Purification and Some Properties of Hemagglutinating Protein Mutina from Bushmaster Lachesis muta Snake Venom
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| Format: | Online |
| Language: | eng |
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Universidad de Costa Rica
1986
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| Online Access: | https://revistas.ucr.ac.cr/index.php/rbt/article/view/24355 |
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RBT243552022-05-19T18:23:52Z Purification and Some Properties of Hemagglutinating Protein Mutina from Bushmaster Lachesis muta Snake Venom Purification and Some Properties of Hemagglutinating Protein Mutina from Bushmaster Lachesis muta Snake Venom Gómez Leiva, M. A. Aragón-Ortíz, F. Lachesis muta snake venom induced aggregation of bromelain sensitized human erythrocytes al a concentration of 1 mg/ml. The hemagglutinating protein was purified by DEAE-Sephadex A-50 column chromatography. Polyacrylamide gel electrophoresis revealed at least three bands, whereas SDS electrophoresis in the presence of 2-mercaptoethanol showed a single one. Isoelectric focusing revealed hemagglutinating activity in the range of pH 3-8. The maximun peak (mutina) al pH 5.5. This fraction was active in agglutinating human RBC of types A, B. O Rh (+) and B. O Rh (-).One mM EDTA and 1 mM Ca++ did not alter the agglutinating time significantly. Lactase and inositol inhibited the agglutination of A, S, O Rh (+) and B, O Rh (-) human RBC. The present study showed the non specificity of the hemagglutinating activity of mutina. It was also shown that mutina is a non-mitogenic protein. Universidad de Costa Rica 1986-06-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article application/pdf https://revistas.ucr.ac.cr/index.php/rbt/article/view/24355 Revista de Biología Tropical; Vol. 34 No. 1 (1986): Volume 34 – Regular number 1 – June 1986; 49–53 Revista de Biología Tropical; Vol. 34 Núm. 1 (1986): Volumen 34 – Número regular 1 – Junio 1986; 49–53 Revista Biología Tropical; Vol. 34 N.º 1 (1986): Volume 34 – Regular number 1 – June 1986; 49–53 2215-2075 0034-7744 10.15517/rbt.v34i1 eng https://revistas.ucr.ac.cr/index.php/rbt/article/view/24355/24454 Copyright (c) 1986 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0 |
| institution |
Universidad de Costa Rica |
| collection |
Revista de Biología Tropical |
| language |
eng |
| format |
Online |
| author |
Gómez Leiva, M. A. Aragón-Ortíz, F. |
| spellingShingle |
Gómez Leiva, M. A. Aragón-Ortíz, F. Purification and Some Properties of Hemagglutinating Protein Mutina from Bushmaster Lachesis muta Snake Venom |
| author_facet |
Gómez Leiva, M. A. Aragón-Ortíz, F. |
| author_sort |
Gómez Leiva, M. A. |
| title |
Purification and Some Properties of Hemagglutinating Protein Mutina from Bushmaster Lachesis muta Snake Venom |
| title_short |
Purification and Some Properties of Hemagglutinating Protein Mutina from Bushmaster Lachesis muta Snake Venom |
| title_full |
Purification and Some Properties of Hemagglutinating Protein Mutina from Bushmaster Lachesis muta Snake Venom |
| title_fullStr |
Purification and Some Properties of Hemagglutinating Protein Mutina from Bushmaster Lachesis muta Snake Venom |
| title_full_unstemmed |
Purification and Some Properties of Hemagglutinating Protein Mutina from Bushmaster Lachesis muta Snake Venom |
| title_sort |
purification and some properties of hemagglutinating protein mutina from bushmaster lachesis muta snake venom |
| title_alt |
Purification and Some Properties of Hemagglutinating Protein Mutina from Bushmaster Lachesis muta Snake Venom |
| publisher |
Universidad de Costa Rica |
| publishDate |
1986 |
| url |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/24355 |
| work_keys_str_mv |
AT gomezleivama purificationandsomepropertiesofhemagglutinatingproteinmutinafrombushmasterlachesismutasnakevenom AT aragonortizf purificationandsomepropertiesofhemagglutinatingproteinmutinafrombushmasterlachesismutasnakevenom |
| _version_ |
1810114955027939328 |