text/html application/vnd.openxmlformats-officedocument.wordprocessingml.document image/tiff image/tiff image/tiff image/tiff application/vnd.openxmlformats-officedocument.spreadsheetml.sheet https://revistas.ucr.ac.cr/index.php/rbt/article/view/33140 10.15517/rbt.v66i4.33140 Revista de Biología Tropical; Vol. 66 No. 4 (2018): Volume 66 – Regular number 4 – December 2018; 1519–1529 Revista de Biología Tropical; Vol. 66 Núm. 4 (2018): Volumen 66 – Número regular 4 – Diciembre 2018; 1519–1529 Revista Biología Tropical; Vol. 66 N.º 4 (2018): Volume 66 – Regular number 4 – December 2018; 1519–1529 2215-2075 0034-7744 10.15517/rbt.v66i4 eng https://revistas.ucr.ac.cr/index.php/rbt/article/view/33140/34514 https://revistas.ucr.ac.cr/index.php/rbt/article/view/33140/34515 https://revistas.ucr.ac.cr/index.php/rbt/article/view/33140/35820 https://revistas.ucr.ac.cr/index.php/rbt/article/view/33140/35821 https://revistas.ucr.ac.cr/index.php/rbt/article/view/33140/35822 https://revistas.ucr.ac.cr/index.php/rbt/article/view/33140/35823 https://revistas.ucr.ac.cr/index.php/rbt/article/view/33140/35824 https://revistas.ucr.ac.cr/index.php/rbt/article/view/33140/35825 Copyright (c) 2018 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0 |
| institution |
Universidad de Costa Rica |
| collection |
Revista de Biología Tropical |
| language |
eng |
| format |
Online |
| author |
Ortega Cuadros, Mailen Tofiño Rivera, Adriana Patricia Merini, Luciano Jose Martinez Pabon, Maria Cecilia |
| spellingShingle |
Ortega Cuadros, Mailen Tofiño Rivera, Adriana Patricia Merini, Luciano Jose Martinez Pabon, Maria Cecilia Antimicrobial activity of Cymbopogon citratus (Poaceae) essential oil on Streptococcus mutans biofilm and cytotoxic effect on keratinocytes and fibroblasts |
| author_facet |
Ortega Cuadros, Mailen Tofiño Rivera, Adriana Patricia Merini, Luciano Jose Martinez Pabon, Maria Cecilia |
| author_sort |
Ortega Cuadros, Mailen |
| title |
Antimicrobial activity of Cymbopogon citratus (Poaceae) essential oil on Streptococcus mutans biofilm and cytotoxic effect on keratinocytes and fibroblasts |
| title_short |
Antimicrobial activity of Cymbopogon citratus (Poaceae) essential oil on Streptococcus mutans biofilm and cytotoxic effect on keratinocytes and fibroblasts |
| title_full |
Antimicrobial activity of Cymbopogon citratus (Poaceae) essential oil on Streptococcus mutans biofilm and cytotoxic effect on keratinocytes and fibroblasts |
| title_fullStr |
Antimicrobial activity of Cymbopogon citratus (Poaceae) essential oil on Streptococcus mutans biofilm and cytotoxic effect on keratinocytes and fibroblasts |
| title_full_unstemmed |
Antimicrobial activity of Cymbopogon citratus (Poaceae) essential oil on Streptococcus mutans biofilm and cytotoxic effect on keratinocytes and fibroblasts |
| title_sort |
antimicrobial activity of cymbopogon citratus (poaceae) essential oil on streptococcus mutans biofilm and cytotoxic effect on keratinocytes and fibroblasts |
| publisher |
Universidad de Costa Rica |
| publishDate |
2018 |
| url |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/33140 |
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