A technique for extraction and Thin Layer Chromatography visualization of fecal bile acids applied to neotropical felid scats

A technique for extraction and Thin Layer Chromatography visualization of fecal bile acids applied to neotropical felid scats

Bibliographic Details
Main Authors: Cazón Narvaez, Ada Virginia, Sühring, Silvia Susana
Format: Online
Language:eng
Published: Universidad de Costa Rica 1999
Online Access:https://revistas.ucr.ac.cr/index.php/rbt/article/view/33042
id RBT33042
record_format ojs
spelling RBT330422022-05-30T19:16:23Z A technique for extraction and Thin Layer Chromatography visualization of fecal bile acids applied to neotropical felid scats A technique for extraction and Thin Layer Chromatography visualization of fecal bile acids applied to neotropical felid scats Cazón Narvaez, Ada Virginia Sühring, Silvia Susana Thin layer chromatography TLC bile acids felids scats Fecal bile acid patterns have been used successfully to identify scats. Neotropical felid scats are capable of this biochemical identification because they present low concentrations of plant pigments that would interfere in fecal bile acids detection. However, neotropical felid scats have poor quantities of bile acids, so we developed in this work a proper technique for their extraction, visualization and determination. Twenty eighth feces of seven different felid species, collected from Zoological and Wildlife Parks, were dried and pulverized. The procedure for analyzing feces is: Take one g of pulverized feces and shake for 3 hr at room temperature in 20 ml benzene : methanol; filter and evaporate to 5 ml. Spot on TLC plate and develop in toluene :acetic acid- :water. Dry and visualize with anisaldehyde. Field collected scats could be identified by the bile acids pattern revealed by this specific technique and ,then, used as a source of information for distribution, density and food habits studies. Universidad de Costa Rica 1999-03-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article application/pdf https://revistas.ucr.ac.cr/index.php/rbt/article/view/33042 10.15517/rbt.v47i1-2.33042 Revista de Biología Tropical; Vol. 47 No. 1-2 (1999): Volume 47 – Regular number 1-2 – March 1999; 245–249 Revista de Biología Tropical; Vol. 47 Núm. 1-2 (1999): Volumen 47 – Número regular 1-2 – Marzo 1999; 245–249 Revista Biología Tropical; Vol. 47 N.º 1-2 (1999): Volume 47 – Regular number 1-2 – March 1999; 245–249 2215-2075 0034-7744 10.15517/rbt.v47i1-2 eng https://revistas.ucr.ac.cr/index.php/rbt/article/view/33042/32494 Copyright (c) 1999 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0
institution Universidad de Costa Rica
collection Revista de Biología Tropical
language eng
format Online
author Cazón Narvaez, Ada Virginia
Sühring, Silvia Susana
spellingShingle Cazón Narvaez, Ada Virginia
Sühring, Silvia Susana
A technique for extraction and Thin Layer Chromatography visualization of fecal bile acids applied to neotropical felid scats
author_facet Cazón Narvaez, Ada Virginia
Sühring, Silvia Susana
author_sort Cazón Narvaez, Ada Virginia
title A technique for extraction and Thin Layer Chromatography visualization of fecal bile acids applied to neotropical felid scats
title_short A technique for extraction and Thin Layer Chromatography visualization of fecal bile acids applied to neotropical felid scats
title_full A technique for extraction and Thin Layer Chromatography visualization of fecal bile acids applied to neotropical felid scats
title_fullStr A technique for extraction and Thin Layer Chromatography visualization of fecal bile acids applied to neotropical felid scats
title_full_unstemmed A technique for extraction and Thin Layer Chromatography visualization of fecal bile acids applied to neotropical felid scats
title_sort technique for extraction and thin layer chromatography visualization of fecal bile acids applied to neotropical felid scats
title_alt A technique for extraction and Thin Layer Chromatography visualization of fecal bile acids applied to neotropical felid scats
publisher Universidad de Costa Rica
publishDate 1999
url https://revistas.ucr.ac.cr/index.php/rbt/article/view/33042
work_keys_str_mv AT cazonnarvaezadavirginia atechniqueforextractionandthinlayerchromatographyvisualizationoffecalbileacidsappliedtoneotropicalfelidscats
AT suhringsilviasusana atechniqueforextractionandthinlayerchromatographyvisualizationoffecalbileacidsappliedtoneotropicalfelidscats
AT cazonnarvaezadavirginia techniqueforextractionandthinlayerchromatographyvisualizationoffecalbileacidsappliedtoneotropicalfelidscats
AT suhringsilviasusana techniqueforextractionandthinlayerchromatographyvisualizationoffecalbileacidsappliedtoneotropicalfelidscats
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